ID   ELMOA_DICDI             Reviewed;         977 AA.
AC   Q54YW1;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=ELMO domain-containing protein A;
GN   Name=elmoA; ORFNames=DDB_G0278051;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   FUNCTION, INTERACTION WITH MHCA, AND DISRUPTION PHENOTYPE.
RX   PubMed=18854143; DOI=10.1016/j.devcel.2008.08.006;
RA   Isik N., Brzostowski J.A., Jin T.;
RT   "An Elmo-like protein associated with myosin II restricts spurious F-actin
RT   events to coordinate phagocytosis and chemotaxis.";
RL   Dev. Cell 15:590-602(2008).
CC   -!- FUNCTION: Functions as a negative regulator of actin polymerization.
CC       Modulates actin/myosin II at cortex actinomyosins to prevent excessive
CC       F-actin polymerization around the cell periphery, thereby maintaining
CC       proper cell shape during phagocytosis and chemotaxis.
CC       {ECO:0000269|PubMed:18854143}.
CC   -!- SUBUNIT: Associates with mhcA.
CC   -!- INTERACTION:
CC       Q54YW1; P08799: mhcA; NbExp=2; IntAct=EBI-2928498, EBI-2928504;
CC   -!- DISRUPTION PHENOTYPE: Null mutants show the remarkable ability to
CC       engulf multiple particles simultaneously, also positively respond to a
CC       cAMP gradient but form spurious peudopods which are normally suppressed
CC       to promote efficient movement up a chemical gradient.
CC       {ECO:0000269|PubMed:18854143}.
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DR   EMBL; AAFI02000023; EAL68198.1; -; Genomic_DNA.
DR   RefSeq; XP_642091.1; XM_636999.1.
DR   AlphaFoldDB; Q54YW1; -.
DR   SMR; Q54YW1; -.
DR   IntAct; Q54YW1; 1.
DR   STRING; 44689.DDB0233912; -.
DR   PaxDb; Q54YW1; -.
DR   EnsemblProtists; EAL68198; EAL68198; DDB_G0278051.
DR   GeneID; 8621302; -.
DR   KEGG; ddi:DDB_G0278051; -.
DR   dictyBase; DDB_G0278051; elmoA.
DR   eggNOG; KOG2998; Eukaryota.
DR   HOGENOM; CLU_304284_0_0_1; -.
DR   InParanoid; Q54YW1; -.
DR   OMA; FEEVYCT; -.
DR   Reactome; R-DDI-8849471; PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.
DR   PRO; PR:Q54YW1; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005737; C:cytoplasm; IDA:dictyBase.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0045159; F:myosin II binding; IPI:dictyBase.
DR   GO; GO:0007015; P:actin filament organization; IBA:GO_Central.
DR   GO; GO:0048870; P:cell motility; IMP:dictyBase.
DR   GO; GO:0030837; P:negative regulation of actin filament polymerization; IMP:dictyBase.
DR   GO; GO:0050764; P:regulation of phagocytosis; IMP:dictyBase.
DR   GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR006816; ELMO_dom.
DR   Pfam; PF04727; ELMO_CED12; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS51335; ELMO; 1.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..977
FT                   /note="ELMO domain-containing protein A"
FT                   /id="PRO_0000333278"
FT   DOMAIN          383..561
FT                   /note="ELMO"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00664"
FT   REGION          792..899
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   977 AA;  109938 MW;  7B0627F1043890A9 CRC64;
     MKIKVTFQER IIEHNFETEI TNLQQVTQKL CSLFLITNYY SYSLFLSSGQ LVDNINLIDE
     GSEIIIKHLN RLGTISIGSG YSNSVVGTTN NNAPSSPSSS ININGQQIQQ QIQQQIQQQQ
     QQQQQQQQQV QLSPDTLINN LLNNLKDNTF KKKAFFDLKD LKEEILIKKF VEKNGIEVIV
     CQLKELTGNT LSYALSALQT IMSYEFTITS MTSTDTASLI TQLLPLTENT SNPSISKTSL
     SLLCLFLNQS NNLNFKQFSS TLVLEYNEKT KRNYNHTLVQ LLSSSNTVDV QLNALTLINI
     IIGKTMSTTI PGLENGSVTG GENGFNKLLK ELDEYEINQK LKKLVESIIV APELKRQLYI
     YQRHRFQVIT NRKNVTFNKE SSEHDALLMK LWSLTYPGVK LESRVSEQWK QMGFQGTDPC
     TDFRAMGIWG LDNLIYFAQN YNEKFRKIVN SQIDRKEREY PTATAGIVLT FELYNSIFKM
     GTPNLNPYNS TTSNTTSNTT STTNIDDLPF FPLFFSHPHA FEEVYCTTFQ ILDSTWDDMN
     GTYMHFQKIM SSVKNLIITA LESKPTTLEA FDWKCQKNTK NSNGGTNSNQ NNSSSNLLLS
     NFANGSSLLS LLNDLSSSSR DDMKKLLTGV NYQVLDLIKS QKISYFQEGF QFKLHKQLKT
     KQSLPLNWIF IRLFNNNNNN NNNNENCSYE IQYCFLSTEL NQPPLPNQSI PTNYNTIKIS
     DLFFNGESTN SNNKKKDKSL SYFNISIKDE QIQSLIQNQL PLSNLNNSSN SLQLDSSTSM
     NSIKDSIINI SSSNNNIKDN LTNNNTNTNT NNTNNNTSNG NGNSNSVSMS SININNSGQL
     SPNTTSPILI PQQQQQQPQS SSLSVQHQQS STPTPSSPVL LSSPSLQSSS SSSSSSSNPN
     LFTIDLISSN RDDVSNFWDS IKLLSGQEIK SQEGLDDYHS LLSINTSVKL LDLDGIDIPK
     ETPQIPILPD NFDFRTV