ELIP1_ARATH
ID ELIP1_ARATH Reviewed; 195 AA.
AC P93735;
DT 29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Early light-induced protein 1, chloroplastic {ECO:0000305};
DE Flags: Precursor;
GN Name=ELIP1 {ECO:0000303|PubMed:11706180};
GN Synonyms=ELIP {ECO:0000303|Ref.1}; OrderedLocusNames=At3g22840;
GN ORFNames=MWI23.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION BY LIGHT.
RC STRAIN=cv. Columbia;
RA Moscovici-Kadouri S., Chamovitz D.A.;
RT "Characterization of a cDNA encoding the early light-inducible protein
RT (ELIP) from Arabidopsis.";
RL (er) Plant Gene Register PGR97-155(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP SUBCELLULAR LOCATION, AND INDUCTION BY LIGHT.
RC STRAIN=cv. Columbia;
RX PubMed=10725357; DOI=10.1073/pnas.97.7.3741;
RA Heddad M., Adamska I.;
RT "Light stress-regulated two-helix proteins in Arabidopsis thaliana related
RT to the chlorophyll a/b-binding gene family.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:3741-3746(2000).
RN [7]
RP INDUCTION BY LIGHT.
RX PubMed=11706180; DOI=10.1104/pp.010270;
RA Harari-Steinberg O., Ohad I., Chamovitz D.A.;
RT "Dissection of the light signal transduction pathways regulating the two
RT early light-induced protein genes in Arabidopsis.";
RL Plant Physiol. 127:986-997(2001).
RN [8]
RP FUNCTION IN PHOTOPROTECTION, AND INDUCTION BY LIGHT.
RC STRAIN=cv. Columbia;
RX PubMed=12676998; DOI=10.1073/pnas.0736939100;
RA Hutin C., Nussaume L., Moise N., Moya I., Kloppstech K., Havaux M.;
RT "Early light-induced proteins protect Arabidopsis from photooxidative
RT stress.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:4921-4926(2003).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, AND INDUCTION BY
RP LIGHT.
RC STRAIN=cv. Columbia;
RX PubMed=16028115; DOI=10.1007/s11103-005-4090-1;
RA Casazza A.P., Rossini S., Rosso M.G., Soave C.;
RT "Mutational and expression analysis of ELIP1 and ELIP2 in Arabidopsis
RT thaliana.";
RL Plant Mol. Biol. 58:41-51(2005).
RN [10]
RP SUBCELLULAR LOCATION, AND TRANSIT PEPTIDE CLEAVAGE SITE.
RX PubMed=16806197; DOI=10.1016/j.febslet.2006.06.018;
RA Bhushan S., Kuhn C., Berglund A.K., Roth C., Glaser E.;
RT "The role of the N-terminal domain of chloroplast targeting peptides in
RT organellar protein import and miss-sorting.";
RL FEBS Lett. 580:3966-3972(2006).
RN [11]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=16778010; DOI=10.1104/pp.106.083055;
RA Rossini S., Casazza A.P., Engelmann E.C., Havaux M., Jennings R.C.,
RA Soave C.;
RT "Suppression of both ELIP1 and ELIP2 in Arabidopsis does not affect
RT tolerance to photoinhibition and photooxidative stress.";
RL Plant Physiol. 141:1264-1273(2006).
RN [12]
RP INDUCTION BY LIGHT, AND SUBCELLULAR LOCATION.
RX PubMed=16829586; DOI=10.1104/pp.106.081489;
RA Heddad M., Noren H., Reiser V., Dunaeva M., Andersson B., Adamska I.;
RT "Differential expression and localization of early light-induced proteins
RT in Arabidopsis.";
RL Plant Physiol. 142:75-87(2006).
RN [13]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=21299564; DOI=10.1111/j.1469-8137.2010.03637.x;
RA Rizza A., Boccaccini A., Lopez-Vidriero I., Costantino P., Vittorioso P.;
RT "Inactivation of the ELIP1 and ELIP2 genes affects Arabidopsis seed
RT germination.";
RL New Phytol. 190:896-905(2011).
CC -!- FUNCTION: Prevents excess accumulation of free chlorophyll by
CC inhibiting the entire chlorophyll biosynthesis pathway (e.g. 5-
CC aminolevulinate synthesis and Mg-protoporphyrin IX chelatase activity),
CC and hence prevent photooxidative stress (By similarity). Probably
CC involved in the integration of pigments into the mature light-
CC harvesting pigment-protein complexes. Light-harvesting chlorophyll
CC (LHC) a/b-binding protein required to ensure a high rate of chlorophyll
CC accumulation during deetiolation in continuous high light. Involved in
CC seed germination. May fulfill a photoprotective functions.
CC {ECO:0000250, ECO:0000269|PubMed:12676998, ECO:0000269|PubMed:16028115,
CC ECO:0000269|PubMed:16778010, ECO:0000269|PubMed:21299564}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:10725357, ECO:0000269|PubMed:16806197,
CC ECO:0000269|PubMed:16829586}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Associated with both photosystems I and II (By
CC similarity). Coisolates equally with monomeric (mLhcb) and trimeric
CC (tLhcb) populations of the major LHC from photosystem II (PSII) in
CC response to 2 hours light stress. {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Appears transiently during greening of etiolated
CC seedlings and disappears before chloroplast development is completed.
CC {ECO:0000269|PubMed:16028115}.
