ELTB_CLOPF
ID ELTB_CLOPF Reviewed; 319 AA.
AC P01558; O32373; O52925; Q05130; Q46182; Q79AH6; Q79DV9; Q79DW4; Q79DX2;
AC Q8GNM6; Q9R457;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Heat-labile enterotoxin B chain;
DE Flags: Precursor;
GN Name=cpe;
OS Clostridium perfringens.
OG Plasmid pF4969.
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1502;
RN [1]
RP PROTEIN SEQUENCE.
RC STRAIN=Type A;
RX PubMed=3920076; DOI=10.1016/0014-5793(85)80358-6;
RA Richardson M., Granum P.E.;
RT "The amino acid sequence of the enterotoxin from Clostridium perfringens
RT type A.";
RL FEBS Lett. 182:479-484(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2802575; DOI=10.1007/bf00399981;
RA Van Damme-Jongsten M., Wernars K., Notermans S.;
RT "Cloning and sequencing of the Clostridium perfringens enterotoxin gene.";
RL Antonie Van Leeuwenhoek 56:181-190(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 12917 / NCTC 8239 / Type A;
RX PubMed=8335373; DOI=10.1128/iai.61.8.3429-3439.1993;
RA Czeczulin J.R., Hanna P.C., McClane B.A.;
RT "Cloning, nucleotide sequencing, and expression of the Clostridium
RT perfringens enterotoxin gene in Escherichia coli.";
RL Infect. Immun. 61:3429-3439(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 12917 / NCTC 8239 / Type A;
RX PubMed=8162194; DOI=10.1099/13500872-140-1-97;
RA Brynestad S., Iwanejko L.A., Stewart G.S., Granum P.E.;
RT "A complex array of Hpr consensus DNA recognition sequences proximal to the
RT enterotoxin gene in Clostridium perfringens type A.";
RL Microbiology 140:97-104(1994).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=8-6 / Type A;
RX PubMed=7783636; DOI=10.1111/j.1365-2958.1995.tb02373.x;
RA Cornillot E., Saint-Joanis B., Daube G., Katayama S., Granum P.E.,
RA Canard B., Cole S.T.;
RT "The enterotoxin gene (cpe) of Clostridium perfringens can be chromosomal
RT or plasmid-borne.";
RL Mol. Microbiol. 15:639-647(1995).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=F3686 / Type A;
RA Brynestad S., Synstad B., Granum P.E.;
RT "Genomic positioning is important for the expression of the enterotoxin in
RT Clostridium perfringens.";
RL Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=B3550.5;
RX PubMed=9593490; DOI=10.1017/s0950268897008534;
RA Netherwood T., Binns M., Townsend H., Wood J.L.N., Mumford J.A.,
RA Chanter N.;
RT "Clostridium perfringens enterotoxin from equine isolates; its
RT characterisation, sequence and role in foal diarrhoea.";
RL Epidemiol. Infect. 120:193-200(1998).
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=F4969 / Type A; PLASMID=pF4969;
RX PubMed=12117935; DOI=10.1128/iai.70.8.4261-4272.2002;
RA Miyamoto K., Chakrabarti G., Morino Y., McClane B.A.;
RT "Organization of the plasmid cpe Locus in Clostridium perfringens type A
RT isolates.";
RL Infect. Immun. 70:4261-4272(2002).
RN [9]
RP PROTEIN SEQUENCE OF 1-63, AND CLEAVAGE.
RC STRAIN=Type A;
RX PubMed=6303961; DOI=10.1128/iai.40.3.943-949.1983;
RA Richardson M., Granum P.E.;
RT "Sequence of the amino-terminal part of enterotoxin from Clostridium
RT perfringens type A: identification of points of trypsin activation.";
RL Infect. Immun. 40:943-949(1983).
RN [10]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
RA Iwanejko L.A., Stewart G.S.A.B.;
RT "Cloning in Escherichia coli of a DNA sequence with translational fidelity
RT to the known protein sequence for Clostridium perfringens sporulation
RT specific enterotoxin.";
RL Biochem. Soc. Trans. 16:1069-1069(1988).
RN [11]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
RC STRAIN=ATCC 12917 / NCTC 8239 / Type A;
RX PubMed=2557378; DOI=10.1099/00221287-135-4-903;
RA Iwanejko L.A., Routledge M.N., Stewart G.S.;
RT "Cloning in Escherichia coli of the enterotoxin gene from Clostridium
RT perfringens type A.";
RL J. Gen. Microbiol. 135:903-909(1989).
