ELT2_CAEEL
ID ELT2_CAEEL Reviewed; 433 AA.
AC Q10655; Q18371;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Transcription factor elt-2 {ECO:0000305};
DE AltName: Full=GATA-type domain-containing protein elt-2 {ECO:0000305};
GN Name=elt-2 {ECO:0000312|WormBase:C33D3.1};
GN ORFNames=C33D3.1 {ECO:0000312|WormBase:C33D3.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=Bristol N2;
RX PubMed=7782329; DOI=10.1074/jbc.270.24.14666;
RA Hawkins M.G., McGhee J.D.;
RT "elt-2, a second GATA factor from the nematode Caenorhabditis elegans.";
RL J. Biol. Chem. 270:14666-14671(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=9659934; DOI=10.1016/s0012-1606(98)80006-7;
RA Fukushige T., Hawkins M.G., McGhee J.D.;
RT "The GATA-factor elt-2 is essential for formation of the Caenorhabditis
RT elegans intestine.";
RL Dev. Biol. 198:286-302(1998).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=10518545; DOI=10.1073/pnas.96.21.11883;
RA Fukushige T., Hendzel M.J., Bazett-Jones D.P., McGhee J.D.;
RT "Direct visualization of the elt-2 gut-specific GATA factor binding to a
RT target promoter inside the living Caenorhabditis elegans embryo.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:11883-11888(1999).
RN [5]
RP FUNCTION.
RX PubMed=15733671; DOI=10.1016/j.ydbio.2004.12.012;
RA Fukushige T., Goszczynski B., Yan J., McGhee J.D.;
RT "Transcriptional control and patterning of the pho-1 gene, an essential
RT acid phosphatase expressed in the C. elegans intestine.";
RL Dev. Biol. 279:446-461(2005).
RN [6]
RP FUNCTION.
RX PubMed=15734735; DOI=10.1074/jbc.m410928200;
RA Oskouian B., Mendel J., Shocron E., Lee M.A. Jr., Fyrst H., Saba J.D.;
RT "Regulation of sphingosine-1-phosphate lyase gene expression by members of
RT the GATA family of transcription factors.";
RL J. Biol. Chem. 280:18403-18410(2005).
RN [7]
RP INDUCTION BY P.AERUGINOSA.
RX PubMed=16968778; DOI=10.1073/pnas.0603424103;
RA Shapira M., Hamlin B.J., Rong J., Chen K., Ronen M., Tan M.W.;
RT "A conserved role for a GATA transcription factor in regulating epithelial
RT innate immune responses.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:14086-14091(2006).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17183709; DOI=10.1371/journal.pone.0000077;
RA Kerry S., TeKippe M., Gaddis N.C., Aballay A.;
RT "GATA transcription factor required for immunity to bacterial and fungal
RT pathogens.";
RL PLoS ONE 1:E77-E77(2006).
RN [9]
RP FUNCTION, AND INTERACTION WITH LAG-1.
RX PubMed=18003741; DOI=10.1242/dev.008680;
RA Neves A., English K., Priess J.R.;
RT "Notch-GATA synergy promotes endoderm-specific expression of ref-1 in C.
RT elegans.";
RL Development 134:4459-4468(2007).
RN [10]
RP TISSUE SPECIFICITY.
RX PubMed=17113066; DOI=10.1016/j.ydbio.2006.10.024;
RA McGhee J.D., Sleumer M.C., Bilenky M., Wong K., McKay S.J., Goszczynski B.,
RA Tian H., Krich N.D., Khattra J., Holt R.A., Baillie D.L., Kohara Y.,
RA Marra M.A., Jones S.J., Moerman D.G., Robertson A.G.;
RT "The ELT-2 GATA-factor and the global regulation of transcription in the C.
RT elegans intestine.";
RL Dev. Biol. 302:627-645(2007).
RN [11]
RP FUNCTION.
