ELG1_DROME
ID ELG1_DROME Reviewed; 1162 AA.
AC Q86BP6; Q8MSZ2; Q9VUZ2;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Enhanced level of genomic instability 1 {ECO:0000305};
GN Name=elg1 {ECO:0000312|FlyBase:FBgn0036574};
GN ORFNames=CG16838 {ECO:0000312|FlyBase:FBgn0036574};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAM49842.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM49842.1};
RC TISSUE=Ovary {ECO:0000312|EMBL:AAM49842.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN THE ELG1 RFC-LIKE COMPLEX, INTERACTION WITH THE
RP ENOK COMPLEX, INTERACTION WITH BR140, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=27198229; DOI=10.1101/gad.271429.115;
RA Huang F., Saraf A., Florens L., Kusch T., Swanson S.K., Szerszen L.T.,
RA Li G., Dutta A., Washburn M.P., Abmayr S.M., Workman J.L.;
RT "The Enok acetyltransferase complex interacts with Elg1 and negatively
RT regulates PCNA unloading to promote the G1/S transition.";
RL Genes Dev. 30:1198-1210(2016).
CC -!- FUNCTION: Has an important role in DNA replication and in maintaining
CC genome integrity during replication stress (By similarity). Promotes
CC PCNA deubiquitination (By similarity). As component of the Elg1 RFC-
CC like complex, regulates the functions of the DNA polymerase
CC processivity factor PCNA by unloading it from DNA after replication
CC during the S phase of the cell cycle (PubMed:27198229). The PCNA-
CC unloading might be regulated via interaction with the Enok
CC acetyltransferase complex (PubMed:27198229). Might have a role in
CC restarting of stalled/regressed replication forks during replication
CC stress (By similarity). In the ovaries, has a role in nurse cell
CC endoreplication (PubMed:27198229). {ECO:0000250|UniProtKB:Q96QE3,
CC ECO:0000269|PubMed:27198229}.
CC -!- SUBUNIT: Component of a heteropentameric Elg1 RFC-like complex composed
CC of one large subunit (elg1) and four small subunits (RfC4, RfC38,
CC CG8142 and RfC3) (PubMed:27198229). As part of the complex, might
CC interact with the Enok complex, composed of enok, Br140, Eaf6 and Ing5
CC (PubMed:27198229). Within the Enok complex, interacts directly with
CC Br140 (PubMed:27198229). {ECO:0000269|PubMed:27198229}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:27198229}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=C {ECO:0000312|FlyBase:FBgn0036574};
CC IsoId=Q86BP6-1; Sequence=Displayed;
CC Name=D {ECO:0000312|FlyBase:FBgn0036574}; Synonyms=E
CC {ECO:0000312|FlyBase:FBgn0036574};
CC IsoId=Q86BP6-2; Sequence=VSP_061537;
CC -!- TISSUE SPECIFICITY: Expressed at higher levels in the germline nurse
CC cells than in the somatic follicle cells.
CC {ECO:0000269|PubMed:27198229}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in the ovaries, results
CC in lower DNA content in the nurse cells and defective development of
CC egg chambers (PubMed:27198229). Simultaneous knockdown of elg1 and
CC enok, results in relatively bigger and higher DNA content than single
CC elg1 knockdown (PubMed:27198229). Further, egg chambers in ~25% of the
CC ovarioles are able to develop beyond stage 9 with morphologically
CC normal nurse cells (PubMed:27198229). {ECO:0000269|PubMed:27198229}.
CC -!- SIMILARITY: Belongs to the ELG1 family. {ECO:0000305}.
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DR EMBL; AE014296; AAF49530.2; -; Genomic_DNA.
DR EMBL; AE014296; AAN11762.2; -; Genomic_DNA.
DR EMBL; AE014296; AFH04460.1; -; Genomic_DNA.
DR EMBL; AY118473; AAM49842.1; -; mRNA.
DR RefSeq; NP_001246789.1; NM_001259860.2.
DR RefSeq; NP_996102.1; NM_206380.2.
DR RefSeq; NP_996103.1; NM_206381.1.
DR IntAct; Q86BP6; 4.
DR STRING; 7227.FBpp0088654; -.
DR PaxDb; Q86BP6; -.
DR EnsemblMetazoa; FBtr0089713; FBpp0088654; FBgn0036574.
DR GeneID; 39770; -.
DR KEGG; dme:Dmel_CG16838; -.
DR UCSC; CG16838-RC; d. melanogaster.
DR CTD; 39770; -.
DR FlyBase; FBgn0036574; elg1.
DR VEuPathDB; VectorBase:FBgn0036574; -.
