ELIP2_ARATH
ID ELIP2_ARATH Reviewed; 193 AA.
AC Q94K66; O23325; Q42127; Q8L8U1; Q9SYX0;
DT 29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Early light-induced protein 2, chloroplastic {ECO:0000305};
DE Flags: Precursor;
GN Name=ELIP2 {ECO:0000303|PubMed:11473579}; OrderedLocusNames=At4g14690;
GN ORFNames=dl3385w, FCAALL.232;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 4-193.
RX PubMed=10366881; DOI=10.1016/s1360-1385(99)01419-3;
RA Jansson S.;
RT "A guide to the Lhc genes and their relatives in Arabidopsis.";
RL Trends Plant Sci. 4:236-240(1999).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 115-193.
RC STRAIN=cv. Columbia; TISSUE=Seedling;
RA Raynal M., Grellet F., Laudie M., Meyer Y., Cooke R., Delseny M.;
RT "The Arabidopsis thaliana transcribed genome: the GDR cDNA program.";
RL Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP SUBCELLULAR LOCATION, AND INDUCTION BY LIGHT.
RC STRAIN=cv. Columbia;
RX PubMed=10725357; DOI=10.1073/pnas.97.7.3741;
RA Heddad M., Adamska I.;
RT "Light stress-regulated two-helix proteins in Arabidopsis thaliana related
RT to the chlorophyll a/b-binding gene family.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:3741-3746(2000).
RN [9]
RP INDUCTION BY LIGHT.
RX PubMed=11473579; DOI=10.1046/j.1365-2443.2001.00446.x;
RA Kimura M., Yoshizumi T., Manabe K., Yamamoto Y.Y., Matsui M.;
RT "Arabidopsis transcriptional regulation by light stress via hydrogen
RT peroxide-dependent and -independent pathways.";
RL Genes Cells 6:607-617(2001).
RN [10]
RP INDUCTION BY LIGHT.
RX PubMed=11706180; DOI=10.1104/pp.010270;
RA Harari-Steinberg O., Ohad I., Chamovitz D.A.;
RT "Dissection of the light signal transduction pathways regulating the two
RT early light-induced protein genes in Arabidopsis.";
RL Plant Physiol. 127:986-997(2001).
RN [11]
RP FUNCTION IN PHOTOPROTECTION, AND INDUCTION BY LIGHT.
RC STRAIN=cv. Columbia;
RX PubMed=12676998; DOI=10.1073/pnas.0736939100;
RA Hutin C., Nussaume L., Moise N., Moya I., Kloppstech K., Havaux M.;
RT "Early light-induced proteins protect Arabidopsis from photooxidative
RT stress.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:4921-4926(2003).
RN [12]
RP FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, AND INDUCTION BY
RP LIGHT.
RC STRAIN=cv. Columbia;
RX PubMed=16028115; DOI=10.1007/s11103-005-4090-1;
RA Casazza A.P., Rossini S., Rosso M.G., Soave C.;
RT "Mutational and expression analysis of ELIP1 and ELIP2 in Arabidopsis
RT thaliana.";
RL Plant Mol. Biol. 58:41-51(2005).
RN [13]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=16778010; DOI=10.1104/pp.106.083055;
RA Rossini S., Casazza A.P., Engelmann E.C., Havaux M., Jennings R.C.,
RA Soave C.;
RT "Suppression of both ELIP1 and ELIP2 in Arabidopsis does not affect
RT tolerance to photoinhibition and photooxidative stress.";
RL Plant Physiol. 141:1264-1273(2006).
RN [14]
RP INDUCTION BY LIGHT, AND SUBCELLULAR LOCATION.
RX PubMed=16829586; DOI=10.1104/pp.106.081489;
RA Heddad M., Noren H., Reiser V., Dunaeva M., Andersson B., Adamska I.;
RT "Differential expression and localization of early light-induced proteins
RT in Arabidopsis.";
RL Plant Physiol. 142:75-87(2006).
