ELYA_BACYA
ID ELYA_BACYA Reviewed; 378 AA.
AC P20724;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Alkaline elastase YaB;
DE EC=3.4.21.-;
DE Flags: Precursor;
GN Name=ale;
OS Bacillus sp. (strain YaB).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=72578;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2670913; DOI=10.1128/jb.171.9.5232-5236.1989;
RA Kaneko R., Koyama N., Tsai Y.-C., Juang R.-Y., Yoda K., Yamasaki M.;
RT "Molecular cloning of the structural gene for alkaline elastase YaB, a new
RT subtilisin produced by an alkalophilic Bacillus strain.";
RL J. Bacteriol. 171:5232-5236(1989).
RN [2]
RP PROTEIN SEQUENCE OF 111-164.
RA Tsai Y.-C., Lin Y.-T., Li Y.-F., Yamasaki M., Tamura G.;
RT "Characterization of an alkaline elastase from alkalophilic Bacillus Ya-
RT B.";
RL Biochim. Biophys. Acta 883:439-447(1986).
CC -!- FUNCTION: Digests elastin efficiently, has a substrate preference for
CC Ala in P1 position.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC Note=Binds 2 calcium ions per subunit. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR EMBL; M28537; AAA87324.1; -; Genomic_DNA.
DR AlphaFoldDB; P20724; -.
DR SMR; P20724; -.
DR MEROPS; S08.157; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd07477; Peptidases_S8_Subtilisin_subset; 1.
DR Gene3D; 3.30.70.80; -; 1.
DR Gene3D; 3.40.50.200; -; 1.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR InterPro; IPR022398; Peptidase_S8_His-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR InterPro; IPR034202; Subtilisin_Carlsberg-like.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; SSF52743; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00136; SUBTILASE_ASP; 1.
DR PROSITE; PS00137; SUBTILASE_HIS; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 1: Evidence at protein level;
KW Calcium; Direct protein sequencing; Hydrolase; Metal-binding; Protease;
KW Secreted; Serine protease; Signal; Zymogen.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT PROPEP 28..110
FT /evidence="ECO:0000269|Ref.2"
FT /id="PRO_0000027014"
FT CHAIN 111..378
FT /note="Alkaline elastase YaB"
FT /id="PRO_0000027015"
FT DOMAIN 114..377
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 141
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 171
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 324
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT BINDING 111
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 149
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 182
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 184
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 186
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 188
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 272
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 274
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 277
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
SQ SEQUENCE 378 AA; 38793 MW; 5A8FD8CC0C62687D CRC64;
MNKKMGKIVA GTALIISVAF SSSIAQAAEE AKEKYLIGFK EQEVMSQFVD QIDGDEYSIS
SQAEDVEIDL LHEFDFIPVL SVELDPEDVD ALELDPAIAY IEEDAEVTTM QTVPWGINRV
QAPIAQSRGF TGTGVRVAVL DTGISNHADL RIRGGASFVP GEPNISDGNG HGTQVAGTIA
ALNNSIGVLG VAPNVDLYGV KVLGASGSGS ISGIAQGLQW AANNGMHIAN MSLGSSAGSA
TMEQAVNQAT ASGVLVVAAS GNSGAGNVGF PARYANAMAV GATDQNNNRA TFSQYGAGLD
IVAPGVGVQS TVPGNGYASF NGTSMATPHV AGVAALVKQK NPSWSNVQIR NHLKNTATNL
GNTTQFGSGL VNAEAATR
