ID   ELTP_MYCS2              Reviewed;         477 AA.
AC   A0QXD9;
DT   17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Erythritol/L-threitol-binding protein {ECO:0000303|PubMed:26560079};
DE   Flags: Precursor;
GN   Name=eltP {ECO:0000303|PubMed:26560079};
GN   OrderedLocusNames=MSMEG_3266 {ECO:0000312|EMBL:ABK76127.1},
GN   MSMEI_3182 {ECO:0000312|EMBL:AFP39645.1};
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=26560079; DOI=10.1021/jacs.5b08968;
RA   Huang H., Carter M.S., Vetting M.W., Al-Obaidi N., Patskovsky Y.,
RA   Almo S.C., Gerlt J.A.;
RT   "A general strategy for the discovery of metabolic pathways: D-threitol, L-
RT   threitol, and erythritol utilization in Mycobacterium smegmatis.";
RL   J. Am. Chem. Soc. 137:14570-14573(2015).
CC   -!- FUNCTION: Part of an ABC transporter complex involved in erythritol/L-
CC       threitol import. Binds erythritol and L-threitol. Functions in the
CC       transport for the degradation pathways of erythritol and L-threitol,
CC       that allow M.smegmatis to grow on these compounds as the sole carbon
CC       source. {ECO:0000269|PubMed:26560079}.
CC   -!- INDUCTION: Up-regulated during growth on erythritol, D-threitol or L-
CC       threitol relative to growth on glycerol. {ECO:0000269|PubMed:26560079}.
CC   -!- PTM: Predicted to be exported by the Tat system. The position of the
CC       signal peptide cleavage has not been experimentally proven.
CC       {ECO:0000255|PROSITE-ProRule:PRU00648}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene are defective for growth
CC       on erythritol or on L-threitol. {ECO:0000269|PubMed:26560079}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein 1 family.
CC       {ECO:0000305}.
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DR   EMBL; CP000480; ABK76127.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP39645.1; -; Genomic_DNA.
DR   RefSeq; YP_887577.1; NC_008596.1.
DR   AlphaFoldDB; A0QXD9; -.
DR   SMR; A0QXD9; -.
DR   STRING; 246196.MSMEI_3182; -.
DR   PRIDE; A0QXD9; -.
DR   EnsemblBacteria; ABK76127; ABK76127; MSMEG_3266.
DR   EnsemblBacteria; AFP39645; AFP39645; MSMEI_3182.
DR   KEGG; msg:MSMEI_3182; -.
DR   KEGG; msm:MSMEG_3266; -.
DR   PATRIC; fig|246196.19.peg.3227; -.
DR   eggNOG; COG1653; Bacteria.
DR   OMA; WSLAMNA; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0030246; F:carbohydrate binding; IDA:UniProtKB.
DR   GO; GO:0008643; P:carbohydrate transport; IGC:UniProtKB.
DR   GO; GO:0009758; P:carbohydrate utilization; IMP:UniProtKB.
DR   GO; GO:0071322; P:cellular response to carbohydrate stimulus; IDA:UniProtKB.
DR   InterPro; IPR006059; SBP.
DR   InterPro; IPR006311; TAT_signal.
DR   Pfam; PF01547; SBP_bac_1; 1.
DR   PROSITE; PS51318; TAT; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; Signal; Sugar transport; Transport.
FT   SIGNAL          1..38
FT                   /note="Tat-type signal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00648"
FT   CHAIN           39..477
FT                   /note="Erythritol/L-threitol-binding protein"
FT                   /id="PRO_5005658573"
SQ   SEQUENCE   477 AA;  52812 MW;  5030A7B3F502BCFC CRC64;
     MMSRESQPGL HRQLSRRNML AAMGLAGAAA VSLPVLSACG VGGRTNAPNG ASEVTGGFDW
     RKASGSTINI LQTPHPYQQS YQPLLKEFTE LTGINVNVDL VPEADYFTKL NTELAGGTGK
     HDAFMLGAYF IWQYGPPGWI EDLNPWLQNS SATNAEYDFE DIFEGLRTST RWDFELGNPL
     GTGGQWAIPW GFENNVVAYN KAYFDQRGIT KLPDNFDDFI QLAIDLTDRS ENRYGIATRG
     SKSWATIHPG FMTQYVREGA VDYTFDGTDL VAEMDSDKAV EFTRKWIEMQ HKAGPTSWTT
     YDYPNATGDL GDGTAMMVYD ADSATYPKNK PGASAQAGNL GWYPGPAGPD GNYKTNLWTW
     TWAMNANSRN KLPAWLFIQW ATGKESMNKA VEGGIYADPV RQSVFDTTFK RIAADQHGYL
     ETFETVIGSS KIQFTPQKKF FDTTKDWAVA LQDIYGGDDA ASRLRSLAKT NTSKVNL