RN [15]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=17553115; DOI=10.1111/j.1365-313x.2007.03090.x;
RA Tzvetkova-Chevolleau T., Franck F., Alawady A.E., Dall'Osto L.,
RA Carriere F., Bassi R., Grimm B., Nussaume L., Havaux M.;
RT "The light stress-induced protein ELIP2 is a regulator of chlorophyll
RT synthesis in Arabidopsis thaliana.";
RL Plant J. 50:795-809(2007).
RN [16]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=21299564; DOI=10.1111/j.1469-8137.2010.03637.x;
RA Rizza A., Boccaccini A., Lopez-Vidriero I., Costantino P., Vittorioso P.;
RT "Inactivation of the ELIP1 and ELIP2 genes affects Arabidopsis seed
RT germination.";
RL New Phytol. 190:896-905(2011).
CC -!- FUNCTION: Probably involved in the integration of pigments into the
CC mature light-harvesting pigment-protein complexes. Light-harvesting
CC chlorophyll (LHC) a/b-binding protein required to ensure a high rate of
CC chlorophyll accumulation during deetiolation in continuous high light.
CC Involved in seed germination. May fulfill a photoprotective functions.
CC Prevents excess accumulation of free chlorophyll by inhibiting the
CC entire chlorophyll biosynthesis pathway (e.g. 5-aminolevulinate
CC synthesis and Mg-protoporphyrin IX chelatase activity), and hence
CC prevent photooxidative stress. {ECO:0000269|PubMed:12676998,
CC ECO:0000269|PubMed:16028115, ECO:0000269|PubMed:16778010,
CC ECO:0000269|PubMed:17553115, ECO:0000269|PubMed:21299564}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:10725357, ECO:0000269|PubMed:16829586}; Multi-pass
CC membrane protein {ECO:0000255}. Note=Associated with both photosystems
CC I and II (By similarity). Coisolates equally with monomeric (mLhcb) and
CC trimeric (tLhcb) populations of the major LHC from photosystem II
CC (PSII) in response to 2 hours light stress. {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Appears transiently during greening of etiolated
CC seedlings and disappears before chloroplast development is completed.
CC {ECO:0000269|PubMed:16028115}.
CC -!- INDUCTION: By high-intensity light, mostly at cold temperature (e.g. 4
CC degrees Celsius) (at protein level). This high-light-mediated
CC accumulation is inhibited by okadaic acid. A stepwise accumulation is
CC induced when 40 percents of PSII reaction centers become photodamaged.
CC Induced by UV-A, red, far-red and blue lights illumination in a
CC phytochrome A and phytochrome B-dependent manner. The COP9 signalosome
CC is involved in dark-mediated repression. Accumulates upon heat shock
CC but repressed at continuous high temperatures (). Transcript levels
CC follow a circadian cycle, with highest levels 2 h after light, without
CC protein accumulation. In light stress-preadapted or senescent leaves
CC exposed to light stress there is a lack of correlation between
CC transcript and protein accumulation; transcripts accumulate in yellow
CC leaves exposed to high light, but not proteins.
CC {ECO:0000269|PubMed:10725357, ECO:0000269|PubMed:11473579,
CC ECO:0000269|PubMed:11706180, ECO:0000269|PubMed:12676998,
CC ECO:0000269|PubMed:16028115, ECO:0000269|PubMed:16829586}.
CC -!- DISRUPTION PHENOTYPE: Reduced greening during deetiolation in
CC continuous high light, with a reduced ratio between chlorophylls a and
CC especially at the highest irradiances. Slight reduction in the rate of
CC chlorophyll accumulation during greening at moderate light intensities,
CC and lower zeaxanthin accumulation in high light conditions. Normal
CC sensitivity to photoinhibition and photooxidation. Impaired seed
CC germination, especially at hot temperatures (30 degrees Celsius).
CC {ECO:0000269|PubMed:16028115, ECO:0000269|PubMed:16778010,
CC ECO:0000269|PubMed:21299564}.
CC -!- SIMILARITY: Belongs to the ELIP/psbS family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD28779.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB10248.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78511.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; Z97336; CAB10248.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161539; CAB78511.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE83475.1; -; Genomic_DNA.
DR EMBL; AF370266; AAK44081.1; -; mRNA.
DR EMBL; AY063083; AAL34257.1; -; mRNA.