DR eggNOG; KOG1968; Eukaryota.
DR GeneTree; ENSGT00940000153469; -.
DR HOGENOM; CLU_269390_0_0_1; -.
DR InParanoid; Q86BP6; -.
DR OMA; QMYEESY; -.
DR OrthoDB; 455075at2759; -.
DR PhylomeDB; Q86BP6; -.
DR BioGRID-ORCS; 39770; 1 hit in 3 CRISPR screens.
DR GenomeRNAi; 39770; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036574; Expressed in egg cell and 20 other tissues.
DR ExpressionAtlas; Q86BP6; baseline and differential.
DR Genevisible; Q86BP6; DM.
DR GO; GO:0031391; C:Elg1 RFC-like complex; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0061860; F:DNA clamp unloader activity; IBA:GO_Central.
DR GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..1162
FT /note="Enhanced level of genomic instability 1"
FT /evidence="ECO:0000305"
FT /id="PRO_0000455976"
FT BINDING 703..710
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00499"
FT VAR_SEQ 1..56
FT /note="Missing (in isoform D)"
FT /evidence="ECO:0000305"
FT /id="VSP_061537"
FT CONFLICT 257..261
FT /note="APSSA -> VPSSS (in Ref. 3; AAM49842)"
FT /evidence="ECO:0000305"
FT CONFLICT 308
FT /note="L -> F (in Ref. 3; AAM49842)"
FT /evidence="ECO:0000305"
FT CONFLICT 495
FT /note="N -> S (in Ref. 3; AAM49842)"
FT /evidence="ECO:0000305"
FT CONFLICT 524
FT /note="A -> S (in Ref. 3; AAM49842)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1162 AA; 129367 MW; 9963817F75FEB29D CRC64;
MTDVNCLTSE HIAPTTTTAS PPGTPSKNAA TDPVEPTSPF VLRAPPSAKS KLILQNMEEV
LNSVKQKHRE KHKRKREEKR RAALMEDQKS TTEEVKANAK EKPQPLREKS SQENGLKNGR
RRSSPLKSST PVADNQSIMK HFAKAGKPEN VGVSPVSTAK PKPTTNVFEF MMNARNRSLG
VNEGGAESPA DQEIGSEAAT PTTKRKLLLQ EWNERKGGAK RRLADEARGE FIELQMEQRA
KRLRKMLTKT DAKEVTAPSS APTDPTVKRL RGRPRSRRIS SMDAQNQIVE SKPPVVKAKD
QDPGTEELLS KLSSPTKKRD SLLGYFPKVE SPKELAEKVI IAIETPPMET PKRRTLRRKS
QQETPIVAES TPSSRPRRSC VGKARYDYDL ETSPGKQQKA KPQKADESVE IIDLDNSNPA
ATPKKLAPLF VRQLPKPSPD PSVLKARQAF LQSGVPDKIR QEQIRQKNFD QMYEDSYEVF
PRLAHIGCES FGSKNGPLDI PFALRAEEEY SEVTKALPAN KRRAKASSIT SCLPADFTSS
KTLNKFNYAT LPQLENKRGF VKLWKNDFDR FPTFKCYNQM REKYRHFSAI DSAQDTQQMG
ESLVVTRRTR RSMEQNMAQN EEEAKPPPSA PNGELLFTEK YKPLLFEQVL VNLTPVQELR
EFLSSWSGNG GSNRNSQGMD DTFDMSNDSA SMGSSSNTMV LVGPSSSGKT NAVFTLANDM
NFNVLEINAG MKRTGKKLIQ ELQEATQSHQ IRKDGKAGGG SSQQLLQKLQ KSGLKAKAAA
VEPPSEVRKS LILIEDADIL FDNLDAGFTD AIYTLAASSK RPVIVVATDP NCAHLQRLMQ
QNIIHFQAPN ALNISRFLAV LSLMENCPIE LDELISVYLY NQQNLRKTLM ELQFYIQSGG
DSTRDRTGGG IKSPTKTSNS RLATVDGSRI HQRFFEFFTS PQNVQYRIPF PVDFSLLRLN
LPDLMASSAM LKEQQAAGGG SRTAAKRKSR SPKKAWLSSA TGQKSDGHSS SLATLASFYD
NISVASLMDS DCSDRLQWHL SEEIGHLLVE QALQTGLATK ECPYNLFDKP VQRFSICQHL
GNGVLRSDSA KSLDFEPALR SICRSEKERA GLERKSSRFY HYLRNHTVNV TSFTTDYFDT
ACSTFQSAAP SSEPTMEAEP KD
