ID   ELP4_XENLA              Reviewed;         408 AA.
AC   Q5XG58;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Elongator complex protein 4;
GN   Name=elp4;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the elongator complex which is required for
CC       multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl
CC       uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U
CC       (5-carbamoylmethyl uridine). The elongator complex catalyzes the
CC       formation of carboxymethyluridine in the wobble base at position 34 in
CC       tRNAs. {ECO:0000250|UniProtKB:Q96EB1}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000250|UniProtKB:Q96EB1}.
CC   -!- SUBUNIT: Component of the elongator complex.
CC       {ECO:0000250|UniProtKB:Q96EB1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q96EB1}. Nucleus
CC       {ECO:0000250|UniProtKB:Q96EB1}.
CC   -!- SIMILARITY: Belongs to the ELP4 family. {ECO:0000305}.
CC   -!- CAUTION: The elongator complex was originally thought to play a role in
CC       transcription elongation. However, it is no longer thought to play a
CC       direct role in this process and its primary function is thought to be
CC       in tRNA modification. {ECO:0000250|UniProtKB:Q96EB1}.
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DR   EMBL; BC084604; AAH84604.1; -; mRNA.
DR   RefSeq; NP_001088363.1; NM_001094894.1.
DR   AlphaFoldDB; Q5XG58; -.
DR   SMR; Q5XG58; -.
DR   BioGRID; 105287; 1.
DR   IntAct; Q5XG58; 1.
DR   MaxQB; Q5XG58; -.
DR   DNASU; 495207; -.
DR   GeneID; 495207; -.
DR   KEGG; xla:495207; -.
DR   CTD; 495207; -.
DR   Xenbase; XB-GENE-991978; elp4.S.
DR   OrthoDB; 973442at2759; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000186698; Chromosome 4S.
DR   Bgee; 495207; Expressed in oocyte and 20 other tissues.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0033588; C:elongator holoenzyme complex; IEA:InterPro.
DR   GO; GO:0008023; C:transcription elongation factor complex; ISS:UniProtKB.
DR   GO; GO:0008607; F:phosphorylase kinase regulator activity; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR008728; Elongator_complex_protein_4.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR12896; PTHR12896; 1.
DR   Pfam; PF05625; PAXNEB; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Nucleus; Reference proteome; tRNA processing.
FT   CHAIN           1..408
FT                   /note="Elongator complex protein 4"
FT                   /id="PRO_0000284007"
FT   REGION          106..141
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        122..138
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   408 AA;  44865 MW;  AEE2F6E797299718 CRC64;
     MAAPCHRGVS AGDDAVGTGT SFKRKVRGKF PALPGTRPSV HNGQLLVSTG VPSLDHILGG
     GLAVGTLLLI EEDTYGTYSH LLLKYFLAEG VVSGHEVFVA SANDDPTETL QDLPSPLTDE
     VPRQNDPKRT KDTSGPADDS QEMMKIAWRY QNLPKVETLP ISSSRFGHYY DLSKTMPPEM
     SAKSHRFYLP RIMSANQKQN VSEVTCNYNQ LLESIQRVVH QEGYDGSNPQ KRPKTILRLG
     IESLGSVLWA DDICSQERPE NQHSLTRFLY GLRGLLRTSL SVCVITVPTY LIQNKAITTR
     LRSLSDTVVG LESFIGSEME ANPLYKDYHG LLHVHQIPRL NSLISDGSDT KDLAFKLKRK
     IFAIERLHLP PDLSDTVSRS SKQDLAGSAK LLSSGCGPAA GGEKHLDF