DR EMBL; AY088811; AAM67121.1; -; mRNA.
DR EMBL; AF134132; AAD28779.1; ALT_INIT; mRNA.
DR EMBL; Z26549; CAA81320.1; -; mRNA.
DR PIR; F71409; F71409.
DR PIR; T52309; T52309.
DR RefSeq; NP_567438.1; NM_117551.3.
DR AlphaFoldDB; Q94K66; -.
DR SMR; Q94K66; -.
DR BioGRID; 12416; 23.
DR IntAct; Q94K66; 23.
DR STRING; 3702.AT4G14690.1; -.
DR PaxDb; Q94K66; -.
DR PRIDE; Q94K66; -.
DR ProteomicsDB; 221940; -.
DR EnsemblPlants; AT4G14690.1; AT4G14690.1; AT4G14690.
DR GeneID; 827119; -.
DR Gramene; AT4G14690.1; AT4G14690.1; AT4G14690.
DR KEGG; ath:AT4G14690; -.
DR Araport; AT4G14690; -.
DR TAIR; locus:2130085; AT4G14690.
DR eggNOG; ENOG502RZBJ; Eukaryota.
DR HOGENOM; CLU_099630_0_0_1; -.
DR InParanoid; Q94K66; -.
DR OMA; NTNQLFF; -.
DR OrthoDB; 1510113at2759; -.
DR PhylomeDB; Q94K66; -.
DR PRO; PR:Q94K66; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q94K66; baseline and differential.
DR Genevisible; Q94K66; AT.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009522; C:photosystem I; IEA:UniProtKB-KW.
DR GO; GO:0009523; C:photosystem II; IEA:UniProtKB-KW.
DR GO; GO:0071483; P:cellular response to blue light; IEP:UniProtKB.
DR GO; GO:0071490; P:cellular response to far red light; IEP:UniProtKB.
DR GO; GO:0034605; P:cellular response to heat; IEP:UniProtKB.
DR GO; GO:0071486; P:cellular response to high light intensity; IEP:UniProtKB.
DR GO; GO:0071491; P:cellular response to red light; IEP:UniProtKB.
DR GO; GO:0071492; P:cellular response to UV-A; IEP:UniProtKB.
DR GO; GO:0010117; P:photoprotection; IMP:UniProtKB.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR GO; GO:0010030; P:positive regulation of seed germination; IMP:UniProtKB.
DR GO; GO:0010380; P:regulation of chlorophyll biosynthetic process; IMP:TAIR.
DR GO; GO:0010218; P:response to far red light; IEP:TAIR.
DR GO; GO:0010114; P:response to red light; IEP:TAIR.
DR GO; GO:0010224; P:response to UV-B; IEP:TAIR.
DR InterPro; IPR022796; Chloroa_b-bind.
DR Pfam; PF00504; Chloroa_b-bind; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Photosynthesis; Photosystem I; Photosystem II;
KW Plastid; Reference proteome; Thylakoid; Transit peptide; Transmembrane;
KW Transmembrane helix.
FT TRANSIT 1..43
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 44..193
FT /note="Early light-induced protein 2, chloroplastic"
FT /id="PRO_0000422365"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 46..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 35
FT /note="I -> N (in Ref. 5; AAM67121)"
FT /evidence="ECO:0000305"
FT CONFLICT 64
FT /note="P -> Q (in Ref. 5; AAM67121)"
FT /evidence="ECO:0000305"
FT CONFLICT 113
FT /note="M -> V (in Ref. 5; AAM67121)"
FT /evidence="ECO:0000305"
FT CONFLICT 193
FT /note="V -> VLN (in Ref. 7; CAA81320)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 193 AA; 20345 MW; 1D513A798E5B1F75 CRC64;
MATASFNMQS VFAAPSGVLT TRNIRNTNQL FFKRIAPVGV RCMAQGDPIK EDPSVPSTST
SATPPQMPQS PPPPVSKPKV STKFGDLLAF SGPAPERING RLAMVGFVAA IAMELSKGEN
VFAQISDGGV GWFLGTTALL TLASMVPLFK GIRAEAKSKG FMTSDAELWN GRFAMLGLVA
LAFTEYVTGG TLV