RN [12]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
RC STRAIN=ATCC 12917 / NCTC 8239 / Type A, NCTC 10240, and NCTC 8798 / Type A;
RX PubMed=7960138; DOI=10.1128/iai.62.12.5550-5558.1994;
RA Melville S.B., Labbe R., Sonenshein A.L.;
RT "Expression from the Clostridium perfringens cpe promoter in C. perfringens
RT and Bacillus subtilis.";
RL Infect. Immun. 62:5550-5558(1994).
RN [13]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 171-236 AND 290-319.
RC STRAIN=ATCC 12917 / NCTC 8239 / Type A;
RX PubMed=2556374; DOI=10.1128/jb.171.12.6815-6820.1989;
RA Hanna P.C., Wnek A.P., McClane B.A.;
RT "Molecular cloning of the 3' half of the Clostridium perfringens
RT enterotoxin gene and demonstration that this region encodes receptor-
RT binding activity.";
RL J. Bacteriol. 171:6815-6820(1989).
RN [14] {ECO:0007744|PDB:3X29}
RP X-RAY CRYSTALLOGRAPHY (3.70 ANGSTROMS) OF 203-319 IN COMPLEX WITH HUMAN
RP CLDN19, INTERACTION WITH HUMAN CLDN19, AND FUNCTION.
RX PubMed=25678664; DOI=10.1126/science.1261833;
RA Saitoh Y., Suzuki H., Tani K., Nishikawa K., Irie K., Ogura Y., Tamura A.,
RA Tsukita S., Fujiyoshi Y.;
RT "Tight junctions. Structural insight into tight junction disassembly by
RT Clostridium perfringens enterotoxin.";
RL Science 347:775-778(2015).
RN [15] {ECO:0007744|PDB:5B2G}
RP X-RAY CRYSTALLOGRAPHY (3.50 ANGSTROMS) OF 187-319 IN COMPLEX WITH HUMAN
RP CLDN4, INTERACTION WITH HUMAN CLDN4, AND MUTAGENESIS OF LEU-223 AND
RP LEU-315.
RX PubMed=27647526; DOI=10.1038/srep33632;
RA Shinoda T., Shinya N., Ito K., Ohsawa N., Terada T., Hirata K., Kawano Y.,
RA Yamamoto M., Kimura-Someya T., Yokoyama S., Shirouzu M.;
RT "Structural basis for disruption of claudin assembly in tight junctions by
RT an enterotoxin.";
RL Sci. Rep. 6:33632-33632(2016).
CC -!- FUNCTION: This enterotoxin is responsible for many cases of a mild type
CC of food poisoning. {ECO:0000305|PubMed:25678664}.
CC -!- SUBUNIT: Interacts with host CLDN4 and CLDN19; the interaction disrupts
CC host claudin assembly in tight junctions. {ECO:0000269|PubMed:25678664,
CC ECO:0000269|PubMed:27647526}.
CC -!- INTERACTION:
CC P01558; Q9ET38: Cldn19; Xeno; NbExp=2; IntAct=EBI-16142958, EBI-8036280;
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M98037; AAA72120.1; -; Unassigned_DNA.
DR EMBL; X71844; CAA50691.1; -; Genomic_DNA.
DR EMBL; X81849; CAA57443.1; -; Genomic_DNA.
DR EMBL; Y16009; CAA75931.1; -; Genomic_DNA.
DR EMBL; AJ000766; CAA04327.1; -; Genomic_DNA.
DR EMBL; AF416450; AAM12505.1; -; Genomic_DNA.
DR EMBL; AF511071; AAM50524.2; -; Genomic_DNA.
DR EMBL; M27733; AAA58723.1; -; Genomic_DNA.
DR EMBL; M25259; AAA57125.1; -; Genomic_DNA.
DR EMBL; U11257; AAA61569.1; -; Genomic_DNA.
DR EMBL; U11294; AAA61570.1; -; Genomic_DNA.
DR EMBL; U11259; AAA61571.1; -; Genomic_DNA.
DR EMBL; M31795; AAA23234.1; -; Genomic_DNA.
DR EMBL; M63880; AAA66245.1; -; Genomic_DNA.
DR PIR; A37183; QLCLBF.
