ELOA1_CAEEL
ID ELOA1_CAEEL Reviewed; 434 AA.
AC Q09413;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Transcription elongation factor B polypeptide 3;
DE AltName: Full=Elongin-A;
DE AltName: Full=RNA polymerase II transcription factor SIII subunit A;
GN Name=tceb-3 {ECO:0000312|WormBase:R03D7.4};
GN ORFNames=R03D7.4 {ECO:0000312|WormBase:R03D7.4};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, SUBUNIT, AND MUTAGENESIS OF 1-MET--LYS-201 AND 1-MET--LYS-93.
RX PubMed=8896449; DOI=10.1002/j.1460-2075.1996.tb00940.x;
RA Aso T., Haque D., Barstead R.J., Conaway R.C., Conaway J.W.;
RT "The inducible elongin A elongation activation domain: structure, function
RT and interaction with the elongin BC complex.";
RL EMBO J. 15:5557-5566(1996).
CC -!- FUNCTION: SIII, also known as elongin, is a general transcription
CC elongation factor that increases the RNA polymerase II transcription
CC elongation past template-encoded arresting sites. Subunit A is
CC transcriptionally active and its transcription activity is strongly
CC enhanced by binding to the dimeric complex of the SIII regulatory
CC subunits B and C (elongin BC complex). {ECO:0000269|PubMed:8896449}.
CC -!- SUBUNIT: Heterotrimer of an A, B and C subunit.
CC {ECO:0000305|PubMed:8896449}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q63187}.
CC -!- DOMAIN: The BC-box, which mediates binding to the elongin BC complex,
CC has the consensus [APST]-L-x(3)-C-x(3)-[AILV].
CC {ECO:0000250|UniProtKB:Q63187}.
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DR EMBL; BX284602; CAA86857.1; -; Genomic_DNA.
DR PIR; D88305; D88305.
DR PIR; S72430; S72430.
DR RefSeq; NP_496355.1; NM_063954.5.
DR AlphaFoldDB; Q09413; -.
DR SMR; Q09413; -.
DR BioGRID; 39993; 1.
DR IntAct; Q09413; 2.
DR STRING; 6239.R03D7.4; -.
DR EPD; Q09413; -.
DR PaxDb; Q09413; -.
DR PeptideAtlas; Q09413; -.
DR EnsemblMetazoa; R03D7.4.1; R03D7.4.1; WBGene00010990.
DR EnsemblMetazoa; R03D7.4.2; R03D7.4.2; WBGene00010990.
DR GeneID; 174683; -.
DR KEGG; cel:CELE_R03D7.4; -.
DR UCSC; R03D7.4; c. elegans.
DR CTD; 174683; -.
DR WormBase; R03D7.4; CE01611; WBGene00010990; tceb-3.
DR eggNOG; KOG2821; Eukaryota.
DR GeneTree; ENSGT00390000002428; -.
DR HOGENOM; CLU_636539_0_0_1; -.
DR InParanoid; Q09413; -.
DR OMA; VIDMAHT; -.
DR OrthoDB; 1063933at2759; -.
DR PhylomeDB; Q09413; -.
DR Reactome; R-CEL-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-CEL-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-CEL-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-CEL-75955; RNA Polymerase II Transcription Elongation.
DR PRO; PR:Q09413; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00010990; Expressed in embryo and 4 other tissues.
DR GO; GO:0070449; C:elongin complex; IEA:InterPro.
DR GO; GO:0008023; C:transcription elongation factor complex; IDA:UniProtKB.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IDA:UniProtKB.
DR InterPro; IPR010684; RNA_pol_II_trans_fac_SIII_A.
DR Pfam; PF06881; Elongin_A; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..434
FT /note="Transcription elongation factor B polypeptide 3"
FT /id="PRO_0000086958"
FT DOMAIN 237..281
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT REGION 142..161
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 221..230
FT /note="BC box"
FT /evidence="ECO:0000250|UniProtKB:Q63187"
FT REGION 391..415
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 394..415
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 1..201
FT /note="Missing: Reduces ability to stimulate elongation by
FT rat RNA polymerase II."
FT /evidence="ECO:0000269|PubMed:8896449"
FT MUTAGEN 1..93
FT /note="Missing: Stimulates elongation by rat RNA polymerase
FT II with the same activity as wild-type."
FT /evidence="ECO:0000269|PubMed:8896449"
SQ SEQUENCE 434 AA; 49240 MW; EB0A02A996ED0844 CRC64;
MPETDEEKVR RYTECLMNGI DPKRALKRLY DLNVSPEVFK SADTYQCVKR YESSPELAKY
AKRVRDKLLG GRKREKGGGE DDADIEHTAL KKAKKEEVNL DEEFAEAMKS GVSAQASSAP
RATVDYSKYK VVKRVEVKVE PKPEPVDVHE QQASSSSMSY QREHQKDYAP VVPTCKPSGQ
PKKAIPQSKS LHADENMFKP RKERQKVFAG RRKRVGEGVS TLVSLCQTVL MSHIDMIDHV
GIVPFDLLKP VLDHASTDQL RHILDVNPML VEDADEMFHE MVSREFPKYA NREKSGWTWR
EMYDRLVEKK QKKENDKLEM LTSRIGKSNS AQSQGRQTMV IDMAHTRVRS KSFFNTVKDS
QVKMSATPSA LQLSQARKNV KIEGKAQLRT ITPRGGGVPS TSRSRSNNNN NMNNGLVVKK
TAPLMAKCKK MLKR