RX PubMed=17901115; DOI=10.1096/fj.06-7426com;
RA Burmeister C., Luersen K., Heinick A., Hussein A., Domagalski M.,
RA Walter R.D., Liebau E.;
RT "Oxidative stress in Caenorhabditis elegans: protective effects of the
RT Omega class glutathione transferase (GSTO-1).";
RL FASEB J. 22:343-354(2008).
RN [12]
RP FUNCTION.
RX PubMed=18024960; DOI=10.1074/jbc.m707043200;
RA Romney S.J., Thacker C., Leibold E.A.;
RT "An iron enhancer element in the FTN-1 gene directs iron-dependent
RT expression in Caenorhabditis elegans intestine.";
RL J. Biol. Chem. 283:716-725(2008).
RN [13]
RP FUNCTION, AND INTERACTION WITH PHA-4.
RX PubMed=18448117; DOI=10.1016/j.jmb.2007.11.103;
RA Anokye-Danso F., Anyanful A., Sakube Y., Kagawa H.;
RT "Transcription factors GATA/ELT-2 and forkhead/HNF-3/PHA-4 regulate the
RT tropomyosin gene expression in the pharynx and intestine of Caenorhabditis
RT elegans.";
RL J. Mol. Biol. 379:201-211(2008).
RN [14]
RP FUNCTION.
RX PubMed=19111532; DOI=10.1016/j.ydbio.2008.11.034;
RA McGhee J.D., Fukushige T., Krause M.W., Minnema S.E., Goszczynski B.,
RA Gaudet J., Kohara Y., Bossinger O., Zhao Y., Khattra J., Hirst M.,
RA Jones S.J., Marra M.A., Ruzanov P., Warner A., Zapf R., Moerman D.G.,
RA Kalb J.M.;
RT "ELT-2 is the predominant transcription factor controlling differentiation
RT and function of the C. elegans intestine, from embryo to adult.";
RL Dev. Biol. 327:551-565(2009).
RN [15]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=20807527; DOI=10.1016/j.ydbio.2010.08.020;
RA Sommermann E.M., Strohmaier K.R., Maduro M.F., Rothman J.H.;
RT "Endoderm development in Caenorhabditis elegans: the synergistic action of
RT ELT-2 and -7 mediates the specification-differentiation transition.";
RL Dev. Biol. 347:154-166(2010).
RN [16]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20498281; DOI=10.1128/mcb.01459-09;
RA Inoue H., Nishida E.;
RT "The DM domain transcription factor MAB-3 regulates male hypersensitivity
RT to oxidative stress in Caenorhabditis elegans.";
RL Mol. Cell. Biol. 30:3453-3459(2010).
RN [17]
RP DEVELOPMENTAL STAGE.
RX PubMed=20164922; DOI=10.1038/nature08781;
RA Raj A., Rifkin S.A., Andersen E., van Oudenaarden A.;
RT "Variability in gene expression underlies incomplete penetrance.";
RL Nature 463:913-918(2010).
RN [18]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20126308; DOI=10.1371/journal.pone.0009010;
RA Rohlfing A.K., Miteva Y., Hannenhalli S., Lamitina T.;
RT "Genetic and physiological activation of osmosensitive gene expression
RT mimics transcriptional signatures of pathogen infection in C. elegans.";
RL PLoS ONE 5:E9010-E9010(2010).
RN [19]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=21168435; DOI=10.1016/j.dci.2010.12.008;
RA Elliott S.L., Sturgeon C.R., Travers D.M., Montgomery M.C.;
RT "Mode of bacterial pathogenesis determines phenotype in elt-2 and elt-7
RT RNAi Caenorhabditis elegans.";
RL Dev. Comp. Immunol. 35:521-524(2011).
RN [20]
RP FUNCTION.
RX PubMed=22194696; DOI=10.1371/journal.pgen.1002394;
RA Romney S.J., Newman B.S., Thacker C., Leibold E.A.;
RT "HIF-1 regulates iron homeostasis in Caenorhabditis elegans by activation
RT and inhibition of genes involved in iron uptake and storage.";
RL PLoS Genet. 7:E1002394-E1002394(2011).
RN [21]
RP FUNCTION.
