ID   ELP4_BOVIN              Reviewed;         423 AA.
AC   Q2TBH6;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Elongator complex protein 4;
GN   Name=ELP4;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the elongator complex which is required for
CC       multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl
CC       uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U
CC       (5-carbamoylmethyl uridine). The elongator complex catalyzes the
CC       formation of carboxymethyluridine in the wobble base at position 34 in
CC       tRNAs. {ECO:0000250|UniProtKB:Q96EB1}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000250|UniProtKB:Q96EB1}.
CC   -!- SUBUNIT: Component of the elongator complex which consists of ELP1,
CC       ELP2, ELP3, ELP4, ELP5 and ELP6. {ECO:0000250|UniProtKB:Q96EB1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q96EB1}. Nucleus
CC       {ECO:0000250|UniProtKB:Q96EB1}.
CC   -!- SIMILARITY: Belongs to the ELP4 family. {ECO:0000305}.
CC   -!- CAUTION: The elongator complex was originally thought to play a role in
CC       transcription elongation. However, it is no longer thought to play a
CC       direct role in this process and its primary function is thought to be
CC       in tRNA modification. {ECO:0000250|UniProtKB:Q96EB1}.
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DR   EMBL; BC110206; AAI10207.1; -; mRNA.
DR   RefSeq; NP_001033773.1; NM_001038684.2.
DR   AlphaFoldDB; Q2TBH6; -.
DR   SMR; Q2TBH6; -.
DR   STRING; 9913.ENSBTAP00000005990; -.
DR   PaxDb; Q2TBH6; -.
DR   Ensembl; ENSBTAT00000005990; ENSBTAP00000005990; ENSBTAG00000023831.
DR   GeneID; 614139; -.
DR   KEGG; bta:614139; -.
DR   CTD; 26610; -.
DR   VEuPathDB; HostDB:ENSBTAG00000023831; -.
DR   VGNC; VGNC:28457; ELP4.
DR   eggNOG; KOG3949; Eukaryota.
DR   GeneTree; ENSGT00390000001443; -.
DR   InParanoid; Q2TBH6; -.
DR   OMA; NTTMWDD; -.
DR   OrthoDB; 973442at2759; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000009136; Chromosome 15.
DR   Bgee; ENSBTAG00000023831; Expressed in oocyte and 103 other tissues.
DR   ExpressionAtlas; Q2TBH6; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0033588; C:elongator holoenzyme complex; ISS:UniProtKB.
DR   GO; GO:0008023; C:transcription elongation factor complex; ISS:UniProtKB.
DR   GO; GO:0008607; F:phosphorylase kinase regulator activity; ISS:UniProtKB.
DR   GO; GO:0000993; F:RNA polymerase II complex binding; IEA:Ensembl.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR008728; Elongator_complex_protein_4.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR12896; PTHR12896; 1.
DR   Pfam; PF05625; PAXNEB; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Nucleus; Reference proteome; tRNA processing.
FT   CHAIN           1..423
FT                   /note="Elongator complex protein 4"
FT                   /id="PRO_0000284003"
SQ   SEQUENCE   423 AA;  46430 MW;  B9F6477A32A85409 CRC64;
     MAAAGTCGTG TRSTGSMVAA ASTNNVTSFQ RRGPRASGAD SGCSRLVSIA GTRPSVRNGQ
     LLVSTGLPAL DQLLGGGLAV GTVLLIEEDK YNIYSPLLFK YFLAEGIVSG HALLVASAKE
     DPADILQKLP APLIDDSYKK ECDEDVCSHK MEPDIKMKIA WRYQLLPKME AGPVSSSRFG
     HYYDISKRIP QELIETSKWH GFFLPEKISS AFNVESCSLT PGYMKLLQFI QNIIYKEGFD
     GSNPQKKQKN ILRIGIQNLG SPLWGDDICC TENCDTSHSL TKFLYILRGL LRTSLSACII
     TMPTHLIQNK AVIARVTNLS DTVVGLESFI GSEKETNPLY KDYHGLIHIR QIPRLNNLIY
     DISDVKDLAF KLKKKLFTIE RLHLPPDLSD TVSRSSKQDL AESTKLLGPG CGMTAGGKKH
     LDF