ELP5_ARATH
ID ELP5_ARATH Reviewed; 374 AA.
AC F4IQJ2; F4IQJ3; Q9ZPX3;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Elongator complex protein 5;
DE Short=AtELP5;
DE AltName: Full=Elongator component 5;
GN Name=ELP5; OrderedLocusNames=At2g18410; ORFNames=T30D6.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP SUBUNIT.
RX PubMed=20080602; DOI=10.1073/pnas.0913559107;
RA Nelissen H., De Groeve S., Fleury D., Neyt P., Bruno L., Bitonti M.B.,
RA Vandenbussche F., Van der Straeten D., Yamaguchi T., Tsukaya H.,
RA Witters E., De Jaeger G., Houben A., Van Lijsebettens M.;
RT "Plant Elongator regulates auxin-related genes during RNA polymerase II
RT transcription elongation.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:1678-1683(2010).
CC -!- FUNCTION: Component of the elongator complex which is required for
CC multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl
CC uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U
CC (5-carbamoylmethyl uridine) (By similarity). The elongator complex
CC catalyzes the formation of carboxymethyluridine in the wobble base at
CC position 34 in tRNAs (By similarity). {ECO:0000250|UniProtKB:Q8TE02}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000250|UniProtKB:Q8TE02}.
CC -!- SUBUNIT: Component of the elongator complex which consists of
CC ELP1/ELO2, ELP2, ELP3/ELO3, ELP4/ELO1, ELP5, and ELP6.
CC {ECO:0000269|PubMed:20080602}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8TE02}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q8TE02}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4IQJ2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4IQJ2-2; Sequence=VSP_042839, VSP_042840;
CC -!- SIMILARITY: Belongs to the ELP5 family. {ECO:0000305}.
CC -!- CAUTION: The elongator complex was originally thought to play a role in
CC transcription elongation. However, it is no longer thought to play a
CC direct role in this process and its primary function is thought to be
CC in tRNA modification. {ECO:0000250|UniProtKB:Q8TE02}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD15496.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006439; AAD15496.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC06766.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06767.1; -; Genomic_DNA.
DR EMBL; BX819102; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; A84564; A84564.
DR RefSeq; NP_001031371.1; NM_001036294.2. [F4IQJ2-2]
DR RefSeq; NP_179432.3; NM_127398.5. [F4IQJ2-1]
DR AlphaFoldDB; F4IQJ2; -.
DR STRING; 3702.AT2G18410.1; -.
DR iPTMnet; F4IQJ2; -.
DR PaxDb; F4IQJ2; -.
DR PRIDE; F4IQJ2; -.
DR ProteomicsDB; 222350; -. [F4IQJ2-1]
DR EnsemblPlants; AT2G18410.1; AT2G18410.1; AT2G18410. [F4IQJ2-1]
DR EnsemblPlants; AT2G18410.2; AT2G18410.2; AT2G18410. [F4IQJ2-2]
DR GeneID; 816356; -.
DR Gramene; AT2G18410.1; AT2G18410.1; AT2G18410. [F4IQJ2-1]
DR Gramene; AT2G18410.2; AT2G18410.2; AT2G18410. [F4IQJ2-2]
DR KEGG; ath:AT2G18410; -.
DR Araport; AT2G18410; -.
DR TAIR; locus:2062190; AT2G18410.
DR eggNOG; ENOG502QTQT; Eukaryota.
DR HOGENOM; CLU_060854_1_0_1; -.
DR InParanoid; F4IQJ2; -.
DR OMA; IHYIRDS; -.
DR PhylomeDB; F4IQJ2; -.
DR UniPathway; UPA00988; -.
DR PRO; PR:F4IQJ2; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4IQJ2; baseline and differential.
DR Genevisible; F4IQJ2; AT.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0033588; C:elongator holoenzyme complex; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0006400; P:tRNA modification; IBA:GO_Central.
DR GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR019519; Elp5.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR15641; PTHR15641; 1.
DR Pfam; PF10483; Elong_Iki1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Nucleus; Reference proteome;
KW tRNA processing.
FT CHAIN 1..374
FT /note="Elongator complex protein 5"
FT /id="PRO_0000416791"
FT REGION 352..374
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 360..374
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 284..306
FT /note="VQFNLQLSEKERVEKEKVVLPFE -> ASYLDLLFNYLVCVSCYNVISCS
FT (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_042839"
FT VAR_SEQ 307..374
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_042840"
SQ SEQUENCE 374 AA; 41428 MW; BE2995C3B6D1F960 CRC64;
MAESIFRKLR DGGEEGELAP ALTIEETVAS PFGLDVSGYL LTNLSSSILA GKSSSQGLVL
ITFSRSPSFY LQLLKQKGIV VSSSSKWIRI LDCYTDPLGW IDQSSTSFSE GSSLIKLHKC
VSDLKKLFSS IIEAGRELVG TGKTRFCVAI DSVNELLRHS AMPLVSGLLT DLRSHAQISS
VFWSLNTDLH QEKVTNALEY ISTMKANLEP LCPSSDGQRN ALENLFSVHQ DFGKGRFHVR
FKLRKGRVRV MSEEYHVDQS GINFSPISSV DTVIAATKSL LPKVQFNLQL SEKERVEKEK
VVLPFEHQDD GKSNEIYDGR RSLVDGKIET TPLSSMELQT DVVSSGKGGE IIYFRDSDDE
HPDSDEDPDD DLDI
