ELT7_CAEEL
ID ELT7_CAEEL Reviewed; 198 AA.
AC O61924; A6PVB2;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Transcription factor elt-7 {ECO:0000305};
GN Name=elt-7 {ECO:0000312|WormBase:C18G1.2a};
GN ORFNames=C18G1.2 {ECO:0000312|WormBase:C18G1.2a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP INDUCTION BY P.AERUGINOSA.
RX PubMed=16968778; DOI=10.1073/pnas.0603424103;
RA Shapira M., Hamlin B.J., Rong J., Chen K., Ronen M., Tan M.W.;
RT "A conserved role for a GATA transcription factor in regulating epithelial
RT innate immune responses.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:14086-14091(2006).
RN [3] {ECO:0000305}
RP DISRUPTION PHENOTYPE.
RX PubMed=18448117; DOI=10.1016/j.jmb.2007.11.103;
RA Anokye-Danso F., Anyanful A., Sakube Y., Kagawa H.;
RT "Transcription factors GATA/ELT-2 and forkhead/HNF-3/PHA-4 regulate the
RT tropomyosin gene expression in the pharynx and intestine of Caenorhabditis
RT elegans.";
RL J. Mol. Biol. 379:201-211(2008).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=20807527; DOI=10.1016/j.ydbio.2010.08.020;
RA Sommermann E.M., Strohmaier K.R., Maduro M.F., Rothman J.H.;
RT "Endoderm development in Caenorhabditis elegans: the synergistic action of
RT ELT-2 and -7 mediates the specification-differentiation transition.";
RL Dev. Biol. 347:154-166(2010).
RN [5] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=21168435; DOI=10.1016/j.dci.2010.12.008;
RA Elliott S.L., Sturgeon C.R., Travers D.M., Montgomery M.C.;
RT "Mode of bacterial pathogenesis determines phenotype in elt-2 and elt-7
RT RNAi Caenorhabditis elegans.";
RL Dev. Comp. Immunol. 35:521-524(2011).
RN [6] {ECO:0000305}
RP FUNCTION.
RX PubMed=24257624; DOI=10.1242/dev.103010;
RA Riddle M.R., Weintraub A., Nguyen K.C., Hall D.H., Rothman J.H.;
RT "Transdifferentiation and remodeling of post-embryonic C. elegans cells by
RT a single transcription factor.";
RL Development 140:4844-4849(2013).
RN [7]
RP FUNCTION.
RX PubMed=26700680; DOI=10.1242/dev.130914;
RA Wiesenfahrt T., Berg J.Y., Nishimura E.O., Robinson A.G., Goszczynski B.,
RA Lieb J.D., McGhee J.D.;
RT "The Function and Regulation of the GATA Factor ELT-2 in the C. elegans
RT Endoderm.";
RL Development 143:483-491(2016).
CC -!- FUNCTION: Transcriptional activator that binds to the consensus
CC sequence 5'-[AT]GATA[AG]-3' (PubMed:26700680). Required for gut-
CC specific differentiation, specifically acting with the GATA region-
CC binding transcription factor elt-2 to control normal gene expression
CC and promote normal formation of the intestine (PubMed:20807527,
CC PubMed:24257624). May have a protective role in response to infection
CC by Gram-negative bacteria such as P.aeruginosa (PubMed:21168435).
CC {ECO:0000269|PubMed:20807527, ECO:0000269|PubMed:21168435,
CC ECO:0000269|PubMed:24257624, ECO:0000269|PubMed:26700680}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:20807527}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:C18G1.2a};
CC IsoId=O61924-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:C18G1.2b};
CC IsoId=O61924-2; Sequence=VSP_058156;
CC -!- DEVELOPMENTAL STAGE: Expressed highly during embryogenesis. Expressed
CC in the endoderm in cells of the gut lineage beginning at the 2E cell
CC stage (endodermal cell stage) of embryogenesis through to adulthood.
CC {ECO:0000269|PubMed:20807527}.
CC -!- INDUCTION: Induced by P.aeruginosa infection.
CC {ECO:0000269|PubMed:16968778}.
