ELL3_MOUSE
ID ELL3_MOUSE Reviewed; 395 AA.
AC Q80VR2; A2ARQ1;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=RNA polymerase II elongation factor ELL3;
GN Name=Ell3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|EMBL:AAH45151.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Olfactory epithelium {ECO:0000312|EMBL:AAH45151.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH AFF4.
RX PubMed=20159561; DOI=10.1016/j.molcel.2010.01.026;
RA Lin C., Smith E.R., Takahashi H., Lai K.C., Martin-Brown S., Florens L.,
RA Washburn M.P., Conaway J.W., Conaway R.C., Shilatifard A.;
RT "AFF4, a component of the ELL/P-TEFb elongation complex and a shared
RT subunit of MLL chimeras, can link transcription elongation to leukemia.";
RL Mol. Cell 37:429-437(2010).
RN [4]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=22768269; DOI=10.1371/journal.pone.0040293;
RA Ahn H.J., Cha Y., Moon S.H., Jung J.E., Park K.S.;
RT "Ell3 enhances differentiation of mouse embryonic stem cells by regulating
RT epithelial-mesenchymal transition and apoptosis.";
RL PLoS ONE 7:E40293-E40293(2012).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH AFF4.
RX PubMed=23273992; DOI=10.1016/j.cell.2012.12.015;
RA Lin C., Garruss A.S., Luo Z., Guo F., Shilatifard A.;
RT "The RNA Pol II elongation factor Ell3 marks enhancers in ES cells and
RT primes future gene activation.";
RL Cell 152:144-156(2013).
CC -!- FUNCTION: Enhancer-binding elongation factor that specifically binds
CC enhancers in embryonic stem cells (ES cells), marks them, and is
CC required for their future activation during stem cell specification.
CC Elongation factor component of the super elongation complex (SEC), a
CC complex required to increase the catalytic rate of RNA polymerase II
CC transcription by suppressing transient pausing by the polymerase at
CC multiple sites along the DNA. Component of the little elongation
CC complex (LEC), a complex required to regulate small nuclear RNA (snRNA)
CC gene transcription by RNA polymerase II and III. Does not only bind to
CC enhancer regions of active genes, but also marks the enhancers that are
CC in a poised or inactive state in ES cells and is required for
CC establishing proper RNA polymerase II occupancy at developmentally
CC regulated genes in a cohesin-dependent manner. Probably required for
CC priming developmentally regulated genes for later recruitment of the
CC super elongation complex (SEC), for transcriptional activation during
CC differentiation. Required for recruitment of P-TEFb within SEC during
CC differentiation. Probably preloaded on germ cell chromatin, suggesting
CC that it may prime gene activation by marking enhancers as early as in
CC the germ cells. Promoting epithelial-mesenchymal transition (EMT).
CC {ECO:0000269|PubMed:22768269, ECO:0000269|PubMed:23273992}.
CC -!- SUBUNIT: Component of the little elongation complex (LEC), at least
CC composed of ELL (ELL, ELL2 or ELL3), ZC3H8, ICE1 and ICE2 (By
CC similarity). Component of the super elongation complex (SEC), at least
CC composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF (AFF1 or AFF4), the P-TEFb
CC complex and ELL (ELL, ELL2 or ELL3). Interacts with AFF4. {ECO:0000250,
CC ECO:0000269|PubMed:20159561, ECO:0000269|PubMed:23273992}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23273992}.
CC -!- TISSUE SPECIFICITY: Actively expressed in embryonic stem cells (ES
CC cells), while it is weakly expressed in differentiated cells.
CC {ECO:0000269|PubMed:22768269}.
CC -!- SIMILARITY: Belongs to the ELL/occludin family. {ECO:0000305}.
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DR EMBL; AL845466; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC045151; AAH45151.1; -; mRNA.
DR CCDS; CCDS16644.1; -.
DR RefSeq; NP_666085.2; NM_145973.2.
DR AlphaFoldDB; Q80VR2; -.
DR SMR; Q80VR2; -.
DR BioGRID; 234639; 2.
DR STRING; 10090.ENSMUSP00000028679; -.
DR PhosphoSitePlus; Q80VR2; -.
DR MaxQB; Q80VR2; -.
DR PaxDb; Q80VR2; -.
DR PeptideAtlas; Q80VR2; -.
DR PRIDE; Q80VR2; -.
DR ProteomicsDB; 277782; -.
DR Antibodypedia; 24110; 128 antibodies from 21 providers.
DR DNASU; 269344; -.
