ID   ELHB_APLCA              Reviewed;          54 AA.
AC   P11924;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Califin-B;
DE   Contains:
DE     RecName: Full=Califin-B large subunit;
DE   Contains:
DE     RecName: Full=Califin-B small subunit;
OS   Aplysia californica (California sea hare).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Heterobranchia; Euthyneura; Tectipleura; Aplysiida; Aplysioidea;
OC   Aplysiidae; Aplysia.
OX   NCBI_TaxID=6500;
RN   [1]
RP   PROTEIN SEQUENCE OF 1-36 AND 37-54, IDENTIFICATION BY MASS SPECTROMETRY,
RP   AND AMIDATION AT LEU-36.
RC   TISSUE=Atrial gland;
RX   PubMed=3753705; DOI=10.1016/s0021-9258(17)35984-7;
RA   Rothman B.S., Hawke D.H., Brown R.O., Lee T.D., Dehghan A.A., Shively J.E.,
RA   Mayeri E.;
RT   "Isolation and primary structure of the califins, three biologically active
RT   egg-laying hormone-like peptides from the atrial gland of Aplysia
RT   californica.";
RL   J. Biol. Chem. 261:1616-1623(1986).
RN   [2]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Atrial gland;
RX   PubMed=3711112; DOI=10.1016/s0021-9258(19)57481-6;
RA   Nagle G.T., Painter S.D., Blankenship J.E., Dixon J.D., Kurosky A.;
RT   "Evidence for the expression of three genes encoding homologous atrial
RT   gland peptides that cause egg laying in Aplysia.";
RL   J. Biol. Chem. 261:7853-7859(1986).
CC   -!- FUNCTION: Injected in sexually mature animals califin B excites LB and
CC       LC cells of the abdominal ganglion and cause egg-laying.
CC   -!- SUBUNIT: This protein consists of a large 36-residue subunit, bound by
CC       a single disulfide-bond to a small 18-residue subunit.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- MISCELLANEOUS: Califin B probably derives from polyprotein B, which is
CC       also the precursor for peptide B.
CC   -!- SIMILARITY: Belongs to the molluscan ELH family. {ECO:0000305}.
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DR   PIR; A01631; GOGABA.
DR   AlphaFoldDB; P11924; -.
DR   SMR; P11924; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR003424; ELH.
DR   Pfam; PF02323; ELH; 1.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond; Hormone;
KW   Neuropeptide; Secreted.
FT   PEPTIDE         1..36
FT                   /note="Califin-B large subunit"
FT                   /evidence="ECO:0000269|PubMed:3753705"
FT                   /id="PRO_0000001806"
FT   PEPTIDE         37..54
FT                   /note="Califin-B small subunit"
FT                   /id="PRO_0000001807"
FT   MOD_RES         36
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000269|PubMed:3753705"
FT   DISULFID        25..53
FT                   /note="Interchain (between large and small subunits)"
FT   NON_CONS        36..37
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   54 AA;  6057 MW;  C6B60521BC900914 CRC64;
     ISINQDLKAI TDMLLTEQIQ ARQRCLAALR QRLLDLDSDV SLFNGDLLPN GRCS