ELOA2_HUMAN
ID ELOA2_HUMAN Reviewed; 753 AA.
AC Q8IYF1; Q9P2V9;
DT 11-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 17-APR-2007, sequence version 2.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Elongin-A2;
DE Short=EloA2;
DE AltName: Full=RNA polymerase II transcription factor SIII subunit A2;
DE AltName: Full=Transcription elongation factor B polypeptide 3B;
GN Name=ELOA2 {ECO:0000312|HGNC:HGNC:30771}; Synonyms=TCEB3B, TCEB3L;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, TISSUE SPECIFICITY, AND
RP VARIANTS PRO-179; PHE-254; SER-403 AND THR-446.
RC TISSUE=Testis;
RX PubMed=10692460; DOI=10.1074/jbc.275.9.6546;
RA Aso T., Yamazaki K., Amimoto K., Kuroiwa A., Higashi H., Matsuda Y.,
RA Kitajima S., Hatakeyama M.;
RT "Identification and characterization of Elongin A2, a new member of the
RT Elongin family of transcription elongation factors, specifically expressed
RT in the testis.";
RL J. Biol. Chem. 275:6546-6552(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP VARIANT [LARGE SCALE ANALYSIS] GLN-498.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- FUNCTION: SIII, also known as elongin, is a general transcription
CC elongation factor that increases the RNA polymerase II transcription
CC elongation past template-encoded arresting sites. Subunit A2 is
CC transcriptionally active but its transcription activity is not enhanced
CC by binding to the dimeric complex of the SIII regulatory subunits B and
CC C (elongin BC complex). {ECO:0000269|PubMed:10692460}.
CC -!- SUBUNIT: Heterotrimer of an A (ELOA, ELOA2 or ELOA3P), ELOB and ELOC
CC subunit. {ECO:0000269|PubMed:10692460}.
CC -!- INTERACTION:
CC Q8IYF1; O14647-3: CHD2; NbExp=3; IntAct=EBI-741705, EBI-11985957;
CC Q8IYF1; O75031: HSF2BP; NbExp=5; IntAct=EBI-741705, EBI-7116203;
CC Q8IYF1; Q6ZUT1: NKAPD1; NbExp=3; IntAct=EBI-741705, EBI-3920396;
CC Q8IYF1; Q15287: RNPS1; NbExp=3; IntAct=EBI-741705, EBI-395959;
CC Q8IYF1; Q8N9Q2: SREK1IP1; NbExp=3; IntAct=EBI-741705, EBI-10268630;
CC Q8IYF1; P40337-2: VHL; NbExp=3; IntAct=EBI-741705, EBI-12157263;
CC Q8IYF1; Q8TBK6: ZCCHC10; NbExp=3; IntAct=EBI-741705, EBI-597063;
CC Q8IYF1; P52744: ZNF138; NbExp=3; IntAct=EBI-741705, EBI-10746567;
CC Q8IYF1; P17027: ZNF23; NbExp=3; IntAct=EBI-741705, EBI-5657766;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in testis.
CC {ECO:0000269|PubMed:10692460}.
CC -!- DOMAIN: The BC-box, which mediates binding to the elongin BC complex,
CC has the consensus sequence [APST]-L-x(3)-C-x(3)-[AILV].
CC {ECO:0000250|UniProtKB:Q63187}.
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DR EMBL; AB030834; BAA90665.1; -; mRNA.
DR EMBL; BC036022; AAH36022.1; -; mRNA.
DR CCDS; CCDS11932.1; -.
DR RefSeq; NP_057511.2; NM_016427.2.
DR AlphaFoldDB; Q8IYF1; -.
DR SMR; Q8IYF1; -.
DR BioGRID; 119388; 66.
DR IntAct; Q8IYF1; 23.
DR STRING; 9606.ENSP00000331302; -.
DR iPTMnet; Q8IYF1; -.
