位置:首页 > 蛋白库 > EMAL2_MOUSE
EMAL2_MOUSE
ID   EMAL2_MOUSE             Reviewed;         649 AA.
AC   Q7TNG5; E9QK48; Q9DAS8;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 142.
DE   RecName: Full=Echinoderm microtubule-associated protein-like 2;
DE            Short=EMAP-2;
GN   Name=Eml2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=C57BL/6J; TISSUE=Placenta;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=FVB/N; TISSUE=Colon;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC   Spleen, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Tubulin binding protein that inhibits microtubule nucleation
CC       and growth, resulting in shorter microtubules.
CC       {ECO:0000250|UniProtKB:O95834}.
CC   -!- SUBUNIT: Interacts with GRID2 and may also interact with GRID1.
CC       Interacts with EML3. Binds unpolymerized tubulins via its WD repeat
CC       region. {ECO:0000250|UniProtKB:O95834, ECO:0000250|UniProtKB:Q6P6T4}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:O95834}. Cytoplasm, cytoskeleton, spindle
CC       {ECO:0000250|UniProtKB:O95834}. Note=Colocalizes with the microtubule
CC       cytoskeleton. Colocalizes with the mitotic spindle.
CC       {ECO:0000250|UniProtKB:O95834}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q7TNG5-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q7TNG5-2; Sequence=VSP_024480;
CC       Name=3;
CC         IsoId=Q7TNG5-3; Sequence=VSP_061498;
CC   -!- DOMAIN: Contains a tandem atypical propeller in EMLs (TAPE) domain. The
CC       N-terminal beta-propeller is formed by canonical WD repeats; in
CC       contrast, the second beta-propeller contains one blade that is formed
CC       by discontinuous parts of the polypeptide chain (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the WD repeat EMAP family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB24122.1; Type=Frameshift; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AK005562; BAB24122.1; ALT_FRAME; mRNA.
DR   EMBL; BC055476; AAH55476.1; -; mRNA.
DR   CCDS; CCDS52055.1; -. [Q7TNG5-2]
DR   CCDS; CCDS85224.1; -. [Q7TNG5-1]
DR   RefSeq; NP_001156468.1; NM_001162996.1. [Q7TNG5-1]
DR   RefSeq; NP_001334511.1; NM_001347582.1.
DR   RefSeq; NP_082429.1; NM_028153.1. [Q7TNG5-2]
DR   AlphaFoldDB; Q7TNG5; -.
DR   SMR; Q7TNG5; -.
DR   BioGRID; 215217; 1.
DR   IntAct; Q7TNG5; 1.
DR   STRING; 10090.ENSMUSP00000037654; -.
DR   iPTMnet; Q7TNG5; -.
DR   PhosphoSitePlus; Q7TNG5; -.
DR   EPD; Q7TNG5; -.
DR   jPOST; Q7TNG5; -.
DR   MaxQB; Q7TNG5; -.
DR   PaxDb; Q7TNG5; -.
DR   PeptideAtlas; Q7TNG5; -.
DR   PRIDE; Q7TNG5; -.
DR   ProteomicsDB; 277824; -. [Q7TNG5-1]
DR   ProteomicsDB; 277825; -. [Q7TNG5-2]
DR   ProteomicsDB; 318304; -.
DR   Antibodypedia; 2407; 163 antibodies from 25 providers.
DR   DNASU; 72205; -.
DR   Ensembl; ENSMUST00000048502; ENSMUSP00000037654; ENSMUSG00000040811. [Q7TNG5-2]
DR   Ensembl; ENSMUST00000117338; ENSMUSP00000112491; ENSMUSG00000040811. [Q7TNG5-3]
DR   Ensembl; ENSMUST00000120595; ENSMUSP00000112447; ENSMUSG00000040811. [Q7TNG5-1]
DR   GeneID; 72205; -.
DR   KEGG; mmu:72205; -.
DR   UCSC; uc009fkz.2; mouse. [Q7TNG5-1]
DR   UCSC; uc009fla.2; mouse. [Q7TNG5-2]
DR   CTD; 24139; -.
DR   MGI; MGI:1919455; Eml2.
DR   VEuPathDB; HostDB:ENSMUSG00000040811; -.
DR   eggNOG; KOG2106; Eukaryota.
DR   GeneTree; ENSGT00940000153887; -.
DR   HOGENOM; CLU_011754_0_0_1; -.
DR   InParanoid; Q7TNG5; -.
DR   OMA; IEDPGRC; -.
DR   OrthoDB; 271572at2759; -.
DR   PhylomeDB; Q7TNG5; -.
DR   TreeFam; TF317832; -.
DR   BioGRID-ORCS; 72205; 4 hits in 60 CRISPR screens.
DR   ChiTaRS; Eml2; mouse.
DR   PRO; PR:Q7TNG5; -.
DR   Proteomes; UP000000589; Chromosome 7.
DR   RNAct; Q7TNG5; protein.
DR   Bgee; ENSMUSG00000040811; Expressed in dentate gyrus of hippocampal formation granule cell and 210 other tissues.
