ELFN1_MOUSE
ID ELFN1_MOUSE Reviewed; 828 AA.
AC Q8C8T7;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Protein ELFN1;
DE AltName: Full=Extracellular leucine-rich repeat and fibronectin type-III domain-containing protein 1;
DE AltName: Full=Protein phosphatase 1 regulatory subunit 28;
DE Flags: Precursor;
GN Name=Elfn1; Synonyms=Ppp1r28;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Retina;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=23042292; DOI=10.1126/science.1222482;
RA Sylwestrak E.L., Ghosh A.;
RT "Elfn1 regulates target-specific release probability at CA1-interneuron
RT synapses.";
RL Science 338:536-540(2012).
CC -!- FUNCTION: Postsynaptic protein that regulates circuit dynamics in the
CC central nervous system by modulating the temporal dynamics of
CC interneuron recruitment. Specifically present in excitatory synapses
CC onto oriens-lacunosum molecular (OLM) interneurons and acts as a
CC regulator of presynaptic release probability to direct the formation of
CC highly facilitating pyramidal-OLM synapses. Inhibits phosphatase
CC activity of protein phosphatase 1 (PP1) complexes.
CC {ECO:0000269|PubMed:23042292}.
CC -!- SUBUNIT: Interacts with PPP1CA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}. Cell projection, dendrite
CC {ECO:0000269|PubMed:23042292}. Note=Localizes to excitatory synapses
CC onto somatostatin (Sst)-containing oriens-lacunosum moleculare (O-LM)
CC interneurons.
CC -!- TISSUE SPECIFICITY: Selectively expressed in perialvear somatostatin
CC (Sst)-containing interneurons. {ECO:0000269|PubMed:23042292}.
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DR EMBL; AK044511; BAC31957.1; -; mRNA.
DR EMBL; BC059029; AAH59029.1; -; mRNA.
DR CCDS; CCDS19816.1; -.
DR RefSeq; NP_780731.1; NM_175522.3.
DR RefSeq; XP_006504764.1; XM_006504701.3.
DR RefSeq; XP_006504765.1; XM_006504702.3.
DR RefSeq; XP_006504766.1; XM_006504703.3.
DR RefSeq; XP_006504767.1; XM_006504704.3.
DR RefSeq; XP_017176371.1; XM_017320882.1.
DR AlphaFoldDB; Q8C8T7; -.
DR SMR; Q8C8T7; -.
DR BioGRID; 232502; 1.
DR IntAct; Q8C8T7; 1.
DR STRING; 10090.ENSMUSP00000053869; -.
DR GlyConnect; 2624; 2 N-Linked glycans (1 site).
DR GlyGen; Q8C8T7; 6 sites, 2 N-linked glycans (1 site).
DR iPTMnet; Q8C8T7; -.
DR PhosphoSitePlus; Q8C8T7; -.
DR MaxQB; Q8C8T7; -.
DR PaxDb; Q8C8T7; -.
DR PRIDE; Q8C8T7; -.
DR ProteomicsDB; 277818; -.
DR Antibodypedia; 67952; 59 antibodies from 16 providers.
DR DNASU; 243312; -.
DR Ensembl; ENSMUST00000050519; ENSMUSP00000053869; ENSMUSG00000048988.
DR GeneID; 243312; -.
DR KEGG; mmu:243312; -.
DR UCSC; uc009ahi.2; mouse.
DR CTD; 392617; -.
DR MGI; MGI:2442479; Elfn1.
DR VEuPathDB; HostDB:ENSMUSG00000048988; -.
DR eggNOG; ENOG502QVFI; Eukaryota.
DR GeneTree; ENSGT00940000161391; -.
DR HOGENOM; CLU_018770_0_0_1; -.
DR InParanoid; Q8C8T7; -.
DR OMA; CDSPPGV; -.
DR OrthoDB; 190870at2759; -.
DR PhylomeDB; Q8C8T7; -.
DR TreeFam; TF332887; -.
DR BioGRID-ORCS; 243312; 2 hits in 77 CRISPR screens.
