ID   AGALD_ASPFC             Reviewed;         648 AA.
AC   B0YEK2;
DT   15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT   15-JUN-2010, sequence version 2.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=Probable alpha-galactosidase D;
DE            EC=3.2.1.22;
DE   AltName: Full=Melibiase D;
DE   Flags: Precursor;
GN   Name=aglD; ORFNames=AFUB_099470;
OS   Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus
OS   fumigatus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=451804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CEA10 / CBS 144.89 / FGSC A1163;
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA   Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA   Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA   Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA   Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA   White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA   Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
CC   -!- FUNCTION: Hydrolyzes a variety of simple alpha-D-galactoside as well as
CC       more complex molecules such as oligosaccharides and polysaccharides.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC         residues in alpha-D-galactosides, including galactose
CC         oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 27 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EDP47346.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; DS499603; EDP47346.1; ALT_SEQ; Genomic_DNA.
DR   AlphaFoldDB; B0YEK2; -.
DR   SMR; B0YEK2; -.
DR   PhylomeDB; B0YEK2; -.
DR   Proteomes; UP000001699; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0052692; F:raffinose alpha-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd14792; GH27; 1.
DR   Gene3D; 2.60.40.1180; -; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR002241; Glyco_hydro_27.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR041233; Melibiase_C.
DR   PANTHER; PTHR11452; PTHR11452; 1.
DR   Pfam; PF16499; Melibiase_2; 1.
DR   Pfam; PF17801; Melibiase_C; 1.
DR   PRINTS; PR00740; GLHYDRLASE27.
DR   SUPFAM; SSF51445; SSF51445; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Disulfide bond; Glycoprotein; Glycosidase;
KW   Hydrolase; Polysaccharide degradation; Secreted; Signal.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255"
FT   CHAIN           17..648
FT                   /note="Probable alpha-galactosidase D"
FT                   /id="PRO_0000395070"
FT   ACT_SITE        154
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        221
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         199..203
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        84
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        90
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        339
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        350
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        505
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        572
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        123..156
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   648 AA;  70100 MW;  DA34A68726860416 CRC64;
     MEFIVSLLLL SPALVAGSLH SRIDNGLART PQMGWNSYNY YSCSPNEAII RSNAKALVDL
     GLAELGYRYV TTDCGWSVAD RLPNGTLTWN ETLFPSGFPA MGEYLHELGL LFGVYGDSGT
     KLCGSPPDQV GSLYHEEQDA KTFAEWGADS LKYDNCYSDA ATNYPNVNYE PSTSPRPRYE
     IMSSALARVG RPILFQICEW GIDFPALWAP ALGNSWRIGN DIIPAWRSIF RTLNQAVPNT
     DFAGPGQWAD LDMLYVGNGV FSLPEEQTHF SLWAILKSPL TIGAALKDDD TSISQASLEV
     LKQKDVIGFN QDALGVSASL KRRWSDEGYE VWSGPLSGNR TVVAVINWRN ESRDLTLDLP
     DVGLQYAQVA RNIWGKTVVR DVRTSYTAGV AGHGTILLEL QGTLPSGLYP AKIFAKSTGQ
     RSTFESIYAA TTSANYELAI TFSRPSTETV TITTSSGQTV SISGKSGRIA LTAGSNTITI
     QHKTPIESIQ ITPPTGTYYA NTVFNVTGSA KHTTCGSGCS PVGSKIGYLS PTSNAYTSIS
     TTTAGSKYLA IDYINNEVAF SSSWGWGSNS RNLTVSVNDG APVRLEVPLS GRHSELYSPG
     KGWWDTATLG VLTSGWKKGE NKVVFGNEGG EDGFQTYAAD FVGVRVLD