RX PubMed=22967128; DOI=10.1667/rr2989.1;
RA Kimura T., Takanami T., Sakashita T., Wada S., Kobayashi Y.,
RA Higashitani A.;
RT "Innate immune genes including a mucin-like gene, mul-1, induced by
RT ionizing radiation in Caenorhabditis elegans.";
RL Radiat. Res. 178:313-320(2012).
RN [22]
RP DEVELOPMENTAL STAGE.
RX PubMed=23863485; DOI=10.1242/dev.098012;
RA Nair G., Walton T., Murray J.I., Raj A.;
RT "Gene transcription is coordinated with, but not dependent on, cell
RT divisions during C. elegans embryonic fate specification.";
RL Development 140:3385-3394(2013).
RN [23]
RP FUNCTION, SUBCELLULAR LOCATION, UBIQUITINATION, AND DISRUPTION PHENOTYPE.
RX PubMed=23980181; DOI=10.1073/pnas.1311725110;
RA Lee S.H., Wong R.R., Chin C.Y., Lim T.Y., Eng S.A., Kong C., Ijap N.A.,
RA Lau M.S., Lim M.P., Gan Y.H., He F.L., Tan M.W., Nathan S.;
RT "Burkholderia pseudomallei suppresses Caenorhabditis elegans immunity by
RT specific degradation of a GATA transcription factor.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:15067-15072(2013).
RN [24]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=25284791; DOI=10.1016/j.celrep.2014.08.075;
RA Schieber M., Chandel N.S.;
RT "TOR signaling couples oxygen sensing to lifespan in C. elegans.";
RL Cell Rep. 9:9-15(2014).
RN [25]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24834345; DOI=10.1186/2046-2395-3-5;
RA Bansal A., Kwon E.S., Conte D. Jr., Liu H., Gilchrist M.J., MacNeil L.T.,
RA Tissenbaum H.A.;
RT "Transcriptional regulation of Caenorhabditis elegans FOXO/DAF-16 modulates
RT lifespan.";
RL Longev. Healthspan 3:5-5(2014).
RN [26]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=25340560; DOI=10.1371/journal.pgen.1004609;
RA Head B., Aballay A.;
RT "Recovery from an acute infection in C. elegans requires the GATA
RT transcription factor ELT-2.";
RL PLoS Genet. 10:E1004609-E1004609(2014).
RN [27]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=26700680; DOI=10.1242/dev.130914;
RA Wiesenfahrt T., Berg J.Y., Nishimura E.O., Robinson A.G., Goszczynski B.,
RA Lieb J.D., McGhee J.D.;
RT "The Function and Regulation of the GATA Factor ELT-2 in the C. elegans
RT Endoderm.";
RL Development 143:483-491(2016).
RN [28]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=25552416; DOI=10.1093/nar/gku1360;
RA Roh H.C., Dimitrov I., Deshmukh K., Zhao G., Warnhoff K., Cabrera D.,
RA Tsai W., Kornfeld K.;
RT "A modular system of DNA enhancer elements mediates tissue-specific
RT activation of transcription by high dietary zinc in C. elegans.";
RL Nucleic Acids Res. 43:803-816(2015).
RN [29]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=26016853; DOI=10.1371/journal.pgen.1005265;
RA Block D.H., Twumasi-Boateng K., Kang H.S., Carlisle J.A., Hanganu A.,
RA Lai T.Y., Shapira M.;
RT "The developmental intestinal regulator ELT-2 controls p38-dependent immune
RT responses in adult C. elegans.";
RL PLoS Genet. 11:E1005265-E1005265(2015).
RN [30]
RP DEVELOPMENTAL STAGE.
RX PubMed=26020930; DOI=10.1371/journal.pgen.1005268;
RA Gordon K.L., Arthur R.K., Ruvinsky I.;
RT "Phylum-level conservation of regulatory information in nematodes despite
RT extensive non-coding sequence divergence.";
RL PLoS Genet. 11:E1005268-E1005268(2015).
RN [31] {ECO:0000305}
RP FUNCTION.
