ID   ELP2_DICDI              Reviewed;         901 AA.
AC   Q86H45; Q552Y9;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=Elongator complex protein 2;
DE            Short=ELP2;
GN   Name=elp2; ORFNames=DDB_G0275651;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Component of the elongator complex which is required for
CC       multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl
CC       uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U
CC       (5-carbamoylmethyl uridine). The elongator complex catalyzes formation
CC       of carboxymethyluridine in the wobble base at position 34 in tRNAs.
CC       {ECO:0000250|UniProtKB:Q6IA86}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000250|UniProtKB:Q6IA86}.
CC   -!- SUBUNIT: Component of the elongator complex.
CC       {ECO:0000250|UniProtKB:Q6IA86}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q6IA86}. Nucleus
CC       {ECO:0000250|UniProtKB:Q6IA86}.
CC   -!- DOMAIN: Folds into a two seven-bladed beta-propeller structure which is
CC       required for elongator complex assembly.
CC       {ECO:0000250|UniProtKB:P42935}.
CC   -!- SIMILARITY: Belongs to the WD repeat ELP2 family. {ECO:0000305}.
CC   -!- CAUTION: The elongator complex was originally thought to play a role in
CC       transcription elongation. However, it is no longer thought to play a
CC       direct role in this process and its primary function is thought to be
CC       in tRNA modification. {ECO:0000250|UniProtKB:Q6IA86}.
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DR   EMBL; AAFI02000013; EAL69576.1; -; Genomic_DNA.
DR   RefSeq; XP_643531.1; XM_638439.1.
DR   AlphaFoldDB; Q86H45; -.
DR   SMR; Q86H45; -.
DR   STRING; 44689.DDB0231759; -.
DR   PaxDb; Q86H45; -.
DR   EnsemblProtists; EAL69576; EAL69576; DDB_G0275651.
DR   GeneID; 8620113; -.
DR   KEGG; ddi:DDB_G0275651; -.
DR   dictyBase; DDB_G0275651; elp2.
DR   eggNOG; KOG1063; Eukaryota.
DR   HOGENOM; CLU_006430_2_0_1; -.
DR   InParanoid; Q86H45; -.
DR   OMA; EHTKRVN; -.
DR   PhylomeDB; Q86H45; -.
DR   UniPathway; UPA00988; -.
DR   PRO; PR:Q86H45; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0033588; C:elongator holoenzyme complex; IEA:InterPro.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:dictyBase.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR   Gene3D; 2.130.10.10; -; 6.
DR   InterPro; IPR037289; Elp2.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR44111; PTHR44111; 1.
DR   Pfam; PF00400; WD40; 6.
DR   SMART; SM00320; WD40; 12.
DR   SUPFAM; SSF50978; SSF50978; 3.
DR   PROSITE; PS50082; WD_REPEATS_2; 4.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 3.
PE   3: Inferred from homology;
KW   Cytoplasm; Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT   CHAIN           1..901
FT                   /note="Elongator complex protein 2"
FT                   /id="PRO_0000327784"
FT   REPEAT          11..50
FT                   /note="WD 1"
FT   REPEAT          53..104
FT                   /note="WD 2"
FT   REPEAT          113..155
FT                   /note="WD 3"
FT   REPEAT          173..214
FT                   /note="WD 4"
FT   REPEAT          225..274
FT                   /note="WD 5"
FT   REPEAT          327..377
FT                   /note="WD 6"
FT   REPEAT          387..426
FT                   /note="WD 7"
FT   REPEAT          435..474
FT                   /note="WD 8"
FT   REPEAT          495..535
FT                   /note="WD 9"
FT   REPEAT          653..697
FT                   /note="WD 10"
FT   REPEAT          700..739
FT                   /note="WD 11"
FT   REPEAT          749..788
FT                   /note="WD 12"
FT   REPEAT          796..844
FT                   /note="WD 13"
FT   REPEAT          859..897
FT                   /note="WD 14"
FT   REGION          571..598
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        571..593
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   901 AA;  100804 MW;  7A9F21E538983317 CRC64;
     MNVSSDVDFI SVGCNCLGDC LVWGQNKLAA YGAQNFIALF DPIQSKVLAT LPGHKDRVNH
     ICWVPNINEE YKNRYSSYEN ELLSCSSDNT IISWKQIKGG LNYQYKVVEV LKGHSDSVTN
     ISVLDFPDGS LLMCSTSADN TVKLWRRESL TKENIDTDTL PKWECIQTID FTPKCMECSS
     LAFIPGTTIP LLAVGGLEPK IHIYIQNLDS TTATLQFKKL MSLQGHQDWI RSLSFKTINE
     GEGEGEEEEL ILASSSQDFK IRLWKISKFT AEKQKQQQLD ESGNGGANLL GSLSTQLSGV
     TSLSTKGYLF NCNSVKYIIL LDAVLSGHDD WVYSIHWSPA RRDQETGKKI QEQMLISASM
     DKTAIVWRPD RTTGIWIDES RVGDMGGNIL GQYGAVFSPC SQYILSHGYN GAFHFWKQNT
     NQKSSFWEPQ IVVSGHFGPV QDLMWSPDYS YFISCSTDRT LRLFSEWKRN NNNNNLENNK
     EQQIISWNEI ARPQIHGYDL ECFTFINKKT HVIVSGAEEK IMRAFVGSQN FVDTLLNISK
     VQPVNDGTQR PLAANQPSLG LSNKPYFTGG SDYNENIDNN NNNNNNNGNG EENTEDSIKM
     KMGGGDEGGG GEGMDTGYFE DEIPFNPEVL SEPPFEEHLL QSSLWPEIHK FYGHGNEIVA
     VACSADGMYL ASTCRASSAD QATVRIWNVS NWKECANLKG HTLTVVNLSF SHNSKYLLGV
     SRDRMWTLWE RSASNSEEPF VKVISAPKSH GRIIWSGSWS HDDKFFATGA RDKLVKVWNL
     DNIKDIKNAC ASTLPAFGSG VTCVEFAPKS SKFTGEHGDH LLAVGEDDGK ITIWKSTTST
     SNPKSLDWTC VHTISPLISH TLDVRRIRWR DTPTINGNSL TYQIVTCSVD HSVRIFNIKF
     D