ID   ELP4_DICDI              Reviewed;         439 AA.
AC   Q54XS0;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Elongator complex protein 4;
DE            Short=ELP4;
GN   Name=elp4; ORFNames=DDB_G0278783;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Component of the elongator complex which is required for
CC       multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl
CC       uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U
CC       (5-carbamoylmethyl uridine). The elongator complex catalyzes the
CC       formation of carboxymethyluridine in the wobble base at position 34 in
CC       tRNAs. {ECO:0000250|UniProtKB:Q96EB1}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000250|UniProtKB:Q96EB1}.
CC   -!- SUBUNIT: Component of the elongator complex.
CC       {ECO:0000250|UniProtKB:Q96EB1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q96EB1}. Nucleus
CC       {ECO:0000250|UniProtKB:Q96EB1}.
CC   -!- SIMILARITY: Belongs to the ELP4 family. {ECO:0000305}.
CC   -!- CAUTION: The elongator complex was originally thought to play a role in
CC       transcription elongation. However, it is no longer thought to play a
CC       direct role in this process and its primary function is thought to be
CC       in tRNA modification. {ECO:0000250|UniProtKB:Q96EB1}.
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DR   EMBL; AAFI02000024; EAL67993.1; -; Genomic_DNA.
DR   RefSeq; XP_641960.1; XM_636868.1.
DR   AlphaFoldDB; Q54XS0; -.
DR   SMR; Q54XS0; -.
DR   STRING; 44689.DDB0216343; -.
DR   PaxDb; Q54XS0; -.
DR   EnsemblProtists; EAL67993; EAL67993; DDB_G0278783.
DR   GeneID; 8621692; -.
DR   KEGG; ddi:DDB_G0278783; -.
DR   dictyBase; DDB_G0278783; elp4.
DR   eggNOG; KOG3949; Eukaryota.
DR   HOGENOM; CLU_031345_3_1_1; -.
DR   InParanoid; Q54XS0; -.
DR   OMA; NTTMWDD; -.
DR   PhylomeDB; Q54XS0; -.
DR   UniPathway; UPA00988; -.
DR   PRO; PR:Q54XS0; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0033588; C:elongator holoenzyme complex; IBA:GO_Central.
DR   GO; GO:0008023; C:transcription elongation factor complex; ISS:UniProtKB.
DR   GO; GO:0008607; F:phosphorylase kinase regulator activity; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR008728; Elongator_complex_protein_4.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR12896; PTHR12896; 1.
DR   Pfam; PF05625; PAXNEB; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Nucleus; Reference proteome; tRNA processing.
FT   CHAIN           1..439
FT                   /note="Elongator complex protein 4"
FT                   /id="PRO_0000328080"
FT   REGION          386..439
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   439 AA;  48530 MW;  3432765A319CB733 CRC64;
     MTTPRVPTTF TRKVAGGVKT TTTGSSLPTN NTSTTTTTTT TAAKKLPNGC KISIKNSNLL
     TSTGLTDLDD IIGGGIPIGS ILMIEEDINS SYYMFLLKYF LAEGVLQQQG VFFSSLIGID
     PFEILNKLPA RITKEEEIEA DKNDTTNNIN TNINTNNNNK QPTDELKIAW RYQQYVSNEL
     SKQQQQQSAS TIMNQTFCHS YDFTRKMNVQ SMNPELIHTL SHDAQSQAEG TSPYRNLFLE
     IQNLVYKYNK EAAMNPDQTR VLRLCIQSFS SPLWSNDEEG VIEFLHALKG LLRSSVATCV
     ISVPTYIYSG AFVKKIAHLC DTVVSINSFS GLGGETPEQF AEYLGLFNIR KIARLNTLSL
     SFHPDMLTFV FKMKRRKMCI ETIHLPPESS RAGDSKPDNN DSNKNKSDQN NIVSKMKSGS
     GLLCGGGGGG SSNNNPLDF