DR RefSeq; WP_003470007.1; NZ_UFXM01000001.1.
DR RefSeq; YP_473394.1; NC_007772.1.
DR RefSeq; YP_473485.1; NC_007773.1.
DR PDB; 2QUO; X-ray; 1.75 A; A=194-319.
DR PDB; 2XH6; X-ray; 2.69 A; A/B/C=1-319.
DR PDB; 2YHJ; X-ray; 4.00 A; A/B=1-319.
DR PDB; 3AM2; X-ray; 2.51 A; A=1-319.
DR PDB; 3X29; X-ray; 3.70 A; B/D=203-319.
DR PDB; 3ZIW; X-ray; 1.90 A; A/B/C/D/E/F=38-319.
DR PDB; 3ZIX; X-ray; 1.90 A; A/B/C/D/E/F=38-319.
DR PDB; 4P5H; X-ray; 3.38 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O=38-319.
DR PDB; 5B2G; X-ray; 3.50 A; B/D/F/H=187-319.
DR PDB; 6AKE; X-ray; 3.60 A; B/D=203-319.
DR PDB; 6AKF; X-ray; 3.90 A; B/D/F/H=203-319.
DR PDB; 6AKG; X-ray; 4.30 A; B/D=203-319.
DR PDB; 6OV2; X-ray; 3.20 A; B=194-319.
DR PDB; 6OV3; X-ray; 3.25 A; B=194-319.
DR PDB; 7KP4; X-ray; 3.37 A; B=192-319.
DR PDB; 7TDM; EM; 6.90 A; B=192-319.
DR PDB; 7TDN; EM; 5.00 A; B=192-319.
DR PDBsum; 2QUO; -.
DR PDBsum; 2XH6; -.
DR PDBsum; 2YHJ; -.
DR PDBsum; 3AM2; -.
DR PDBsum; 3X29; -.
DR PDBsum; 3ZIW; -.
DR PDBsum; 3ZIX; -.
DR PDBsum; 4P5H; -.
DR PDBsum; 5B2G; -.
DR PDBsum; 6AKE; -.
DR PDBsum; 6AKF; -.
DR PDBsum; 6AKG; -.
DR PDBsum; 6OV2; -.
DR PDBsum; 6OV3; -.
DR PDBsum; 7KP4; -.
DR PDBsum; 7TDM; -.
DR PDBsum; 7TDN; -.
DR AlphaFoldDB; P01558; -.
DR SMR; P01558; -.
DR DIP; DIP-61488N; -.
DR IntAct; P01558; 2.
DR TCDB; 1.C.59.1.1; the clostridium perfringens enterotoxin (cpe) family.
DR EvolutionaryTrace; P01558; -.
DR PRO; PR:P01558; -.
DR GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR003897; Clenterotox.
DR Pfam; PF03505; Clenterotox; 1.
DR PRINTS; PR01394; CLENTEROTOXN.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Enterotoxin; Plasmid; Toxin;
KW Virulence.
FT PROPEP 1..25
FT /note="Activation peptide"
FT /id="PRO_0000292863"
FT CHAIN 26..319
FT /note="Heat-labile enterotoxin B chain"
FT /id="PRO_0000197824"
FT REGION 26..31
FT /note="Membrane-binding"
FT /evidence="ECO:0000255"
FT SITE 1
FT /note="Not N-formylated"
FT SITE 25..26
FT /note="Cleavage; by trypsin"
FT MUTAGEN 223
FT /note="L->A: No effect on interaction with human CLDN4."
FT /evidence="ECO:0000269|PubMed:27647526"
FT MUTAGEN 223
FT /note="L->D: Strongly decreases interaction with human
FT CLDN4."
FT /evidence="ECO:0000269|PubMed:27647526"
FT MUTAGEN 315
FT /note="L->A: No effect on interaction with human CLDN4."
FT /evidence="ECO:0000269|PubMed:27647526"
FT MUTAGEN 315
FT /note="L->D: Strongly decreases interaction with human
FT CLDN4."