RX PubMed=26963674; DOI=10.1016/j.ydbio.2016.02.031;
RA Goszczynski B., Captan V.V., Danielson A.M., Lancaster B.R., McGhee J.D.;
RT "A 44 bp intestine-specific hermaphrodite-specific enhancer from the C.
RT elegans vit-2 vitellogenin gene is directly regulated by ELT-2, MAB-3, FKH-
RT 9 and DAF-16 and indirectly regulated by the germline, by daf-2/insulin
RT signaling and by the TGF-beta/Sma/Mab pathway.";
RL Dev. Biol. 413:112-127(2016).
RN [32]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26828939; DOI=10.1371/journal.pgen.1005823;
RA Keith S.A., Maddux S.K., Zhong Y., Chinchankar M.N., Ferguson A.A.,
RA Ghazi A., Fisher A.L.;
RT "Graded proteasome dysfunction in Caenorhabditis elegans activates an
RT adaptive response involving the conserved skn-1 and elt-2 transcription
RT factors and the autophagy-lysosome pathway.";
RL PLoS Genet. 12:E1005823-E1005823(2016).
RN [33]
RP FUNCTION, INTERACTION WITH RPT-6, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=30265660; DOI=10.1371/journal.pgen.1007693;
RA Olaitan A.O., Aballay A.;
RT "Non-proteolytic activity of 19S proteasome subunit RPT-6 regulates GATA
RT transcription during response to infection.";
RL PLoS Genet. 14:E1007693-E1007693(2018).
CC -!- FUNCTION: Transcriptional activator that binds to the consensus
CC sequence 5'-[AT]GATA[AG]-3' (PubMed:7782329, PubMed:15733671,
CC PubMed:18003741, PubMed:18024960, PubMed:18448117, PubMed:26700680,
CC PubMed:26016853, PubMed:26963674). Predominantly directs the
CC transcription of intestinal genes such as ges-1, cpr-6, pho-1, ftn-1,
CC vit-2 and lev-11, and itself (PubMed:9659934, PubMed:10518545,
CC PubMed:15733671, PubMed:18448117, PubMed:19111532, PubMed:26700680,
CC PubMed:26963674). Required for gut-specific differentiation,
CC specifically acting with the GATA region-binding transcription factor
CC elt-7 to control normal gene expression and promote normal formation of
CC the intestine (PubMed:20807527). Regulates intestinal gene expression
CC in response to hypoxia to promote longevity (PubMed:25284791).
CC Modulation of longevity may, in part, be the result of regulation of
CC expression of daf-16 isoforms d and f in the intestine
CC (PubMed:24834345). Regulates tissue specific gene expression at basal
CC levels and in response to bacterial infection in the intestine to
CC control innate immunity (PubMed:16968778, PubMed:21168435,
CC PubMed:25340560, PubMed:26016853). Plays a role in the induction of
CC metal-responsive genes, activating gene expression from zinc-activated
CC promoters and iron-dependent promoters and enhancers (PubMed:18024960,
CC PubMed:22194696, PubMed:25552416). May regulate the expression of genes
CC that control sensitivity to oxidative stress, in a mab-3-dependent
CC manner, and osmotic stress, in conjunction with the GATA region-binding
CC transcription factor elt-3 (PubMed:17901115, PubMed:20498281,
CC PubMed:20126308). May play a role in sphingolipid signaling by
CC regulating the expression of the sphingosine-1-phosphate degrading
CC enzyme, sphingosine-1-phosphate lyase (PubMed:15734735). May act with
CC the Notch signaling pathway to promote endodermal gene expression
CC (PubMed:18003741). Has a protective role in response to infection by
CC Gram-negative bacteria such as S.enterica, E.coli, P.aeruginosa and
CC B.pseudomallei, Gram-positive bacterium E.faecalis and fungal pathogen
CC C.neoformans (PubMed:16968778, PubMed:17183709, PubMed:21168435,
CC PubMed:23980181, PubMed:30265660). An association with the 26S
CC proteasome regulatory subunit rpt-6, in part, controls gene expression
CC in response to infection by P.aeruginosa (PubMed:30265660). Regulates
CC gene expression during the recovery phase following a bacterial
CC infection (PubMed:25340560, PubMed:30265660). May act with p38-
CC activated transcription factors to control p38 gene induction in
CC response to bacterial infection (PubMed:22967128, PubMed:26016853).
