ID   ELP4_CAEBR              Reviewed;         333 AA.
AC   Q60ZM2; A8XRE4;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Elongator complex protein 4;
DE            Short=ELP4;
GN   Name=elpc-4; ORFNames=CBG17740;
OS   Caenorhabditis briggsae.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AF16;
RX   PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA   Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA   Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA   Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA   Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA   Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA   Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA   Durbin R.M., Waterston R.H.;
RT   "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT   genomics.";
RL   PLoS Biol. 1:166-192(2003).
CC   -!- FUNCTION: Component of the elongator complex which is required for
CC       multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl
CC       uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U
CC       (5-carbamoylmethyl uridine). The elongator complex catalyzes the
CC       formation of carboxymethyluridine in the wobble base at position 34 in
CC       tRNAs. {ECO:0000250|UniProtKB:Q96EB1}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000250|UniProtKB:Q96EB1}.
CC   -!- SUBUNIT: Component of the elongator complex.
CC       {ECO:0000250|UniProtKB:Q96EB1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q96EB1}. Nucleus
CC       {ECO:0000250|UniProtKB:Q96EB1}.
CC   -!- SIMILARITY: Belongs to the ELP4 family. {ECO:0000305}.
CC   -!- CAUTION: The elongator complex was originally thought to play a role in
CC       transcription elongation. However, it is no longer thought to play a
CC       direct role in this process and its primary function is thought to be
CC       in tRNA modification. {ECO:0000250|UniProtKB:Q96EB1}.
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DR   EMBL; HE600976; CAP35218.1; -; Genomic_DNA.
DR   RefSeq; XP_002634384.1; XM_002634338.1.
DR   AlphaFoldDB; Q60ZM2; -.
DR   SMR; Q60ZM2; -.
DR   STRING; 6238.CBG17740; -.
DR   EnsemblMetazoa; CBG17740.1; CBG17740.1; WBGene00037292.
DR   GeneID; 8576379; -.
DR   KEGG; cbr:CBG_17740; -.
DR   CTD; 8576379; -.
DR   WormBase; CBG17740; CBP10643; WBGene00037292; Cbr-elpc-4.
DR   eggNOG; KOG3949; Eukaryota.
DR   HOGENOM; CLU_031345_3_1_1; -.
DR   InParanoid; Q60ZM2; -.
DR   OMA; THNPPVD; -.
DR   OrthoDB; 973442at2759; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000008549; Chromosome IV.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0033588; C:elongator holoenzyme complex; IBA:GO_Central.
DR   GO; GO:0008023; C:transcription elongation factor complex; ISS:UniProtKB.
DR   GO; GO:0008607; F:phosphorylase kinase regulator activity; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR008728; Elongator_complex_protein_4.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR12896; PTHR12896; 1.
DR   Pfam; PF05625; PAXNEB; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Nucleus; Reference proteome; tRNA processing.
FT   CHAIN           1..333
FT                   /note="Elongator complex protein 4"
FT                   /id="PRO_0000284008"
SQ   SEQUENCE   333 AA;  37356 MW;  A6F1EF8B2F644078 CRC64;
     MLNIGDSVQI PGCSTKKRLL ETTSGCDSFD TLLGGALVNS SIVLIDEYRS RCYGSYLVRS
     FLAEGLHYGH RCFVADPSED VSETILKVIP TRKAAEDDQK KEIPSSPGQA DNDMKIAWRY
     GNVKQVTSEI GPSGNQYDFS KHLENSNVDV YNEEIPSFSG LYKILCEVVQ KEEAHTKSTG
     RGGPKKNLLR VVLKNIHLNI WEDYESLGRF LCCLRSLARS SYAVIYIVAN SHRMPNDTWR
     QLESAADTHI QLMPFNEQEK KMFRHLGTAH GYFHLKSLPR LMSAGTHTPP ILDLIFEASS
     KKGFQIRVMH LPPAFDEPAP GQQKATACQN IDF