DR Ensembl; ENSMUST00000028679; ENSMUSP00000028679; ENSMUSG00000027246.
DR Ensembl; ENSMUST00000116432; ENSMUSP00000112133; ENSMUSG00000027246.
DR GeneID; 269344; -.
DR KEGG; mmu:269344; -.
DR UCSC; uc008lzc.1; mouse.
DR CTD; 80237; -.
DR MGI; MGI:2673679; Ell3.
DR VEuPathDB; HostDB:ENSMUSG00000027246; -.
DR eggNOG; KOG4796; Eukaryota.
DR GeneTree; ENSGT00940000161615; -.
DR HOGENOM; CLU_692530_0_0_1; -.
DR InParanoid; Q80VR2; -.
DR OMA; RLHCLGP; -.
DR OrthoDB; 335949at2759; -.
DR PhylomeDB; Q80VR2; -.
DR TreeFam; TF337345; -.
DR Reactome; R-MMU-6807505; RNA polymerase II transcribes snRNA genes.
DR BioGRID-ORCS; 269344; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Ell3; mouse.
DR PRO; PR:Q80VR2; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q80VR2; protein.
DR Bgee; ENSMUSG00000027246; Expressed in spleen and 63 other tissues.
DR Genevisible; Q80VR2; MM.
DR GO; GO:0030054; C:cell junction; ISO:MGI.
DR GO; GO:0005694; C:chromosome; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0008023; C:transcription elongation factor complex; ISS:UniProtKB.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0006354; P:DNA-templated transcription, elongation; ISO:MGI.
DR GO; GO:0042771; P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; IMP:MGI.
DR GO; GO:1902166; P:negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; IMP:MGI.
DR GO; GO:1901797; P:negative regulation of signal transduction by p53 class mediator; IDA:MGI.
DR GO; GO:0061351; P:neural precursor cell proliferation; IDA:MGI.
DR GO; GO:0032786; P:positive regulation of DNA-templated transcription, elongation; ISO:MGI.
DR GO; GO:2000179; P:positive regulation of neural precursor cell proliferation; IDA:MGI.
DR GO; GO:0050769; P:positive regulation of neurogenesis; IDA:MGI.
DR GO; GO:2000648; P:positive regulation of stem cell proliferation; IDA:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR GO; GO:0010717; P:regulation of epithelial to mesenchymal transition; IMP:UniProtKB.
DR GO; GO:0072331; P:signal transduction by p53 class mediator; IDA:MGI.
DR GO; GO:0042795; P:snRNA transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0048863; P:stem cell differentiation; IMP:UniProtKB.
DR GO; GO:0072089; P:stem cell proliferation; IDA:MGI.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; ISO:MGI.
DR InterPro; IPR031176; ELL/occludin.
DR InterPro; IPR031175; ELL3.
DR InterPro; IPR019464; ELL_N.
DR InterPro; IPR010844; Occludin_ELL.
DR PANTHER; PTHR23288; PTHR23288; 1.
DR PANTHER; PTHR23288:SF18; PTHR23288:SF18; 1.
DR Pfam; PF10390; ELL; 1.
DR Pfam; PF07303; Occludin_ELL; 1.
DR PROSITE; PS51980; OCEL; 1.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..395
FT /note="RNA polymerase II elongation factor ELL3"
FT /id="PRO_0000146737"
FT DOMAIN 283..393
FT /note="OCEL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01324"
FT REGION 129..177
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 189..281
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 130..152
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 203..225
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 245..261
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 242
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5XFX8"
SQ SEQUENCE 395 AA; 44762 MW; 9297E95C5AD222EA CRC64;
MEGTQEALSG KMRLLFTPAA RTSLLMLRLN EAALRALQEC QQQQVRPVIA FQGHRGYLRF
PGPGWSCLFS FIVSQCGQEG TNGGLDLVYQ RLGRSGPNCL HCLGSLRERL TIWAAMDTIP
APLLAQEHLT EGTRESESWQ DTGDEPEGHP QLAPDEVSDP LASHHEQSLP GSSSEPMAQW
EMRNHTYLPS REPDQSLLSP ASQKRLDKKR SAPITTEEPE EKRLRALPLA SSPLQGLANQ
DSQEGEDWGQ DEDEEGDEDG DSRLEQSLSA PSASESPSPE EVPDYLLQYR AIHSTEQQQA
YEQDFETDYA EYRILHARVG AASQRFTELG AEIKRLQRGT PEHKVLEDKI VQEYKKFRKR
YPSYREEKHR CEYLHQKLSH IKGLILEFEE KNRGS