DR PhosphoSitePlus; Q8IYF1; -.
DR BioMuta; ELOA2; -.
DR DMDM; 145559470; -.
DR MassIVE; Q8IYF1; -.
DR PaxDb; Q8IYF1; -.
DR PeptideAtlas; Q8IYF1; -.
DR PRIDE; Q8IYF1; -.
DR Antibodypedia; 22536; 76 antibodies from 18 providers.
DR DNASU; 51224; -.
DR Ensembl; ENST00000332567.6; ENSP00000331302.4; ENSG00000206181.7.
DR GeneID; 51224; -.
DR KEGG; hsa:51224; -.
DR MANE-Select; ENST00000332567.6; ENSP00000331302.4; NM_016427.3; NP_057511.2.
DR UCSC; uc002lcr.2; human.
DR CTD; 51224; -.
DR DisGeNET; 51224; -.
DR GeneCards; ELOA2; -.
DR HGNC; HGNC:30771; ELOA2.
DR HPA; ENSG00000206181; Group enriched (brain, choroid plexus, testis).
DR MIM; 609522; gene.
DR neXtProt; NX_Q8IYF1; -.
DR OpenTargets; ENSG00000206181; -.
DR PharmGKB; PA134932220; -.
DR VEuPathDB; HostDB:ENSG00000206181; -.
DR eggNOG; KOG2821; Eukaryota.
DR GeneTree; ENSGT00390000002428; -.
DR HOGENOM; CLU_021679_1_0_1; -.
DR InParanoid; Q8IYF1; -.
DR OMA; CNSGASF; -.
DR OrthoDB; 1174517at2759; -.
DR PhylomeDB; Q8IYF1; -.
DR TreeFam; TF317259; -.
DR PathwayCommons; Q8IYF1; -.
DR Reactome; R-HSA-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-HSA-167152; Formation of HIV elongation complex in the absence of HIV Tat.
DR Reactome; R-HSA-167200; Formation of HIV-1 elongation complex containing HIV-1 Tat.
DR Reactome; R-HSA-167238; Pausing and recovery of Tat-mediated HIV elongation.
DR Reactome; R-HSA-167243; Tat-mediated HIV elongation arrest and recovery.
DR Reactome; R-HSA-167246; Tat-mediated elongation of the HIV-1 transcript.
DR Reactome; R-HSA-167287; HIV elongation arrest and recovery.
DR Reactome; R-HSA-167290; Pausing and recovery of HIV elongation.
DR Reactome; R-HSA-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-HSA-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-HSA-75955; RNA Polymerase II Transcription Elongation.
DR SignaLink; Q8IYF1; -.
DR BioGRID-ORCS; 51224; 10 hits in 1062 CRISPR screens.
DR GenomeRNAi; 51224; -.
DR Pharos; Q8IYF1; Tdark.
DR PRO; PR:Q8IYF1; -.
DR Proteomes; UP000005640; Chromosome 18.
DR RNAct; Q8IYF1; protein.
DR Bgee; ENSG00000206181; Expressed in sperm and 4 other tissues.
DR Genevisible; Q8IYF1; HS.
DR GO; GO:0070449; C:elongin complex; IEA:InterPro.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; TAS:ProtInc.
DR GO; GO:0006366; P:transcription by RNA polymerase II; TAS:ProtInc.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IEA:InterPro.
DR Gene3D; 1.20.930.10; -; 1.
DR InterPro; IPR010684; RNA_pol_II_trans_fac_SIII_A.
DR InterPro; IPR003617; TFIIS/CRSP70_N_sub.
DR InterPro; IPR035441; TFIIS/LEDGF_dom_sf.
DR InterPro; IPR017923; TFIIS_N.
DR Pfam; PF06881; Elongin_A; 1.
DR Pfam; PF08711; Med26; 1.
DR SMART; SM00509; TFS2N; 1.
DR SUPFAM; SSF47676; SSF47676; 1.