CC Controls lysosome formation in the intestine by controlling lysosomal
CC gene expression (PubMed:26828939). {ECO:0000269|PubMed:10518545,
CC ECO:0000269|PubMed:15733671, ECO:0000269|PubMed:15734735,
CC ECO:0000269|PubMed:16968778, ECO:0000269|PubMed:17183709,
CC ECO:0000269|PubMed:17901115, ECO:0000269|PubMed:18003741,
CC ECO:0000269|PubMed:18024960, ECO:0000269|PubMed:18448117,
CC ECO:0000269|PubMed:19111532, ECO:0000269|PubMed:20126308,
CC ECO:0000269|PubMed:20498281, ECO:0000269|PubMed:20807527,
CC ECO:0000269|PubMed:21168435, ECO:0000269|PubMed:22194696,
CC ECO:0000269|PubMed:22967128, ECO:0000269|PubMed:23980181,
CC ECO:0000269|PubMed:25284791, ECO:0000269|PubMed:25340560,
CC ECO:0000269|PubMed:26016853, ECO:0000269|PubMed:26700680,
CC ECO:0000269|PubMed:26828939, ECO:0000269|PubMed:30265660,
CC ECO:0000269|PubMed:7782329}.
CC -!- SUBUNIT: Interacts with lag-1 (PubMed:18003741). Interacts with pha-4
CC (PubMed:18448117). Interacts with rpt-6 (PubMed:30265660).
CC {ECO:0000269|PubMed:18003741, ECO:0000269|PubMed:18448117,
CC ECO:0000269|PubMed:30265660}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10518545,
CC ECO:0000269|PubMed:23980181, ECO:0000269|PubMed:30265660,
CC ECO:0000269|PubMed:7782329, ECO:0000269|PubMed:9659934}. Note=Expressed
CC in discrete foci within nuclei of the gut in embryos.
CC {ECO:0000269|PubMed:10518545}.
CC -!- TISSUE SPECIFICITY: Expressed in the intestine.
CC {ECO:0000269|PubMed:17113066, ECO:0000269|PubMed:26016853}.
CC -!- DEVELOPMENTAL STAGE: Expressed at all stages but more highly during
CC embryogenesis, with expression beginning at the 2E cell stage
CC (endodermal stage). Expression continues to adulthood.
CC {ECO:0000269|PubMed:10518545, ECO:0000269|PubMed:20164922,
CC ECO:0000269|PubMed:20807527, ECO:0000269|PubMed:23863485,
CC ECO:0000269|PubMed:26020930, ECO:0000269|PubMed:26700680,
CC ECO:0000269|PubMed:7782329, ECO:0000269|PubMed:9659934}.
CC -!- INDUCTION: By P.aeruginosa infection. {ECO:0000269|PubMed:16968778}.
CC -!- PTM: May be ubiquitinated in response to infection by B.pseudomallei.
CC {ECO:0000269|PubMed:23980181}.
