EMAL2_MOUSE
ID EMAL2_MOUSE Reviewed; 649 AA.
AC Q7TNG5; E9QK48; Q9DAS8;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Echinoderm microtubule-associated protein-like 2;
DE Short=EMAP-2;
GN Name=Eml2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Placenta;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Tubulin binding protein that inhibits microtubule nucleation
CC and growth, resulting in shorter microtubules.
CC {ECO:0000250|UniProtKB:O95834}.
CC -!- SUBUNIT: Interacts with GRID2 and may also interact with GRID1.
CC Interacts with EML3. Binds unpolymerized tubulins via its WD repeat
CC region. {ECO:0000250|UniProtKB:O95834, ECO:0000250|UniProtKB:Q6P6T4}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000250|UniProtKB:O95834}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:O95834}. Note=Colocalizes with the microtubule
CC cytoskeleton. Colocalizes with the mitotic spindle.
CC {ECO:0000250|UniProtKB:O95834}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q7TNG5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7TNG5-2; Sequence=VSP_024480;
CC Name=3;
CC IsoId=Q7TNG5-3; Sequence=VSP_061498;
CC -!- DOMAIN: Contains a tandem atypical propeller in EMLs (TAPE) domain. The
CC N-terminal beta-propeller is formed by canonical WD repeats; in
CC contrast, the second beta-propeller contains one blade that is formed
CC by discontinuous parts of the polypeptide chain (By similarity).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the WD repeat EMAP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB24122.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK005562; BAB24122.1; ALT_FRAME; mRNA.
DR EMBL; BC055476; AAH55476.1; -; mRNA.
DR CCDS; CCDS52055.1; -. [Q7TNG5-2]
DR CCDS; CCDS85224.1; -. [Q7TNG5-1]
DR RefSeq; NP_001156468.1; NM_001162996.1. [Q7TNG5-1]
DR RefSeq; NP_001334511.1; NM_001347582.1.
DR RefSeq; NP_082429.1; NM_028153.1. [Q7TNG5-2]
DR AlphaFoldDB; Q7TNG5; -.
DR SMR; Q7TNG5; -.
DR BioGRID; 215217; 1.
DR IntAct; Q7TNG5; 1.
DR STRING; 10090.ENSMUSP00000037654; -.
DR iPTMnet; Q7TNG5; -.
DR PhosphoSitePlus; Q7TNG5; -.
DR EPD; Q7TNG5; -.
DR jPOST; Q7TNG5; -.
DR MaxQB; Q7TNG5; -.
DR PaxDb; Q7TNG5; -.
DR PeptideAtlas; Q7TNG5; -.
DR PRIDE; Q7TNG5; -.
DR ProteomicsDB; 277824; -. [Q7TNG5-1]
DR ProteomicsDB; 277825; -. [Q7TNG5-2]
DR ProteomicsDB; 318304; -.
DR Antibodypedia; 2407; 163 antibodies from 25 providers.
DR DNASU; 72205; -.
DR Ensembl; ENSMUST00000048502; ENSMUSP00000037654; ENSMUSG00000040811. [Q7TNG5-2]
DR Ensembl; ENSMUST00000117338; ENSMUSP00000112491; ENSMUSG00000040811. [Q7TNG5-3]
DR Ensembl; ENSMUST00000120595; ENSMUSP00000112447; ENSMUSG00000040811. [Q7TNG5-1]
DR GeneID; 72205; -.
DR KEGG; mmu:72205; -.
DR UCSC; uc009fkz.2; mouse. [Q7TNG5-1]
DR UCSC; uc009fla.2; mouse. [Q7TNG5-2]
DR CTD; 24139; -.
DR MGI; MGI:1919455; Eml2.
DR VEuPathDB; HostDB:ENSMUSG00000040811; -.
DR eggNOG; KOG2106; Eukaryota.
DR GeneTree; ENSGT00940000153887; -.
DR HOGENOM; CLU_011754_0_0_1; -.
DR InParanoid; Q7TNG5; -.
DR OMA; IEDPGRC; -.
DR OrthoDB; 271572at2759; -.
DR PhylomeDB; Q7TNG5; -.
DR TreeFam; TF317832; -.
DR BioGRID-ORCS; 72205; 4 hits in 60 CRISPR screens.
DR ChiTaRS; Eml2; mouse.
DR PRO; PR:Q7TNG5; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q7TNG5; protein.
DR Bgee; ENSMUSG00000040811; Expressed in dentate gyrus of hippocampal formation granule cell and 210 other tissues.
DR ExpressionAtlas; Q7TNG5; baseline and differential.