FT /evidence="ECO:0000269|PubMed:27647526"
FT CONFLICT 2
FT /note="L -> V (in Ref. 7; CAA04327)"
FT /evidence="ECO:0000305"
FT CONFLICT 32
FT /note="N -> NNNL (in Ref. 1; AA sequence and 9; AA
FT sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 50
FT /note="W -> L (in Ref. 1; AA sequence and 9; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 52
FT /note="L -> I (in Ref. 9; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 58
FT /note="V -> I (in Ref. 9; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 58
FT /note="V -> L (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 86..94
FT /note="NFSVGFTSE -> VG (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 103..112
FT /note="GFGITIGEQN -> FTSEF (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 111
FT /note="Q -> E (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 114
FT /note="I -> T (in Ref. 6; CAA75931)"
FT /evidence="ECO:0000305"
FT CONFLICT 152
FT /note="D -> S (in Ref. 5; CAA57443)"
FT /evidence="ECO:0000305"
FT CONFLICT 164
FT /note="S -> M (in Ref. 5; CAA57443)"
FT /evidence="ECO:0000305"
FT CONFLICT 226
FT /note="W -> Y (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 270
FT /note="Missing (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 289
FT /note="I -> L (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 310
FT /note="Y -> SH (in Ref. 2)"
FT /evidence="ECO:0000305"
FT STRAND 40..45
FT /evidence="ECO:0007829|PDB:3ZIW"
FT HELIX 54..56
FT /evidence="ECO:0007829|PDB:3ZIW"
FT STRAND 57..63
FT /evidence="ECO:0007829|PDB:3ZIW"
FT STRAND 68..78
FT /evidence="ECO:0007829|PDB:3ZIW"
FT STRAND 80..86
FT /evidence="ECO:0007829|PDB:3ZIW"
FT HELIX 93..104
FT /evidence="ECO:0007829|PDB:3ZIW"
FT STRAND 110..121
FT /evidence="ECO:0007829|PDB:3ZIW"
FT STRAND 127..145
FT /evidence="ECO:0007829|PDB:3ZIW"
FT STRAND 148..170
FT /evidence="ECO:0007829|PDB:3ZIW"
FT HELIX 171..173
FT /evidence="ECO:0007829|PDB:3ZIW"
FT HELIX 176..179
FT /evidence="ECO:0007829|PDB:3ZIW"
FT STRAND 188..190
FT /evidence="ECO:0007829|PDB:3AM2"
FT STRAND 195..199
FT /evidence="ECO:0007829|PDB:3ZIW"
FT STRAND 206..210
FT /evidence="ECO:0007829|PDB:2QUO"
FT HELIX 211..216
FT /evidence="ECO:0007829|PDB:2QUO"
FT TURN 219..221
FT /evidence="ECO:0007829|PDB:2QUO"
FT STRAND 223..227
FT /evidence="ECO:0007829|PDB:2QUO"
FT STRAND 238..244
FT /evidence="ECO:0007829|PDB:2QUO"
FT STRAND 246..255
FT /evidence="ECO:0007829|PDB:2QUO"
FT STRAND 259..265
FT /evidence="ECO:0007829|PDB:2QUO"
FT HELIX 267..269
FT /evidence="ECO:0007829|PDB:3ZIW"
FT STRAND 272..277
FT /evidence="ECO:0007829|PDB:2QUO"
FT STRAND 281..283
FT /evidence="ECO:0007829|PDB:2QUO"
FT STRAND 285..291
FT /evidence="ECO:0007829|PDB:2QUO"
FT STRAND 293..304
FT /evidence="ECO:0007829|PDB:2QUO"
FT STRAND 307..310
FT /evidence="ECO:0007829|PDB:6OV3"
FT STRAND 313..318
FT /evidence="ECO:0007829|PDB:2QUO"
SQ SEQUENCE 319 AA; 35330 MW; 2BFB99A7B0245E6F CRC64;
MLSNNLNPMV FENAKEVFLI SEDLKTPINI TNSNSNLSDG LYVIDKGDGW ILGEPSVVSS
QILNPNETGT FSQSLTKSKE VSINVNFSVG FTSEFIQASV EYGFGITIGE QNTIERSVST
TAGPNEYVYY KVYATYRKYQ AIRISHGNIS DDGSIYKLTG IWLSKTSADS LGNIDQGSLI
ETGERCVLTV PSTDIEKEIL DLAAATERLN LTDALNSNPA GNLYDWRSSN SYPWTQKLNL
HLTITATGQK YRILASKIVD FNIYSNNFNN LVKLEQSLGD GVKDHYVDIS LDAGQYVLVM
KANSSYSGNY PYSILFQKF