CC -!- INDUCTION: Levels increase linearly with increasing light intensities
CC and correlate with the degree of photoinactivation and photodamage of
CC PSII reaction centers (at protein level). Induced by high-intensity
CC light, at both cold and warm temperatures (e.g. 4 and 22 degrees
CC Celsius) (at protein level); quickly and transiently induced during
CC deetiolation, and accumulates in green seedlings following increases in
CC light intensity. Induced by UV-A, red, far-red and blue lights
CC illumination in a phytochrome A and phytochrome B-dependent manner;
CC this induction is promoted by HY5. The COP9 signalosome is involved in
CC dark-mediated repression. Accumulates upon heat shock. Transcript
CC levels follow a circadian cycle, with highest levels 2 h after light,
CC without protein accumulation. In light stress-preadapted or senescent
CC leaves exposed to light stress there is a lack of correlation between
CC transcript and protein accumulation; transcripts accumulate in red and
CC yellow leaves exposed to high light, but not proteins.
CC {ECO:0000269|PubMed:10725357, ECO:0000269|PubMed:11706180,
CC ECO:0000269|PubMed:12676998, ECO:0000269|PubMed:16028115,
CC ECO:0000269|PubMed:16829586, ECO:0000269|Ref.1}.
CC -!- DISRUPTION PHENOTYPE: Reduced greening during deetiolation in
CC continuous high light, with a reduced ratio between chlorophylls a and
CC especially at the highest irradiances. Slight reduction in the rate of
CC chlorophyll accumulation during greening at moderate light intensities,
CC and lower zeaxanthin accumulation in high light conditions. Normal
CC sensitivity to photoinhibition and photooxidation. Impaired seed
CC germination, especially in high light conditions and at warm to hot
CC temperatures (greater than 22 degrees Celsius).
CC {ECO:0000269|PubMed:16028115, ECO:0000269|PubMed:16778010,
CC ECO:0000269|PubMed:21299564}.
CC -!- SIMILARITY: Belongs to the ELIP/psbS family. {ECO:0000305}.
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DR EMBL; U89014; AAB88391.1; -; mRNA.
DR EMBL; AB022223; BAB01259.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76682.1; -; Genomic_DNA.
DR EMBL; AY057560; AAL09799.1; -; mRNA.
DR EMBL; AY075672; AAL77679.1; -; mRNA.
DR EMBL; AY097423; AAM19939.1; -; mRNA.
DR EMBL; AY085317; AAM62548.1; -; mRNA.
DR RefSeq; NP_188923.1; NM_113183.4.
DR AlphaFoldDB; P93735; -.
DR SMR; P93735; -.
DR BioGRID; 7187; 23.
DR IntAct; P93735; 23.
DR STRING; 3702.AT3G22840.1; -.
DR PaxDb; P93735; -.
DR PRIDE; P93735; -.
DR ProteomicsDB; 222319; -.
DR EnsemblPlants; AT3G22840.1; AT3G22840.1; AT3G22840.
DR GeneID; 821855; -.
DR Gramene; AT3G22840.1; AT3G22840.1; AT3G22840.
DR KEGG; ath:AT3G22840; -.
DR Araport; AT3G22840; -.
DR TAIR; locus:2094354; AT3G22840.
DR eggNOG; ENOG502RZBJ; Eukaryota.
DR HOGENOM; CLU_099630_0_0_1; -.
DR InParanoid; P93735; -.
DR OMA; LVFTEYL; -.
DR OrthoDB; 1510113at2759; -.
DR PhylomeDB; P93735; -.
DR PRO; PR:P93735; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; P93735; baseline and differential.
DR Genevisible; P93735; AT.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009522; C:photosystem I; IEA:UniProtKB-KW.
DR GO; GO:0009523; C:photosystem II; IEA:UniProtKB-KW.
DR GO; GO:0071483; P:cellular response to blue light; IEP:UniProtKB.
DR GO; GO:0071490; P:cellular response to far red light; IEP:UniProtKB.
DR GO; GO:0034605; P:cellular response to heat; IEP:UniProtKB.
DR GO; GO:0071486; P:cellular response to high light intensity; IEP:UniProtKB.
DR GO; GO:0071491; P:cellular response to red light; IEP:UniProtKB.
DR GO; GO:0071492; P:cellular response to UV-A; IEP:UniProtKB.
DR GO; GO:0010117; P:photoprotection; IMP:UniProtKB.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR GO; GO:0010030; P:positive regulation of seed germination; IMP:UniProtKB.
DR GO; GO:0010380; P:regulation of chlorophyll biosynthetic process; ISS:UniProtKB.
DR GO; GO:0009409; P:response to cold; IEP:TAIR.
DR InterPro; IPR022796; Chloroa_b-bind.
DR Pfam; PF00504; Chloroa_b-bind; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Photosynthesis; Photosystem I; Photosystem II;
KW Plastid; Reference proteome; Thylakoid; Transit peptide; Transmembrane;
KW Transmembrane helix.
FT TRANSIT 1..46
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:16806197"
FT CHAIN 47..195
FT /note="Early light-induced protein 1, chloroplastic"
FT /id="PRO_0000422364"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..195
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 46..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 65..81
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 195 AA; 20325 MW; 519AAB7D271D5551 CRC64;
MATASFNMQS VFAGGLTTRK INTNKLFSAG SFPNLKRNYP VGVRCMAEGG PTNEDSSPAP
STSAAQPLPK SPSPPPPMKP KVSTKFSDLL AFSGPAPERI NGRLAMVGFV AALAVELSKG
ENVLAQISDG GVSWFLGTTA ILTLASLVPL FKGISVESKS KGIMTSDAEL WNGRFAMLGL
VALAFTEFVK GGTLV