CC -!- DISRUPTION PHENOTYPE: No obvious phenotype (PubMed:20807527). RNAi-
CC mediated knockdown results in no obvious phenotype (PubMed:18448117).
CC Following infection with P.aeruginosa, RNAi-mediated knockdown results
CC in reduced survival and slight intestinal distension (PubMed:21168435).
CC Double mutation with elt-2 results in arrest at the L1 stage of larval
CC development, reduced expression of gut-specific genes and a severe
CC disruption to normal gut differentiation with the absence of
CC birefringent and rhabditin granules, which are characteristic of normal
CC gut differentiation, largely at the regions of the cell that interface
CC with the pharyngeal and rectal valves (PubMed:20807527). These mutants
CC also have essentially no gut lumen, with only infrequent patches of
CC lumen and brush border in few animals, and reduced gut
CC epithelialization as indicated by reduced expression of epithelial
CC markers erm-1b, itx-1 and ajm-1 (PubMed:20807527).
CC {ECO:0000269|PubMed:18448117, ECO:0000269|PubMed:20807527,
CC ECO:0000269|PubMed:21168435}.
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DR EMBL; BX284605; CCD65225.1; -; Genomic_DNA.
DR EMBL; BX284605; CCD65231.1; -; Genomic_DNA.
DR PIR; T33255; T33255.
DR RefSeq; NP_001122856.1; NM_001129384.3.
DR RefSeq; NP_504283.1; NM_071882.5. [O61924-1]
DR AlphaFoldDB; O61924; -.
DR IntAct; O61924; 22.
DR STRING; 6239.C18G1.2a; -.
DR PaxDb; O61924; -.
DR EnsemblMetazoa; C18G1.2a.1; C18G1.2a.1; WBGene00015981. [O61924-1]
DR EnsemblMetazoa; C18G1.2b.1; C18G1.2b.1; WBGene00015981. [O61924-2]
DR GeneID; 178868; -.
DR KEGG; cel:CELE_C18G1.2; -.
DR UCSC; C18G1.2a; c. elegans.
DR CTD; 178868; -.
DR WormBase; C18G1.2a; CE17418; WBGene00015981; elt-7. [O61924-1]
DR WormBase; C18G1.2b; CE41205; WBGene00015981; elt-7. [O61924-2]
DR eggNOG; KOG1601; Eukaryota.
DR HOGENOM; CLU_120635_0_0_1; -.
DR InParanoid; O61924; -.
DR OMA; LECNACN; -.
DR OrthoDB; 1480372at2759; -.
DR PhylomeDB; O61924; -.
DR SignaLink; O61924; -.
DR PRO; PR:O61924; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00015981; Expressed in embryo and 4 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; HEP:WormBase.
DR GO; GO:0007493; P:endodermal cell fate determination; IGI:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR CDD; cd00202; ZnF_GATA; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR039355; Transcription_factor_GATA.
DR InterPro; IPR000679; Znf_GATA.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR PANTHER; PTHR10071; PTHR10071; 1.
DR Pfam; PF00320; GATA; 1.
DR PRINTS; PR00619; GATAZNFINGER.
DR SMART; SM00401; ZnF_GATA; 1.
DR PROSITE; PS00344; GATA_ZN_FINGER_1; 1.
DR PROSITE; PS50114; GATA_ZN_FINGER_2; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; Differentiation; DNA-binding;
KW Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..198
FT /note="Transcription factor elt-7"
FT /evidence="ECO:0000305"
FT /id="PRO_0000435724"
FT ZN_FING 143..167
FT /note="GATA-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00094"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..82
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_058156"
SQ SEQUENCE 198 AA; 23097 MW; FA877E99888DD46D CRC64;
MLPETTTLQP LPSVTTIMNE PPARSPMEDY YLFNQFQYNQ NPYVYPPQPV YYNTWQDAST
HHFDPFQSYQ IPSPYEQPIQ SSMCTTPLQP LEDSRIIFDE SLTKNENEQK SFVEQDSSYE
SSGNRFGSQK GKKIAKVIRD ACCSHCSTTT TTLWRKNDEG NLECNACNLY YRHNKVKRPL
SLCKQKPTTR KRRQAKKE