CC -!- DISRUPTION PHENOTYPE: Animals do not survive for long beyond hatching
CC and frequently die at the L1 larval stage (PubMed:9659934,
CC PubMed:20807527). Upon attempts to feed, food accumulates behind the
CC pharynx and there is blockage of the brush border that surrounds the
CC gut lumen in surviving L1 stage larvae (PubMed:9659934,
CC PubMed:20807527). RNAi-mediated knockdown results in a reduced body
CC size and reduced gene expression from zinc-activated promoters and of
CC genes related to the immune response (PubMed:25552416,
CC PubMed:26016853). This reduction in target gene expression is enhanced
CC when infected with B. pseudomallei (PubMed:23980181). RNAi-mediated
CC knockdown results in increased sensitivity and mortality when exposed
CC to Gram-negative bacteria such as S.enterica, E.coli, P.aeruginosa and
CC B.pseudomallei, Gram-positive bacterium E.faecalis and fungal pathogen
CC C.neoformans (PubMed:16968778, PubMed:17183709, PubMed:21168435,
CC PubMed:23980181, PubMed:30265660). Furthermore, when exposed to E.coli
CC and P.aeruginosa, the intestines distend due to the colonization and
CC proliferation of its bacterial content (PubMed:16968778,
CC PubMed:17183709, PubMed:21168435). RNAi-mediated knockdown at the L4
CC larval stage, in addition, prevents the recovery of P.aeruginosa
CC infected animals treated with the antibiotic streptomycin
CC (PubMed:30265660). RNAi-mediated knockdown also results in increased
CC sensitivity to heat and to arsenite- and paraquat-induced oxidative
CC stress (PubMed:16968778, PubMed:20498281). RNAi-mediated knockdown
CC results in fewer intestinal lysosomes (PubMed:26828939). RNAi-mediated
CC knockdown during larval development results in smaller animals
CC (PubMed:26016853). RNAi-mediated knockdown specifically during the L4
CC larval stage inhibits the expression of intestinal genes such as gsto-
CC 1, which is normally induced under hypoxic conditions, prevents
CC increased longevity induced by transient hypoxia exposure and prevents
CC mitohormesis when exposed to the mitochondrial reactive oxygen species-
CC generating agent paraquat (PubMed:25284791). In addition, RNAi-mediated
CC knockdown at this stage prevents the expression of genes that are
CC usually up-regulated during recovery in response to tetracycline or
CC kanamycin treatment following an infection and furthermore, prevents
CC the recovery of S. enterica infected animals treated with tetracycline
CC (PubMed:25340560). RNAi-mediated knockdown on an elt-3 knockout
CC background results in increased sensitivity to osmotic stress
CC (PubMed:20126308). Double mutation with elt-7 results in arrest at the
CC L1 stage of larval development, reduced expression of gut-specific
CC genes and a severe disruption to normal gut differentiation with the
CC absence of birefringent and rhabditin granules, which are
CC characteristic of normal gut differentiation, largely at the regions of
CC the cell that interface with the pharyngeal and rectal valves
CC (PubMed:20807527). These mutants also have essentially no gut lumen,
CC with the infrequent occurance of patches of lumen and brush border in
CC few animals, and reduced gut epithelialization as indicated by reduced
CC expression of epithelial markers erm-1b, itx-1 and ajm-1
CC (PubMed:20807527). RNAi-mediated knockdown specifically during the L4
CC larval stage reduces the expression of daf-16 isoforms d and f, but has
CC little or effect on isoform a. {ECO:0000269|PubMed:16968778,
CC ECO:0000269|PubMed:17183709, ECO:0000269|PubMed:20126308,
CC ECO:0000269|PubMed:20498281, ECO:0000269|PubMed:20807527,
CC ECO:0000269|PubMed:21168435, ECO:0000269|PubMed:23980181,
CC ECO:0000269|PubMed:24834345, ECO:0000269|PubMed:25284791,
CC ECO:0000269|PubMed:25340560, ECO:0000269|PubMed:25552416,
CC ECO:0000269|PubMed:26016853, ECO:0000269|PubMed:26828939,
CC ECO:0000269|PubMed:30265660, ECO:0000269|PubMed:9659934}.
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DR EMBL; U25175; AAC36130.1; -; Genomic_DNA.
DR EMBL; BX284606; CAA90029.2; -; Genomic_DNA.
DR PIR; A56953; A56953.
DR PIR; T19677; T19677.
DR RefSeq; NP_509755.2; NM_077354.4.
DR AlphaFoldDB; Q10655; -.
DR SMR; Q10655; -.
DR BioGRID; 46163; 18.
DR IntAct; Q10655; 2.
DR STRING; 6239.C33D3.1; -.
DR iPTMnet; Q10655; -.
DR EPD; Q10655; -.
