EM514_ARATH
ID EM514_ARATH Reviewed; 202 AA.
AC Q8L557; Q9FJJ7; Q9FJJ8;
DT 10-MAY-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Protein EMBRYO DEFECTIVE 514 {ECO:0000303|PubMed:15341625};
DE AltName: Full=Protein DOMINO 1 {ECO:0000303|PubMed:15341625};
GN Name=EMB514 {ECO:0000303|PubMed:15341625};
GN Synonyms=DOM1 {ECO:0000303|PubMed:15341625};
GN OrderedLocusNames=At5g62440 {ECO:0000312|Araport:AT5G62440};
GN ORFNames=K19B1.5 {ECO:0000312|EMBL:BAB11494.1},
GN K19B1.6 {ECO:0000312|EMBL:BAB11495.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=15341625; DOI=10.1111/j.1365-313x.2004.02166.x;
RA Lahmy S., Guilleminot J., Cheng C.M., Bechtold N., Albert S., Pelletier G.,
RA Delseny M., Devic M.;
RT "DOMINO1, a member of a small plant-specific gene family, encodes a protein
RT essential for nuclear and nucleolar functions.";
RL Plant J. 39:809-820(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: May play a role in ribosome biogenesis and in determining the
CC rate of cell division. Involved in a process essential for nuclear and
CC nucleolar functions. {ECO:0000269|PubMed:15341625}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15341625}. Note=Does
CC not localize in the nucleolus. {ECO:0000269|PubMed:15341625}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, flowers and embryos at
CC globular stage. {ECO:0000269|PubMed:15341625}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality due to embryo development
CC arrest at globular stage. {ECO:0000269|PubMed:15341625}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB11494.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes.; Evidence={ECO:0000305};
CC Sequence=BAB11495.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes.; Evidence={ECO:0000305};
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DR EMBL; AF371327; AAM21312.1; -; mRNA.
DR EMBL; AB015469; BAB11494.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AB015469; BAB11495.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED97608.1; -; Genomic_DNA.
DR EMBL; BT008568; AAP40395.1; -; mRNA.
DR EMBL; BT008688; AAP40494.1; -; mRNA.
DR EMBL; AK175305; BAD43068.1; -; mRNA.
DR EMBL; AK229718; BAF01556.1; -; mRNA.
DR EMBL; AY087852; AAM65405.1; -; mRNA.
DR RefSeq; NP_201050.2; NM_125638.6.
DR AlphaFoldDB; Q8L557; -.
DR SMR; Q8L557; -.
DR IntAct; Q8L557; 1.
DR STRING; 3702.AT5G62440.1; -.
DR iPTMnet; Q8L557; -.
DR PaxDb; Q8L557; -.
DR PRIDE; Q8L557; -.
DR ProteomicsDB; 222323; -.
DR EnsemblPlants; AT5G62440.1; AT5G62440.1; AT5G62440.
DR GeneID; 836365; -.
DR Gramene; AT5G62440.1; AT5G62440.1; AT5G62440.
DR KEGG; ath:AT5G62440; -.
DR Araport; AT5G62440; -.
DR TAIR; locus:2154089; AT5G62440.
DR eggNOG; ENOG502RXTW; Eukaryota.
DR HOGENOM; CLU_069921_0_0_1; -.
DR InParanoid; Q8L557; -.
DR OMA; HAWPAYL; -.
DR OrthoDB; 1595926at2759; -.
DR PhylomeDB; Q8L557; -.
DR PRO; PR:Q8L557; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8L557; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0017126; P:nucleologenesis; IMP:TAIR.
DR GO; GO:0051302; P:regulation of cell division; IMP:TAIR.
DR InterPro; IPR044673; DCL-like.
DR PANTHER; PTHR33415; PTHR33415; 1.
PE 1: Evidence at protein level;
KW Acetylation; Nucleus; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..202
FT /note="Protein EMBRYO DEFECTIVE 514"
FT /id="PRO_0000439874"
FT REGION 1..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 168..202
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..68
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 202 AA; 22545 MW; 1B7E53403426DCF8 CRC64;
MAEEQEIVDS LSAEVNPDQK VDMEVETATP KAETGDEKRE REETEEEENG GESKKQKVGE
EEKSGPVKLG PKEFVTSVAM FDYFVKFLHF WPTDLDVNKY EHMVLLDLIK KGHSEPEKKI
GGGIKTFQVR THPMWKSRCF FLVREDDTAD DFSFRKCVDQ ILPLPENMKT PGANGNGHGG
GRGGGGGRRG GRGGGRGGRF RR
