ID   AGALB_NEOFI             Reviewed;         447 AA.
AC   A1D0A3;
DT   23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Probable alpha-galactosidase B;
DE            EC=3.2.1.22;
DE   AltName: Full=Melibiase B;
DE   Flags: Precursor;
GN   Name=aglB; ORFNames=NFIA_039990;
OS   Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164
OS   / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=331117;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181
RC   / WB 181;
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA   Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA   Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA   Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA   Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA   White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA   Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
CC   -!- FUNCTION: Hydrolyzes a variety of simple alpha-D-galactoside as well as
CC       more complex molecules such as oligosaccharides and polysaccharides.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC         residues in alpha-D-galactosides, including galactose
CC         oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 27 family. {ECO:0000305}.
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DR   EMBL; DS027686; EAW24423.1; -; Genomic_DNA.
DR   RefSeq; XP_001266320.1; XM_001266319.1.
DR   AlphaFoldDB; A1D0A3; -.
DR   SMR; A1D0A3; -.
DR   STRING; 36630.CADNFIAP00003149; -.
DR   EnsemblFungi; EAW24423; EAW24423; NFIA_039990.
DR   GeneID; 4593097; -.
DR   KEGG; nfi:NFIA_039990; -.
DR   VEuPathDB; FungiDB:NFIA_039990; -.
DR   eggNOG; KOG2366; Eukaryota.
DR   HOGENOM; CLU_013093_2_2_1; -.
DR   OMA; NAFGCDI; -.
DR   OrthoDB; 964130at2759; -.
DR   Proteomes; UP000006702; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0052692; F:raffinose alpha-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd14792; GH27; 1.
DR   Gene3D; 2.60.40.1180; -; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR002241; Glyco_hydro_27.
DR   InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR041233; Melibiase_C.
DR   PANTHER; PTHR11452; PTHR11452; 1.
DR   Pfam; PF16499; Melibiase_2; 2.
DR   Pfam; PF17801; Melibiase_C; 1.
DR   PRINTS; PR00740; GLHYDRLASE27.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Glycoprotein; Glycosidase; Hydrolase; Reference proteome;
KW   Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..447
FT                   /note="Probable alpha-galactosidase B"
FT                   /id="PRO_0000393221"
FT   ACT_SITE        155
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        247
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         225..229
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        162
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        180
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        236
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        286
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        45..77
FT                   /evidence="ECO:0000250"
FT   DISULFID        127..157
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   447 AA;  49164 MW;  23D44A56458820FB CRC64;
     MTTFLSLTTA AAVLTLARGS NALVRPGNVG KLPALGWNSW NAFGCDIDAA KIMTAANEVV
     NLGLKDLGYE YINIDDCWSV KSGRDASTQR MVPDPEKFPD GISGLADQIH DLGLKVGIYS
     SAGLTTCAGY PASLGYEDID AQTFAEWGID YLKYDNCGVP SNWTDAYTYC VPDPGSKSTN
     GTCPDNKNPA PAGYDWRTSL TAERYRRMRD ALVSVDRTIL YSLCNWGQAD VNDWGNETGN
     SWRTTGDITP SWPRIAAIAN ENSFLMNYVD FWGYPDPDML EVGNGNLTLA ENRAHFALWA
     AMKSPLIIGT ALDSISQDHL AILSNKILLK FHQDPVVGRP AHPYKWGYNP DWTFDPAHPA
     EYWSGASSVL GGTLVLMLNS EDTKQRRTAV WKEIPELKDV LGRQGKRRTG FRVTDVWTGK
     DLGCVRDHYS VELESHDVAA LVVGRAC