EMAL5_RAT
ID EMAL5_RAT Reviewed; 1977 AA.
AC Q6ED65;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 17-APR-2007, sequence version 2.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Echinoderm microtubule-associated protein-like 5;
DE Short=EMAP-5;
DE Short=EMAP-like protein 5;
GN Name=Eml5;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND ALTERNATIVE SPLICING.
RX PubMed=15225882; DOI=10.1016/j.gene.2004.04.012;
RA O'Connor V., Houtman S.H., De Zeeuw C.I., Bliss T.V.P., French P.J.;
RT "Eml5, a novel WD40 domain protein expressed in rat brain.";
RL Gene 336:127-137(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
CC -!- FUNCTION: May modify the assembly dynamics of microtubules, such that
CC microtubules are slightly longer, but more dynamic. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q6ED65-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6ED65-2; Sequence=VSP_024492, VSP_024493;
CC Name=3;
CC IsoId=Q6ED65-3; Sequence=VSP_024490;
CC Name=4;
CC IsoId=Q6ED65-4; Sequence=VSP_024491;
CC -!- TISSUE SPECIFICITY: Highly expressed in brain, especially in
CC hippocampus, cerebellum and olfactory bulb (at protein level).
CC {ECO:0000269|PubMed:15225882}.
CC -!- DEVELOPMENTAL STAGE: Expressed in fore- and hindbrain structures from
CC E13 onwards. {ECO:0000269|PubMed:15225882}.
CC -!- SIMILARITY: Belongs to the WD repeat EMAP family. {ECO:0000305}.
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DR EMBL; AY445136; AAS01608.1; -; mRNA.
DR EMBL; AABR03048720; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03052861; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03050388; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03049430; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; Q6ED65; -.
DR SMR; Q6ED65; -.
DR STRING; 10116.ENSRNOP00000054411; -.
DR CarbonylDB; Q6ED65; -.
DR iPTMnet; Q6ED65; -.
DR PhosphoSitePlus; Q6ED65; -.
DR PaxDb; Q6ED65; -.
DR PRIDE; Q6ED65; -.
DR UCSC; RGD:1303275; rat. [Q6ED65-1]
DR RGD; 1303275; Eml5.
DR eggNOG; KOG2106; Eukaryota.
DR InParanoid; Q6ED65; -.
DR PhylomeDB; Q6ED65; -.
DR PRO; PR:Q6ED65; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 6.
DR InterPro; IPR024977; Apc4_WD40_dom.
DR InterPro; IPR005108; HELP.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF12894; ANAPC4_WD40; 2.
DR Pfam; PF03451; HELP; 3.
DR Pfam; PF00400; WD40; 8.
DR SMART; SM00320; WD40; 28.
DR SUPFAM; SSF50978; SSF50978; 2.
DR SUPFAM; SSF50998; SSF50998; 2.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 6.
DR PROSITE; PS50294; WD_REPEATS_REGION; 7.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Cytoskeleton; Microtubule;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..1977
FT /note="Echinoderm microtubule-associated protein-like 5"
FT /id="PRO_0000284397"
FT REPEAT 59..100
FT /note="WD 1"
FT REPEAT 104..145
FT /note="WD 2"
FT REPEAT 148..187
FT /note="WD 3"
FT REPEAT 195..233
FT /note="WD 4"
FT REPEAT 235..273
FT /note="WD 5"
FT REPEAT 280..321
FT /note="WD 6"
FT REPEAT 323..362
FT /note="WD 7"
FT REPEAT 364..403
FT /note="WD 8"
FT REPEAT 406..445
FT /note="WD 9"
FT REPEAT 449..488
FT /note="WD 10"
FT REPEAT 561..601
FT /note="WD 11"
FT REPEAT 725..766
FT /note="WD 12"
FT REPEAT 770..811
FT /note="WD 13"
FT REPEAT 814..853
FT /note="WD 14"
FT REPEAT 861..900
FT /note="WD 15"
