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ELYA_ALKHC
ID   ELYA_ALKHC              Reviewed;         361 AA.
AC   P41363; Q53294; Q9KEJ7;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   03-AUG-2022, entry version 140.
DE   RecName: Full=Thermostable alkaline protease;
DE            EC=3.4.21.-;
DE   Flags: Precursor;
GN   OrderedLocusNames=BH0855;
OS   Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344
OS   / JCM 9153 / C-125) (Bacillus halodurans).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus.
OX   NCBI_TaxID=272558;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AH-101 / JCM 9161 / FERM 10531;
RX   PubMed=1369007; DOI=10.1007/bf00169427;
RA   Takami H., Kobayashi T., Aono R., Horikoshi K.;
RT   "Molecular cloning, nucleotide sequence and expression of the structural
RT   gene for a thermostable alkaline protease from Bacillus sp. no. AH-101.";
RL   Appl. Microbiol. Biotechnol. 38:101-108(1992).
RN   [2]
RP   SEQUENCE REVISION.
RA   Takami H., Kobayashi T., Aono R., Horikoshi K.;
RL   Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125;
RX   PubMed=11058132; DOI=10.1093/nar/28.21.4317;
RA   Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F.,
RA   Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.;
RT   "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans
RT   and genomic sequence comparison with Bacillus subtilis.";
RL   Nucleic Acids Res. 28:4317-4331(2000).
RN   [4]
RP   PROTEIN SEQUENCE OF 94-113.
RC   STRAIN=AH-101 / JCM 9161 / FERM 10531;
RX   PubMed=1370008; DOI=10.1007/bf00172544;
RA   Takami H., Akiba T., Horikoshi K.;
RT   "Characterization of an alkaline protease from Bacillus sp. no. AH-101.";
RL   Appl. Microbiol. Biotechnol. 33:519-523(1990).
CC   -!- FUNCTION: Shows keratinolytic activity.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC       Note=Binds 2 calcium ions per subunit. {ECO:0000250};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 12-13.;
CC       Temperature dependence:
CC         Thermostable.;
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR   EMBL; D13158; BAA02443.2; -; Genomic_DNA.
DR   EMBL; BA000004; BAB04574.1; -; Genomic_DNA.
DR   PIR; G83756; G83756.
DR   RefSeq; WP_010897028.1; NC_002570.2.
DR   AlphaFoldDB; P41363; -.
DR   SMR; P41363; -.
DR   MEROPS; S08.046; -.
DR   PRIDE; P41363; -.
DR   EnsemblBacteria; BAB04574; BAB04574; BAB04574.
DR   KEGG; bha:BH0855; -.
DR   eggNOG; COG1404; Bacteria.
DR   HOGENOM; CLU_011263_15_7_9; -.
DR   OMA; SGQYSWI; -.
DR   OrthoDB; 923655at2; -.
DR   Proteomes; UP000001258; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd07477; Peptidases_S8_Subtilisin_subset; 1.
DR   Gene3D; 3.30.70.80; -; 1.
DR   Gene3D; 3.40.50.200; -; 1.
DR   InterPro; IPR000209; Peptidase_S8/S53_dom.
DR   InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR   InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR   InterPro; IPR022398; Peptidase_S8_His-AS.
DR   InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR   InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR   InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR   InterPro; IPR034202; Subtilisin_Carlsberg-like.
DR   Pfam; PF00082; Peptidase_S8; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   SUPFAM; SSF52743; SSF52743; 1.
DR   PROSITE; PS51892; SUBTILASE; 1.
DR   PROSITE; PS00136; SUBTILASE_ASP; 1.
DR   PROSITE; PS00137; SUBTILASE_HIS; 1.
DR   PROSITE; PS00138; SUBTILASE_SER; 1.
PE   1: Evidence at protein level;
KW   Calcium; Direct protein sequencing; Hydrolase; Metal-binding; Protease;
KW   Reference proteome; Secreted; Serine protease; Signal; Zymogen.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   PROPEP          25..93
FT                   /evidence="ECO:0000269|PubMed:1370008"
FT                   /id="PRO_0000027012"
FT   CHAIN           94..361
FT                   /note="Thermostable alkaline protease"
FT                   /id="PRO_0000027013"
FT   DOMAIN          97..360
FT                   /note="Peptidase S8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        124
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        154
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        307
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   BINDING         94
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         132
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         165
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         167
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         169
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         171
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         255
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         257
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         260
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   VARIANT         38
FT                   /note="E -> D (in strain: AH-101)"
FT   VARIANT         69..70
FT                   /note="KK -> EE (in strain: AH-101)"
FT   VARIANT         74..77
FT                   /note="KLKK -> ELQN (in strain: AH-101)"
FT   VARIANT         86
FT                   /note="K -> E (in strain: AH-101)"
FT   VARIANT         104
FT                   /note="N -> S (in strain: AH-101)"
FT   VARIANT         292
FT                   /note="N -> Y (in strain: AH-101)"
FT   VARIANT         346..347
FT                   /note="PS -> SN (in strain: AH-101)"
SQ   SEQUENCE   361 AA;  38116 MW;  24BF004F9E3E8474 CRC64;
     MRQSLKVMVL STVALLFMAN PAAASEEKKE YLIVVEPEEV SAQSVEESYD VDVIHEFEEI
     PVIHAELTKK ELKKLKKDPN VKAIEKNAEV TISQTVPWGI SFINTQQAHN RGIFGNGARV
     AVLDTGIASH PDLRIAGGAS FISSEPSYHD NNGHGTHVAG TIAALNNSIG VLGVAPSADL
     YAVKVLDRNG SGSLASVAQG IEWAINNNMH IINMSLGSTS GSSTLELAVN RANNAGILLV
     GAAGNTGRQG VNYPARYSGV MAVAAVDQNG QRASFSTYGP EIEISAPGVN VNSTYTGNRY
     VSLSGTSMAT PHVAGVAALV KSRYPSYTNN QIRQRINQTA TYLGSPSLYG NGLVHAGRAT
     Q
 
 
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