DR   ExpressionAtlas; Q7TNG5; baseline and differential.
DR   Genevisible; Q7TNG5; MM.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0072686; C:mitotic spindle; ISO:MGI.
DR   GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR   GO; GO:0008022; F:protein C-terminus binding; ISO:MGI.
DR   GO; GO:0043621; F:protein self-association; ISS:UniProtKB.
DR   GO; GO:0005102; F:signaling receptor binding; ISO:MGI.
DR   GO; GO:0015631; F:tubulin binding; ISS:UniProtKB.
DR   GO; GO:0000226; P:microtubule cytoskeleton organization; IBA:GO_Central.
DR   GO; GO:0031115; P:negative regulation of microtubule polymerization; ISS:UniProtKB.
DR   GO; GO:0010968; P:regulation of microtubule nucleation; ISS:UniProtKB.
DR   Gene3D; 2.130.10.10; -; 2.
DR   InterPro; IPR033016; EML2.
DR   InterPro; IPR005108; HELP.
DR   InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   PANTHER; PTHR13720:SF50; PTHR13720:SF50; 1.
DR   Pfam; PF03451; HELP; 1.
DR   Pfam; PF00400; WD40; 6.
DR   SMART; SM00320; WD40; 11.
DR   SUPFAM; SSF50998; SSF50998; 2.
DR   PROSITE; PS50082; WD_REPEATS_2; 4.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton; Microtubule;
KW   Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..649
FT                   /note="Echinoderm microtubule-associated protein-like 2"
FT                   /id="PRO_0000284388"
FT   REPEAT          56..93
FT                   /note="WD 1"
FT   REPEAT          97..144
FT                   /note="WD 2"
FT   REPEAT          151..192
FT                   /note="WD 3"
FT   REPEAT          195..234
FT                   /note="WD 4"
FT   REPEAT          241..280
FT                   /note="WD 5"
FT   REPEAT          285..323
FT                   /note="WD 6"
FT   REPEAT          369..406
FT                   /note="WD 7"
FT   REPEAT          410..447
FT                   /note="WD 8"
FT   REPEAT          452..489
FT                   /note="WD 9"
FT   REPEAT          495..535
FT                   /note="WD 10"
FT   REPEAT          564..602
FT                   /note="WD 11"
FT   REPEAT          609..648
FT                   /note="WD 12"
FT   REGION          10..649
FT                   /note="Tandem atypical propeller in EMLs"
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         1..6
FT                   /note="MSSFGI -> MATGGRAWGGDRARAGAAGAGGGCGGAMAERGPAFCGLYDTS
FT                   SLLQYCNDDNLSGTSGMEVDDRVSALEQRLQLQEDELAVLKAALADALRRLRACEEQGA
FT                   ALRARSTPKGRAPPRLGTTASVCQLLKGLPTRTPLNGSGPPRRVGGYATSPSSPKKEAS
FT                   SGRSSRRYLSPERLASVRREDPRSRTTSSSSNCSAKKE (in isoform 3)"
FT                   /id="VSP_061498"
FT   VAR_SEQ         61
FT                   /note="Y -> YPLTSSLSALLEEALGFSSY (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_024480"
FT   CONFLICT        251
FT                   /note="K -> R (in Ref. 1; BAB24122)"
FT                   /evidence="ECO:0000305"
FT   COILED          Q7TNG5-3:65..106
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   649 AA;  70734 MW;  7B85AE89FE9ECAAC CRC64;
     MSSFGIGKTK EVIFSMEEGS VKMFLRGRPV PMLIPDELAP TYSLDTRSEL PSSRLKLDWV
     YGYRGRDCRA NLYLLPTGEV VYFVASVAVL YSVEEQRQRH YLGHNDDIKC LAVHPDMVTI
     ATGQVAGTTK EGKPLPPHVR VWDSVSLSTL HVLGLGVFDR AVCCVAFSKS NGGNLLCAVD
     ESNDHVLSVW DWAKESKVVD SKCSNEAVLV ATFHPTDPSL LITCGKSHIY FWSLEGGSLS
     KRQGLFEKHE KPKYVLCVTF LEGGDVVTGD SGGNLYVWGK GGNRITQEVQ GAHDGGVFAL
     CALRDGTLVS GGGRDRRVVL WGSDYSKVQE VEVPEDFGPV RTVAEGRGDT LYVGTTRNSI
     LLGSVHTGFS LLVQGHVEEL WGLATHPSRA QFVTCGQDKL VHLWSSETHQ PVWSRSIEDP
     ARSAGFHPSG SVLAVGTVTG RWLLLDTETH DLVAIHTDGN EQISVVSFSP DGAYLAVGSH
     DNLVYVYTVD QGGRKVSRLG KCSGHSSFIT HLDWAQDSTC FVTNSGDYEI LYWDPVTCKQ
     ITSADTVRNV EWATATCVLG FGVFGIWPEG ADGTDINAVA RSHDGKLLVS ADDFGKVHLF
     SYPCCQPRAL SHKYGGHSSH VTNVAFLWDD SMALTTGGKD TSVLQWRVA
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024