ID   ELP2_DANRE              Reviewed;         821 AA.
AC   Q05AM5; Q1LUN8;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Elongator complex protein 2;
DE            Short=ELP2;
GN   Name=elp2; ORFNames=si:dkey-190l1.1, zgc:153559;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the elongator complex which is required for
CC       multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl
CC       uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U
CC       (5-carbamoylmethyl uridine). The elongator complex catalyzes the
CC       formation of carboxymethyluridine in the wobble base at position 34 in
CC       tRNAs. {ECO:0000250|UniProtKB:Q6IA86}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000250|UniProtKB:Q6IA86}.
CC   -!- SUBUNIT: Component of the elongator complex.
CC       {ECO:0000250|UniProtKB:Q6IA86}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q6IA86}. Nucleus
CC       {ECO:0000250|UniProtKB:Q6IA86}.
CC   -!- DOMAIN: Folds into a two seven-bladed beta-propeller structure which is
CC       required for elongator complex assembly.
CC       {ECO:0000250|UniProtKB:P42935}.
CC   -!- SIMILARITY: Belongs to the WD repeat ELP2 family. {ECO:0000305}.
CC   -!- CAUTION: The elongator complex was originally thought to play a role in
CC       transcription elongation. However, it is no longer thought to play a
CC       direct role in this process and its primary function is thought to be
CC       in tRNA modification. {ECO:0000250|UniProtKB:Q6IA86}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAK05339.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; BX927389; CAK05339.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; BC124388; AAI24389.1; -; mRNA.
DR   AlphaFoldDB; Q05AM5; -.
DR   SMR; Q05AM5; -.
DR   STRING; 7955.ENSDARP00000104754; -.
DR   PaxDb; Q05AM5; -.
DR   PeptideAtlas; Q05AM5; -.
DR   ZFIN; ZDB-GENE-060503-525; elp2.
DR   eggNOG; KOG1063; Eukaryota.
DR   InParanoid; Q05AM5; -.
DR   PhylomeDB; Q05AM5; -.
DR   TreeFam; TF105985; -.
DR   UniPathway; UPA00988; -.
DR   PRO; PR:Q05AM5; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0033588; C:elongator holoenzyme complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR   Gene3D; 2.130.10.10; -; 6.
DR   InterPro; IPR037289; Elp2.
DR   InterPro; IPR011048; Haem_d1_sf.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR44111; PTHR44111; 1.
DR   Pfam; PF00400; WD40; 5.
DR   SMART; SM00320; WD40; 11.
DR   SUPFAM; SSF50978; SSF50978; 2.
DR   SUPFAM; SSF51004; SSF51004; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 6.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT   CHAIN           1..821
FT                   /note="Elongator complex protein 2"
FT                   /id="PRO_0000284000"
FT   REPEAT          14..53
FT                   /note="WD 1"
FT   REPEAT          54..98
FT                   /note="WD 2"
FT   REPEAT          103..145
FT                   /note="WD 3"
FT   REPEAT          151..193
FT                   /note="WD 4"
FT   REPEAT          198..239
FT                   /note="WD 5"
FT   REPEAT          271..319
FT                   /note="WD 6"
FT   REPEAT          329..368
FT                   /note="WD 7"
FT   REPEAT          374..413
FT                   /note="WD 8"
FT   REPEAT          424..462
FT                   /note="WD 9"
FT   REPEAT          602..641
FT                   /note="WD 10"
FT   REPEAT          656..695
FT                   /note="WD 11"
FT   REPEAT          715..754
FT                   /note="WD 12"
FT   REPEAT          769..821
FT                   /note="WD 13"
FT   CONFLICT        689
FT                   /note="V -> G (in Ref. 1; CAK05339)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   821 AA;  90083 MW;  D2DCBF260C16C2ED CRC64;
     MAAPVMDTCH VSCCANRAPN VVSWGRGGLI AFGTCNSVAI YNPEEIRVVD LLNKHTGRVN
     AVQWVHEPDC SPENQLISGG SDNNVIVWEK LDGKFRACAV CSGHSGPVCA VDAVSLSSSH
     LLVASASSDS TVKLWTYSSD AAECLQTVAF GSGFMMDVSL ALLPGSRVPV LACGGDDSRV
     HLYVQLSGQF QRVLTLTGHE DWVRGVEWAN KDGELWLASC SQDCLIRVWR LFAKTAAEPD
     LQTDGIIKMK ENIFQVSGEE FAVTLETVLA GHENWVYGIH WQPPSVKGDS VEQSLKLLSA
     SMDKTMILWG PEEDSGMWVE MVRVGEVGGN TLGFYGCQMS PDGSMILAHA FHGALHLWHH
     DSNQEWRPSV VISGHFNAVQ DMSWDPEGEF IITVGSDQTT RLFTPWTRKG SSQITWHEIS
     RPQIHGYDMQ CLTMVGRFQF VSGADEKVLR VFKAPRNFVE NFAHISGTSL EKLLGCNDIA
     DLPEGASTPA LGLSNKAVFQ GDLASPSPQQ DGGDFNSLSD QYKESYFQPL KLAEPPTEDD
     LLQNTLWPEV QKLYGHGFEM FCLASDCART VVASACKASK AEHASILLWS TASWKQLQSL
     SCHSLTITQM AFSPNGQLLL AVSRDRTWSL WRRGNPDLDT EAMFSLYANT SKDTSVHTRI
     IWSCDWSADN KYFVTSSRDK KVIIWGHAVS GVAVGEGEDA RVTSCSSVLD VGDSATAVSI
     CPFLCSDHSY LLAVGLENGQ ILLYKWKPLE DLSSESDWSR CKDTDACQGH TMVVKRLRWR
     PRLGRGGHGG QDSKEEQAWV QLASAGADHV VKIFDINLSA L