ELLI1_CAEEL
ID ELLI1_CAEEL Reviewed; 277 AA.
AC D3YT16; D3YT17; D3YT18; Q19634;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2011, sequence version 2.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Enlarged germline granules protein 1 {ECO:0000303|PubMed:28182654};
GN Name=elli-1 {ECO:0000303|PubMed:28182654, ECO:0000312|WormBase:F20C5.3b};
GN ORFNames=F20C5.3 {ECO:0000312|WormBase:F20C5.3b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP DISRUPTION PHENOTYPE, AND MUTAGENESIS OF 184-LYS--PHE-277.
RX PubMed=28182654; DOI=10.1371/journal.pgen.1006611;
RA Andralojc K.M., Campbell A.C., Kelly A.L., Terrey M., Tanner P.C.,
RA Gans I.M., Senter-Zapata M.J., Khokhar E.S., Updike D.L.;
RT "ELLI-1, a novel germline protein, modulates RNAi activity and P-granule
RT accumulation in Caenorhabditis elegans.";
RL PLoS Genet. 13:E1006611-E1006611(2017).
CC -!- FUNCTION: Plays a role in modulating RNAi activity and P-granule
CC accumulation in the germline (PubMed:28182654). May function in
CC association with CSR-1 complex components such as drh-3 to regulate RNA
CC transcript accumulation in germ cells (PubMed:28182654).
CC {ECO:0000269|PubMed:28182654}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:28182654}.
CC Cytoplasmic granule {ECO:0000269|PubMed:28182654}. Note=Mainly
CC localizes to the rachis, which is the central core of the cytoplasm
CC connecting developing oocytes in the syncytial gonad (PubMed:28182654).
CC Within the rachis, partially co-localizes with the P-body component
CC cgh-1 (PubMed:28182654). Localizes to foci next to P-granules, but does
CC not co-localize with P-granules (PubMed:28182654).
CC {ECO:0000269|PubMed:28182654}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=b {ECO:0000312|WormBase:F20C5.3b};
CC IsoId=D3YT16-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:F20C5.3a};
CC IsoId=D3YT16-2; Sequence=VSP_060477;
CC Name=c {ECO:0000312|WormBase:F20C5.3c};
CC IsoId=D3YT16-3; Sequence=VSP_060476;
CC Name=d {ECO:0000312|WormBase:F20C5.3d};
CC IsoId=D3YT16-4; Sequence=VSP_060475;
CC -!- TISSUE SPECIFICITY: Highly expressed in the germline.
CC {ECO:0000269|PubMed:28182654}.
CC -!- DEVELOPMENTAL STAGE: First expressed in primordial germ cells between
CC the comma to 2-fold stage of embryogenesis.
CC {ECO:0000269|PubMed:28182654}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in embryonic
CC lethality in 5-8% of animals (PubMed:28182654). Surviving animals have
CC enlarged P-granules in the germline (PubMed:28182654).
CC {ECO:0000269|PubMed:28182654}.
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DR EMBL; BX284604; CAA92296.2; -; Genomic_DNA.
DR EMBL; BX284604; CBK19442.2; -; Genomic_DNA.
DR EMBL; BX284604; CBK19443.2; -; Genomic_DNA.
DR EMBL; BX284604; CBK19444.1; -; Genomic_DNA.
DR PIR; T21135; T21135.
DR RefSeq; NP_001255325.1; NM_001268396.1. [D3YT16-2]
DR RefSeq; NP_001255326.1; NM_001268397.1. [D3YT16-1]
DR RefSeq; NP_001255327.1; NM_001268398.1. [D3YT16-3]
DR RefSeq; NP_001255328.1; NM_001268399.1. [D3YT16-4]
DR AlphaFoldDB; D3YT16; -.
DR STRING; 6239.F20C5.3b; -.
DR PaxDb; D3YT16; -.
DR PeptideAtlas; D3YT16; -.
DR EnsemblMetazoa; F20C5.3a.1; F20C5.3a.1; WBGene00008968. [D3YT16-2]
DR EnsemblMetazoa; F20C5.3b.1; F20C5.3b.1; WBGene00008968. [D3YT16-1]
DR EnsemblMetazoa; F20C5.3c.1; F20C5.3c.1; WBGene00008968. [D3YT16-3]
DR EnsemblMetazoa; F20C5.3d.1; F20C5.3d.1; WBGene00008968. [D3YT16-4]
DR GeneID; 184716; -.
DR KEGG; cel:CELE_F20C5.3; -.
DR UCSC; F20C5.3; c. elegans.
DR CTD; 184716; -.
DR WormBase; F20C5.3a; CE35500; WBGene00008968; elli-1. [D3YT16-2]
DR WormBase; F20C5.3b; CE03240; WBGene00008968; elli-1. [D3YT16-1]
DR WormBase; F20C5.3c; CE45422; WBGene00008968; elli-1. [D3YT16-3]
DR WormBase; F20C5.3d; CE44699; WBGene00008968; elli-1. [D3YT16-4]
DR eggNOG; ENOG502TJ51; Eukaryota.
DR HOGENOM; CLU_105167_0_0_1; -.
DR InParanoid; D3YT16; -.
DR OMA; KMWIFES; -.
DR OrthoDB; 1871957at2759; -.
DR PRO; PR:D3YT16; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00008968; Expressed in adult organism and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:WormBase.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:1903863; P:P granule assembly; IMP:WormBase.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Reference proteome.
FT CHAIN 1..277
FT /note="Enlarged germline granules protein 1"
FT /id="PRO_0000448949"
FT REGION 95..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 95..143
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..126
FT /note="Missing (in isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_060475"
FT VAR_SEQ 1..68
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_060476"
FT VAR_SEQ 67..114
FT /note="VKMYSEPARQTTWKEKLSQLNHENMSYNAHNKRSSRQRGNSYRLPQQN ->
FT D (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_060477"
FT MUTAGEN 184..277
FT /note="Missing: In sam3 and sam6; enlarged P-granules in
FT the germline and accumulation of RNA transcripts in the
FT central rachis and around the nuclear periphery of germ
FT cells. Enhances the sterility and RNAi defective phenotypes
FT of the drh-3 (sam27) mutant."
FT /evidence="ECO:0000269|PubMed:28182654"
SQ SEQUENCE 277 AA; 31919 MW; 2686934D6407AB65 CRC64;
MSNGKRGRKA KPTNKEKDMI FTPGCVDCHM NHLPFQCPRP VMTKKLPEWE KKSIGMGKLK
NPLDSKVKMY SEPARQTTWK EKLSQLNHEN MSYNAHNKRS SRQRGNSYRL PQQNIPPPSH
HQSSQVMRGF QNAPFQMQPH YNNERKPQSR RNDTMRASKS AYAAATAPVK KHVRPANEQH
MRKLNDLLRP FSYGSDDLYE ETQPFVKETS LANVDIQICQ TRPKVPPGKM WIFESRTIYD
ENGNCVSARE GAMEVSLASR MHNEPIVIVS MNDSMCF