DR Genevisible; Q7TNG5; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0072686; C:mitotic spindle; ISO:MGI.
DR GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR GO; GO:0008022; F:protein C-terminus binding; ISO:MGI.
DR GO; GO:0043621; F:protein self-association; ISS:UniProtKB.
DR GO; GO:0005102; F:signaling receptor binding; ISO:MGI.
DR GO; GO:0015631; F:tubulin binding; ISS:UniProtKB.
DR GO; GO:0000226; P:microtubule cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0031115; P:negative regulation of microtubule polymerization; ISS:UniProtKB.
DR GO; GO:0010968; P:regulation of microtubule nucleation; ISS:UniProtKB.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR033016; EML2.
DR InterPro; IPR005108; HELP.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR PANTHER; PTHR13720:SF50; PTHR13720:SF50; 1.
DR Pfam; PF03451; HELP; 1.
DR Pfam; PF00400; WD40; 6.
DR SMART; SM00320; WD40; 11.
DR SUPFAM; SSF50998; SSF50998; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton; Microtubule;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..649
FT /note="Echinoderm microtubule-associated protein-like 2"
FT /id="PRO_0000284388"
FT REPEAT 56..93
FT /note="WD 1"
FT REPEAT 97..144
FT /note="WD 2"
FT REPEAT 151..192
FT /note="WD 3"
FT REPEAT 195..234
FT /note="WD 4"
FT REPEAT 241..280
FT /note="WD 5"
FT REPEAT 285..323
FT /note="WD 6"
FT REPEAT 369..406
FT /note="WD 7"
FT REPEAT 410..447
FT /note="WD 8"
FT REPEAT 452..489
FT /note="WD 9"
FT REPEAT 495..535
FT /note="WD 10"
FT REPEAT 564..602
FT /note="WD 11"
FT REPEAT 609..648
FT /note="WD 12"
FT REGION 10..649
FT /note="Tandem atypical propeller in EMLs"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..6
FT /note="MSSFGI -> MATGGRAWGGDRARAGAAGAGGGCGGAMAERGPAFCGLYDTS
FT SLLQYCNDDNLSGTSGMEVDDRVSALEQRLQLQEDELAVLKAALADALRRLRACEEQGA
FT ALRARSTPKGRAPPRLGTTASVCQLLKGLPTRTPLNGSGPPRRVGGYATSPSSPKKEAS
FT SGRSSRRYLSPERLASVRREDPRSRTTSSSSNCSAKKE (in isoform 3)"
FT /id="VSP_061498"
FT VAR_SEQ 61
FT /note="Y -> YPLTSSLSALLEEALGFSSY (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024480"
FT CONFLICT 251
FT /note="K -> R (in Ref. 1; BAB24122)"
FT /evidence="ECO:0000305"
FT COILED Q7TNG5-3:65..106
FT /evidence="ECO:0000255"
SQ SEQUENCE 649 AA; 70734 MW; 7B85AE89FE9ECAAC CRC64;
MSSFGIGKTK EVIFSMEEGS VKMFLRGRPV PMLIPDELAP TYSLDTRSEL PSSRLKLDWV
YGYRGRDCRA NLYLLPTGEV VYFVASVAVL YSVEEQRQRH YLGHNDDIKC LAVHPDMVTI
ATGQVAGTTK EGKPLPPHVR VWDSVSLSTL HVLGLGVFDR AVCCVAFSKS NGGNLLCAVD
ESNDHVLSVW DWAKESKVVD SKCSNEAVLV ATFHPTDPSL LITCGKSHIY FWSLEGGSLS
KRQGLFEKHE KPKYVLCVTF LEGGDVVTGD SGGNLYVWGK GGNRITQEVQ GAHDGGVFAL
CALRDGTLVS GGGRDRRVVL WGSDYSKVQE VEVPEDFGPV RTVAEGRGDT LYVGTTRNSI
LLGSVHTGFS LLVQGHVEEL WGLATHPSRA QFVTCGQDKL VHLWSSETHQ PVWSRSIEDP
ARSAGFHPSG SVLAVGTVTG RWLLLDTETH DLVAIHTDGN EQISVVSFSP DGAYLAVGSH
DNLVYVYTVD QGGRKVSRLG KCSGHSSFIT HLDWAQDSTC FVTNSGDYEI LYWDPVTCKQ
ITSADTVRNV EWATATCVLG FGVFGIWPEG ADGTDINAVA RSHDGKLLVS ADDFGKVHLF
SYPCCQPRAL SHKYGGHSSH VTNVAFLWDD SMALTTGGKD TSVLQWRVA
