ELYS_HALCR
ID ELYS_HALCR Reviewed; 156 AA.
AC Q01380;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 25-MAY-2022, entry version 65.
DE RecName: Full=Egg-lysin;
DE AltName: Full=Sperm-lysin;
DE Flags: Precursor;
OS Haliotis cracherodii (Black abalone).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Vetigastropoda; Lepetellida; Haliotoidea; Haliotidae; Haliotis.
OX NCBI_TaxID=6455;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Lee Y.H., Vacquier V.D.;
RT "The divergence of species-specific abalone sperm lysins is promoted by
RT positive Darwinian selection.";
RL Biol. Bull. 182:97-104(1992).
CC -!- FUNCTION: Creates a 3 um hole in the egg vitelline layer through which
CC the sperm passes. Does not have enzyme activity. Species-specific
CC interaction between the sperm protein lysin and the egg protein VERL
CC exposes a basic surface on lysin that may dissociate the egg vitelline
CC layer via electrostatic repulsion. Plays a role in ensuring species-
CC specific fertilization. {ECO:0000250|UniProtKB:P04552}.
CC -!- SUBUNIT: Monomer. Homodimer. Molecules associate into dimers and then
CC rapidly dissociate again. Interacts (as a monomer) with the egg
CC vitelline layer protein VERL (via VERL repeats); each VERL chain can
CC bind multiple copies of lysin. {ECO:0000250|UniProtKB:P04552}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC lumen {ECO:0000250|UniProtKB:P04552}.
CC -!- TISSUE SPECIFICITY: Sperm. {ECO:0000305}.
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DR EMBL; M59971; AAA29201.1; -; mRNA.
DR AlphaFoldDB; Q01380; -.
DR SMR; Q01380; -.
DR GO; GO:0043160; C:acrosomal lumen; ISS:UniProtKB.
DR GO; GO:0007338; P:single fertilization; ISS:UniProtKB.
DR CDD; cd00243; Lysin-Sp18; 1.
DR Gene3D; 1.20.150.10; -; 1.
DR InterPro; IPR001379; Egg_lysin.
DR InterPro; IPR035916; Fertil_protein_sf.
DR Pfam; PF01303; Egg_lysin; 1.
DR PRINTS; PR01882; LYSIN.
DR SUPFAM; SSF47082; SSF47082; 1.
PE 2: Evidence at transcript level;
KW Cytoplasmic vesicle; Fertilization; Signal.
FT SIGNAL 1..18
FT CHAIN 19..156
FT /note="Egg-lysin"
FT /id="PRO_0000021167"
SQ SEQUENCE 156 AA; 18657 MW; 6E9EF98E72D0CA32 CRC64;
MKLLVLCVFA MMATLAVSRR YQFVQHQYIR KAFEVALKVE IIAGFDRTLV KWLRNHGRGL
NENQRKVLYF VNRRYMQTHW QNYMLWIVKK TNALGRPPVV ADYRALGAEI GRRIDMGYFY
NFLKNRVMIP KYLPYMRRLN NMRPEDVPVA NRNPGK