ID   ELP2_XENTR              Reviewed;         824 AA.
AC   Q5EBD9;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Elongator complex protein 2;
DE            Short=ELP2;
GN   Name=elp2;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the elongator complex which is required for
CC       multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl
CC       uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U
CC       (5-carbamoylmethyl uridine). The elongator complex catalyzes the
CC       formation of carboxymethyluridine in the wobble base at position 34 in
CC       tRNAs. {ECO:0000250|UniProtKB:Q6IA86}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000250|UniProtKB:Q6IA86}.
CC   -!- SUBUNIT: Component of the elongator complex.
CC       {ECO:0000250|UniProtKB:Q6IA86}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q6IA86}. Nucleus
CC       {ECO:0000250|UniProtKB:Q6IA86}.
CC   -!- DOMAIN: Folds into a two seven-bladed beta-propeller structure which is
CC       required for elongator complex assembly.
CC       {ECO:0000250|UniProtKB:P42935}.
CC   -!- SIMILARITY: Belongs to the WD repeat ELP2 family. {ECO:0000305}.
CC   -!- CAUTION: The elongator complex was originally thought to play a role in
CC       transcription elongation. However, it is no longer thought to play a
CC       direct role in this process and its primary function is thought to be
CC       in tRNA modification. {ECO:0000250|UniProtKB:Q6IA86}.
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DR   EMBL; BC089753; AAH89753.1; -; mRNA.
DR   RefSeq; NP_001015799.1; NM_001015799.1.
DR   AlphaFoldDB; Q5EBD9; -.
DR   SMR; Q5EBD9; -.
DR   GeneID; 548516; -.
DR   KEGG; xtr:548516; -.
DR   CTD; 55250; -.
DR   Xenbase; XB-GENE-5752362; elp2.
DR   eggNOG; KOG1063; Eukaryota.
DR   HOGENOM; CLU_006430_1_0_1; -.
DR   InParanoid; Q5EBD9; -.
DR   OrthoDB; 461632at2759; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000008143; Chromosome 6.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0033588; C:elongator holoenzyme complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000502; C:proteasome complex; ISS:UniProtKB.
DR   GO; GO:0061133; F:endopeptidase activator activity; ISS:UniProtKB.
DR   GO; GO:0043248; P:proteasome assembly; ISS:UniProtKB.
DR   GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; ISS:UniProtKB.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR   Gene3D; 2.130.10.10; -; 6.
DR   InterPro; IPR037289; Elp2.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR44111; PTHR44111; 1.
DR   Pfam; PF00400; WD40; 6.
DR   SMART; SM00320; WD40; 12.
DR   SUPFAM; SSF50978; SSF50978; 3.
DR   PROSITE; PS50082; WD_REPEATS_2; 5.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT   CHAIN           1..824
FT                   /note="Elongator complex protein 2"
FT                   /id="PRO_0000284001"
FT   REPEAT          14..51
FT                   /note="WD 1"
FT   REPEAT          54..98
FT                   /note="WD 2"
FT   REPEAT          103..147
FT                   /note="WD 3"
FT   REPEAT          153..195
FT                   /note="WD 4"
FT   REPEAT          200..241
FT                   /note="WD 5"
FT   REPEAT          278..326
FT                   /note="WD 6"
FT   REPEAT          336..375
FT                   /note="WD 7"
FT   REPEAT          383..422
FT                   /note="WD 8"
FT   REPEAT          433..471
FT                   /note="WD 9"
FT   REPEAT          562..606
FT                   /note="WD 10"
FT   REPEAT          609..648
FT                   /note="WD 11"
FT   REPEAT          664..703
FT                   /note="WD 12"
FT   REPEAT          718..764
FT                   /note="WD 13"
FT   REPEAT          784..824
FT                   /note="WD 14"
SQ   SEQUENCE   824 AA;  92123 MW;  9BAF9A4F9B8666FA CRC64;
     MSAPIVQLAH AAFSVNRCPS CVSWSRTGIL ACATCHSIAL YDPQKNVVIS TLNGHTGRVN
     CVHWVQKNDC SAETELVSGG SDKKVIIWAI EKNKCEQPVA AEGHTEVVNA VHAVYTQRSE
     NELLIVSAAS DCTVRLWLRR NVKTECLQTL SFGKGFVLDV CISFLPGSLV PVLACGADDS
     RIHLYVLQNE QFEKTQVLHG HEDWIRGVEW AVTGQNLFLA SCAQDCLIRI WKIFRKTAKE
     NSKTEDENSI KLKENIFKVK EKDTETSYAV TLETVLTGHE NSIYAVHWQP SFSRDGSIVQ
     PMSLLSASMD KTMILWEPDE ESGVWLEQVR VGEVGGNTLG FLGCQFSPDK SMILAHAFHG
     ALHLWSRAPN KQNEWIPMVI ISGHFDSVQD MRWDPDGQFV ITVSADQTTR LFGPWKKKGQ
     SHVTWHEIAR PQIHGYDMQC LAMIGRFQFV SGADEKVLRV FAAPRNFIEN FSNISCISVE
     KLLLNEDTNL PEGATVPALG LSNKAVFHGE ILIQSTQEEG KYNSVSEQYS QPNFQPLNLT
     EPPTEDHLLQ NTLWPEVQKL YGHGYEIFAV ACNSAKTVIA SACKASKREH AAIILWSTTS
     WKQLQSLSYH NLTVTQMAFS PDDEFLLVVS RDRNWSLWKK QEGIPEQTEP TFTLYASTDK
     NSCVHSRIIW ACDWTPDSKY FVTGSRDKKV IVWGDCSLPS MNEEKSSALI RPCSSLLDTG
     DSITAVSVSQ VLALDGSYII AVGLDCGIIQ LYKWKHSGTV NDWLKCLAMD QSLLSHTLTV
     KQLCWRSCLG RAGHDDRDNG DWIQLASCGA DHCVKIFDVY RKAL