DR PaxDb; Q10655; -.
DR PeptideAtlas; Q10655; -.
DR EnsemblMetazoa; C33D3.1.1; C33D3.1.1; WBGene00001250.
DR GeneID; 181250; -.
DR KEGG; cel:CELE_C33D3.1; -.
DR UCSC; C33D3.1; c. elegans.
DR CTD; 181250; -.
DR WormBase; C33D3.1; CE31430; WBGene00001250; elt-2.
DR eggNOG; KOG1601; Eukaryota.
DR GeneTree; ENSGT00970000196325; -.
DR HOGENOM; CLU_644427_0_0_1; -.
DR InParanoid; Q10655; -.
DR OMA; NIQVHVM; -.
DR OrthoDB; 1183743at2759; -.
DR Reactome; R-CEL-5683826; Surfactant metabolism.
DR PRO; PR:Q10655; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00001250; Expressed in E lineage cell (C elegans) and 18 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:WormBase.
DR GO; GO:0003690; F:double-stranded DNA binding; IDA:WormBase.
DR GO; GO:0004857; F:enzyme inhibitor activity; IDA:WormBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0045165; P:cell fate commitment; IBA:GO_Central.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IMP:UniProtKB.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:UniProtKB.
DR GO; GO:0007586; P:digestion; IMP:WormBase.
DR GO; GO:0007492; P:endoderm development; IMP:WormBase.
DR GO; GO:0045087; P:innate immune response; IMP:WormBase.
DR GO; GO:0043086; P:negative regulation of catalytic activity; IDA:WormBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0002119; P:nematode larval development; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:WormBase.
DR GO; GO:1902097; P:positive regulation of transcription from RNA polymerase II promoter involved in defense response to Gram-negative bacterium; IMP:WormBase.
DR GO; GO:0030856; P:regulation of epithelial cell differentiation; IMP:WormBase.
DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR CDD; cd00202; ZnF_GATA; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR039355; Transcription_factor_GATA.
DR InterPro; IPR000679; Znf_GATA.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR PANTHER; PTHR10071; PTHR10071; 1.
DR Pfam; PF00320; GATA; 1.
DR PRINTS; PR00619; GATAZNFINGER.
DR SMART; SM00401; ZnF_GATA; 1.
DR PROSITE; PS00344; GATA_ZN_FINGER_1; 1.
DR PROSITE; PS50114; GATA_ZN_FINGER_2; 1.
PE 1: Evidence at protein level;
KW Activator; Differentiation; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation;
KW Ubl conjugation; Zinc; Zinc-finger.
FT CHAIN 1..433
FT /note="Transcription factor elt-2"
FT /evidence="ECO:0000305"
FT /id="PRO_0000083472"
FT ZN_FING 237..261
FT /note="GATA-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00094"
FT REGION 1..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 194..235
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 275..332
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..45
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 207..235
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 275..292
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 299..313
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 433 AA; 47116 MW; 59C2DEB0753E5499 CRC64;
MDNNYNDNVN GWAEMEPSQP MGGLRLPTQN MDPPEQNNES QLSELPRMKI DNDYASPIER
QSVITSGTNN YEPKVETVTS FFHTGIDYSN FGMLDQTTMQ PFYPLYSGIP VNTLGTFSGY
TNSIYDKPSL YDPSIPTINI PSTYPTVAPT YECVKCSQSC GAGMKAVNGG MMCVNCSTPK
TTYSPPVAYS TSLGQPPILE IPSEQPTAKI AKQSSKKSSS SNRGSNGSAS RRQGLVCSNC
NGTNTTLWRR NAEGDPVCNA CGLYFKLHHI PRPTSMKKEG ALQTRKRKSK SGDSSTPSTS
RARERKFERA SSSTEKAQRS SNRRAGSAKA DRELSTAAVA AATATYVSHA DLYPVSSAAV
TLPDQTYSNY YQWNTAATAG LMMVPNDQNY VYAATNYQTG LRPADNIQVH VMPVQDDETK
AAARDLEAVD GDS