DR ChiTaRS; Elfn1; mouse.
DR PRO; PR:Q8C8T7; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8C8T7; protein.
DR Bgee; ENSMUSG00000048988; Expressed in retinal neural layer and 79 other tissues.
DR Genevisible; Q8C8T7; MM.
DR GO; GO:0030425; C:dendrite; IDA:UniProtKB.
DR GO; GO:0060076; C:excitatory synapse; IDA:UniProtKB.
DR GO; GO:0031012; C:extracellular matrix; IBA:GO_Central.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004864; F:protein phosphatase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0050808; P:synapse organization; IMP:UniProtKB.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR000483; Cys-rich_flank_reg_C.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF13855; LRR_8; 1.
DR SMART; SM00369; LRR_TYP; 4.
DR SMART; SM00082; LRRCT; 1.
DR PROSITE; PS51450; LRR; 5.
PE 2: Evidence at transcript level;
KW Cell projection; Glycoprotein; Leucine-rich repeat; Membrane;
KW Phosphoprotein; Protein phosphatase inhibitor; Reference proteome; Repeat;
KW Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..828
FT /note="Protein ELFN1"
FT /id="PRO_0000343739"
FT TOPO_DOM 26..418
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 419..439
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 440..828
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 61..82
FT /note="LRR 1"
FT REPEAT 85..106
FT /note="LRR 2"
FT REPEAT 109..130
FT /note="LRR 3"
FT REPEAT 133..154
FT /note="LRR 4"
FT REPEAT 157..178
FT /note="LRR 5"
FT DOMAIN 190..253
FT /note="LRRCT"
FT DOMAIN 312..399
FT /note="Fibronectin type-III"
FT REGION 258..293
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 627..674
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 697..731
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 266..287
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 636..653
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 712..727
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 460
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P0C7U0"
FT MOD_RES 646
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P0C7U0"
FT CARBOHYD 85
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 90
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 122
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 210
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 376
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 828 AA; 90815 MW; D07E6734C156E03D CRC64;
MAGHGWGTAW VLVAAATLLH AGGLAQGDCW LIEGDKGFVW LAICSQNQPP YEAIPQQINN
TIVDLRLNEN RIRSVQYASL SRFGNLTYLN LTKNEIGYIE DGAFSGQFNL QVLQLGYNRL
RNLTEGMLRG LSKLEYLYLQ ANLIEVVMAS AFWECPNIVN IDLSMNRIQQ LGSGTFAGLT
KLSVCEIYSN PFYCSCELLG FLRWLAAFTN ATQTHDRVQC ESPPVYAGYF LLGQGRHGHQ
RSILSKLQSV CTEGSYTAEV LGPPRPVPGR SQPGHSPPPP PPEPSDMPCA DDECFSGDGT
TPLVILTTLV PQTEARPSMK VKQLTQNSAT IMVQLPSPFN RMYTLEQYNN SKSFTVSKLT
QPQEEIRLTN LYTLTNYTYC VVSTSSGTHH NHTCLTICLP KPPSPPGPVP SPSTATHYIM
TILGCLFGMV LVLGAVYYCL RKRRRQEEKH KKAVAAAAGS LKKTIIELKY GPEIEAPGLA
PLTQGPLLGP EAVTRIPYLP AATSDVEQYK LVESSETPKA TKGNYIEVRT GEPQERRGCE
LSRPGEPQSS VAEISTIAKE VDRVNQIINN CIDALKSEST SFQGAKSGAV SAAEPQLVLL
SEPLASKHSF LSPVYKDAFG HGGLQRHHSV EAAPGPPRAS TSSSGSARSP RTFRAEATGT
HKAPATETKY IEKSSPVPET ILTVTPAATV LRAEADKSRQ YGEHRHSYPG SHPAEPPAPP
PPPPTHEGLG GRKASILEPL TRPRPRDLVY SQLSPQYHNL SYSSSPEYTC RASPSIWERL
RLSRRRHKDD AEFMAAGHAL RKKVQFAKDE DLHDILDYWK GVSAQHKS
