ELP2_DROME
ID ELP2_DROME Reviewed; 794 AA.
AC Q7K4B3;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Elongator complex protein 2;
DE Short=ELP2;
DE AltName: Full=Stat3-interacting protein homolog;
GN Name=Elp2; Synonyms=StIP; ORFNames=CG11887;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION IN THE STAT PATHWAY.
RA Han L., Harrison D.;
RT "The function of StIP in the JAK/STAT pathway.";
RL (In) Abstracts of the 47th Annual Drosophila Research Conference, pp.25-25,
RL Houston (2006).
CC -!- FUNCTION: Component of the elongator complex which is required for
CC multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl
CC uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U
CC (5-carbamoylmethyl uridine) (By similarity). The elongator complex
CC catalyzes the formation of carboxymethyluridine in the wobble base at
CC position 34 in tRNAs (By similarity). Involved in the regulation of the
CC STAT pathway (Ref.4). {ECO:0000250|UniProtKB:Q6IA86,
CC ECO:0000269|Ref.4}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000250|UniProtKB:Q6IA86}.
CC -!- SUBUNIT: Component of the elongator complex.
CC {ECO:0000250|UniProtKB:Q6IA86}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q6IA86}. Nucleus
CC {ECO:0000250|UniProtKB:Q6IA86}.
CC -!- DOMAIN: Folds into a two seven-bladed beta-propeller structure which is
CC required for elongator complex assembly.
CC {ECO:0000250|UniProtKB:P42935}.
CC -!- SIMILARITY: Belongs to the WD repeat ELP2 family. {ECO:0000305}.
CC -!- CAUTION: The elongator complex was originally thought to play a role in
CC transcription elongation. However, it is no longer thought to play a
CC direct role in this process and its primary function is thought to be
CC in tRNA modification. {ECO:0000250|UniProtKB:Q6IA86}.
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DR EMBL; AE013599; AAF58765.2; -; Genomic_DNA.
DR EMBL; AY052019; AAK93443.1; -; mRNA.
DR RefSeq; NP_610600.1; NM_136756.4.
DR AlphaFoldDB; Q7K4B3; -.
DR SMR; Q7K4B3; -.
DR BioGRID; 61932; 2.
DR IntAct; Q7K4B3; 2.
DR STRING; 7227.FBpp0087307; -.
DR PaxDb; Q7K4B3; -.
DR PRIDE; Q7K4B3; -.
DR EnsemblMetazoa; FBtr0088212; FBpp0087307; FBgn0033540.
DR GeneID; 36123; -.
DR KEGG; dme:Dmel_CG11887; -.
DR CTD; 55250; -.
DR FlyBase; FBgn0033540; Elp2.
DR VEuPathDB; VectorBase:FBgn0033540; -.
DR eggNOG; KOG0645; Eukaryota.
DR eggNOG; KOG1063; Eukaryota.
DR GeneTree; ENSGT00390000000916; -.
DR HOGENOM; CLU_006430_1_0_1; -.
DR InParanoid; Q7K4B3; -.
DR OMA; EHTKRVN; -.
DR OrthoDB; 461632at2759; -.
DR PhylomeDB; Q7K4B3; -.
DR UniPathway; UPA00988; -.
DR BioGRID-ORCS; 36123; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36123; -.
DR PRO; PR:Q7K4B3; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033540; Expressed in wing disc and 35 other tissues.
DR Genevisible; Q7K4B3; DM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0033588; C:elongator holoenzyme complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR Gene3D; 2.130.10.10; -; 5.
DR InterPro; IPR037289; Elp2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR44111; PTHR44111; 1.
DR Pfam; PF00400; WD40; 7.
DR SMART; SM00320; WD40; 11.
DR SUPFAM; SSF50978; SSF50978; 3.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 7.
DR PROSITE; PS50294; WD_REPEATS_REGION; 3.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT CHAIN 1..794
FT /note="Elongator complex protein 2"
FT /id="PRO_0000284002"
FT REPEAT 55..93
FT /note="WD 1"
FT REPEAT 98..140
FT /note="WD 2"
FT REPEAT 147..188
FT /note="WD 3"
FT REPEAT 203..244
FT /note="WD 4"
FT REPEAT 286..328
FT /note="WD 5"
FT REPEAT 337..376
FT /note="WD 6"
FT REPEAT 384..423
FT /note="WD 7"
FT REPEAT 433..472
FT /note="WD 8"
FT REPEAT 557..601
FT /note="WD 9"
FT REPEAT 604..643
FT /note="WD 10"
FT REPEAT 654..693
FT /note="WD 11"
FT REPEAT 705..751
FT /note="WD 12"
FT REPEAT 759..794
FT /note="WD 13"
SQ SEQUENCE 794 AA; 88974 MW; F13A7F063716F9D0 CRC64;
MQVENLHTSV ACNRCTECAD WGPNGWIAYG ACNAIAIMDP KFQGNSAKVL FTLVEHTKRV
NTVRWLDCDK LLSGGDDAIA ILWELDETGT TKSFTLKGHT SGVNTVDGIR QQDGSWLLAT
AAADTTIKLW TFQDNNYVCF QTISLSDGFC FCLRLQLLPK SNQVLLAFSG DDETVSLWSE
QVETAGEGDS LGRQFQRKHK LTGHEDWVRG LDFVVDGEDL LLASGSQDNF IRLWRIAPRS
KEQMQENRVD LHQLSHNDDE IKVEEKILQL GKEAWYAVSL ESVLYGHEGW IYGVHWHKTP
DQELRLLSAS IDKTVIIWAP TEEGIWLEEV RVGEVGGNSV GFYGGKFSGD GHSIMAHSYQ
GGFHIWSQDP DRPQLWTPGV IVGGHYGEVR DLAWEHSGAY LMTASADQTT RLHAPWLQDG
ANPTWHELAR PQIHGYDMQA LALLSRYKFA SGAEEKIVRT FQAPANFIEN FRHISGIEND
DAGDVLLDSL PKGASVPSLG LSNKAVYKVD SEVESTSKTS KDEYPDNYFV PIALETPPQE
ETLMQNTLWP ELQKLYGHGY EIFALAATAD GSLLASTCKA SNAEHAQIIL WNPSNWKQIQ
KLSGHQLTVT QLSFSPDSRY LLSVSRDRRW CLYERQDSSV SYQLVASTDK SNGVHTRIIW
SCDWSHDGQF FVTSSRDGKV VVWKKEEDCK ESSLNGWQAN GVLELKNESI TAVAFSNSYL
SGTDDTYILA LGTETGLIKI YQFVRGAWKL LSDLNKSQAH HLTVRRLQFR PGKQLQLASC
GEDHLVRIYD IKLT