DR PROSITE; PS51319; TFIIS_N; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..753
FT /note="Elongin-A2"
FT /id="PRO_0000086963"
FT DOMAIN 5..80
FT /note="TFIIS N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00649"
FT REGION 80..245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 261..453
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 477..497
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 500..659
FT /note="Activation domain"
FT /evidence="ECO:0000250"
FT REGION 528..537
FT /note="BC-box"
FT /evidence="ECO:0000250|UniProtKB:Q63187"
FT REGION 528..537
FT /note="Interacting with Elongin BC complex"
FT /evidence="ECO:0000250"
FT REGION 650..735
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 143..162
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 299..323
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 336..352
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 411..425
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 477..491
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 652..671
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 679..698
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 179
FT /note="R -> P (in dbSNP:rs2571028)"
FT /evidence="ECO:0000269|PubMed:10692460"
FT /id="VAR_050965"
FT VARIANT 254
FT /note="C -> F (in dbSNP:rs2010834)"
FT /evidence="ECO:0000269|PubMed:10692460"
FT /id="VAR_050966"
FT VARIANT 403
FT /note="A -> S (in dbSNP:rs892586)"
FT /evidence="ECO:0000269|PubMed:10692460"
FT /id="VAR_050967"
FT VARIANT 446
FT /note="A -> T (in dbSNP:rs3744863)"
FT /evidence="ECO:0000269|PubMed:10692460"
FT /id="VAR_061646"
FT VARIANT 498
FT /note="R -> Q (in a colorectal cancer sample; somatic
FT mutation; dbSNP:rs138369139)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_035914"
FT CONFLICT 55
FT /note="R -> L (in Ref. 1; BAA90665)"
FT /evidence="ECO:0000305"
FT CONFLICT 236
FT /note="P -> L (in Ref. 1; BAA90665)"
FT /evidence="ECO:0000305"
FT CONFLICT 456
FT /note="S -> N (in Ref. 2; AAH36022)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 753 AA; 83921 MW; A4CFDF5A0D3AD529 CRC64;
MAAGSTTLHA VEKLQVRLAT KTEPKKLEKY LQKLSALPMT ADILAETGIR KTVKRLRKHQ
HVGDFARDLA ARWKKLVLVD RNTRPGPQDP EESASRQRFG EALQDQEKAW GFPENATAPR
SPSHSPEHRR TARRTPPGQQ RPHPRSHSRE PRAERKCPRI APADSGRYRA SPTRTAPLRM
PEGPEPAAPG KQPGRGHTHA AQGGPLLCPG CQGQPQGKAV VSHSKGHKSS RQEKRPLCAQ
GDWHSPTLIR EKSCGACLRE ETPRMPSWAS ARDRQPSDFK TDKEGGQAGS GQRVPALEEA
PDSHQKRPQH SHSNKKRPSL DGRDPGNGTH GLSPEEKEQL SNDRETQEGK PPTAHLDRTS
VSSLSEVEEV DMAEEFEQPT LSCEKYLTYD QLRKQKKKTG KSATTALGDK QRKANESKGT
RESWDSAKKL PPVQESQSER LQAAGADSAG PKTVPSHVFS ELWDLSEAWM QANYDPLSDS
DSMTSQAKPE ALSSPKFREE AAFPGRRVNA KMPVYSGSRP ACQLQVPTLR QQCAQVLRNN
PDALSDVGEV PYWVLEPVLE GWRPDQLYRR KKDNHALVRE TDELRRNHCF QDFKEEKPQE
NKTWREQYLR LPDAPEQRLR VMTTNIRSAR GNNPNGREAK MICFKSVAKT PYDTSRRQEK
SAGDADPENG EIKPASKPAG SSHTPSSQSS SGGGRDSSSS ILRWLPEKRA NPCLSSSNEH
AAPAAKTRKQ AAKKVAPLMA KAIRDYKRRF SRR