FT REPEAT 901..940
FT /note="WD 16"
FT REPEAT 996..1035
FT /note="WD 17"
FT REPEAT 1038..1077
FT /note="WD 18"
FT REPEAT 1080..1120
FT /note="WD 19"
FT REPEAT 1236..1276
FT /note="WD 20"
FT REPEAT 1420..1471
FT /note="WD 21"
FT REPEAT 1475..1516
FT /note="WD 22"
FT REPEAT 1519..1558
FT /note="WD 23"
FT REPEAT 1568..1606
FT /note="WD 24"
FT REPEAT 1608..1654
FT /note="WD 25"
FT REPEAT 1699..1739
FT /note="WD 26"
FT REPEAT 1741..1782
FT /note="WD 27"
FT REPEAT 1783..1822
FT /note="WD 28"
FT REPEAT 1895..1934
FT /note="WD 29"
FT REPEAT 1940..1977
FT /note="WD 30"
FT REGION 609..629
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1274..1299
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1323..1363
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 615..629
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1167..1977
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_024490"
FT VAR_SEQ 1336..1977
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000305"
FT /id="VSP_024491"
FT VAR_SEQ 1581..1605
FT /note="NNLTFTGTISGDVCVWKDHILCRVV -> VCLLKCCVMCDLECVLPYIHKQH
FT EY (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15225882"
FT /id="VSP_024492"
FT VAR_SEQ 1606..1977
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15225882"
FT /id="VSP_024493"
SQ SEQUENCE 1977 AA; 219808 MW; 39EDE91D646FEF1A CRC64;
MAARSAPSCH LRLEWVYGYR GHQCRNNLYY TAAKEIVYFV AGVGVVYSPR EHRQKFYRGH
SDDIISLALH PERVLVATGQ VGKEPYICVW DSYTVQTVSV LKDVHTHGIA CLAFDLDGQR
LVSVGLDSKN AVCVWDWKRG KMLSMAPGHT DRIFDISWDL YQPNKLVSCG VKHIKFWSLC
GNALTPKRGV FGKTGDLQTI LCLACARDEL TYSGALNGDI YVWKGINLIR TIQGAHTAGI
FSMNACEEGF ATGGRDGCIR LWDLTFKPIT VIDLRETEQG YKGLSVRSVC WRGDHILVGT
QDSEIFEIVV HERNKPFLIM QGHCEGELWA LAVHPTKPLA VTGSDDRSVR IWSLVDHALI
ARCNMDEPIR CAAVNADGVH LALGMKDGSL TVLRVRDMTE VVHIKDRKEA IHELKYSPDG
TYLAVGCNDS SVDIYGVAQR YKKVGECVGS LSFITHLDWS SDSKYLQTND GSGKRLLYKM
PGGKEVTSKE EVKGMHWASW TCVAGLEVNG IWPKYSDIND INSVDGNYVG QVLVTADDYG
VVKLFRYPCL RKGAKFRKYI GHSAHVTNVR WSHDYQWVIS IGGADHSVFQ WKFIPERKLK
DALHIAPQES LTESNSDESD SDLSDVPELD SEIEQETQLT YHRQVYKEDL PQLKEQCKEK
QKSATSKRRE RAPGNSIRLH FIHGYRGYDC RSNLFYTQIG EIVYHVAAVG VIYNRQQNTQ
RFYLGHDDDI LCLAIHPLKD YVATGQVGRD PSIHVWDTET IKPLSILKGY HQYGICAVDF
SADGKRLASV GIDDSHTIVL WDWKKGEKLS VARGSKDKIF VVKMNPYVPD KLITAGIKHM
KFWRRAGGGL IGRKGYVGTL GKNDTMMCAV YGWTEEMAFS GTSTGDVCIW RDVFLVKTVK
AHDGPVFSMH ALEKGFVTGG KDGVVALWDD SFERCLKTYA IKRADLAPGS KGLLLEDNPS
IRAISLGHGH ILVGTKNGEI LEVDKSGPVT LLVQGHMEGE VWGLATHPYL PICATVSDDK
TLRIWDLSPS HCMLAVRKLK KGGRCCCFSP DGKALAVGLN DGSFLMANAD TLEDLVSFHH
RKDVISDIQF SPGSGKYLAV ASHDSFVDIY NVTSSKRVGV CKGATSYITH IDWDSRGKLL
QVNTGAKEQL FFEAPRGKKQ TIPSVEVEKI SWATWTSVLG LCCEGIWPII GEVTDVTASC
LTSDKMVLAT GDDLGFVKLF RYPAKGKFGK FKKYVAHSTH VTNVRWTYDD SMLVTLGGAD
MSLMVWTSEV ERHREKKNCD SEESDTDSEE DGGYDSDVTR ENEISYTIRA LSTNIRPMFG
VKPHLQQKEP SVDERQGVVR GSRPPVSRAP PQPEKLQSNN VGKKKRPIED LVLELAFGYR
GRDCRNNVHY LNDGDDIIYH TASVGILHNV ATGTQSFYQE HNDDILCLTV NQHPKFINIV
ATGQVGDSAD MSATAPSVHI WDAVNKQTLS ILRCSHSKGV CSVSFSATGK LLLSVGLDPE
HTVTIWRWQE GAKIASRGGH NQRIFVAEFR PDSDTQFVSV GIKHVKFWTL AGRALLSKKG
LLSTLEDARM QTMLAVAFGA NNLTFTGTIS GDVCVWKDHI LCRVVARAHN GPVFAMYTTL
RDGLIVTGGK ERPSKEGGAV KLWDQELRRC RAFRLETGQV TDCVRSVCRG KGKILVGTRN
SEIIEVGEKN AACNILVNGH VDGPIWGLAT HPSRDFFLSA AEDGTVRLWD IADKKMLNKV
NLGHAARTVC YSPEGDMVAI GMKNGEFIIL LVSSLKIWGK KRDRRCAIHD IRFSPDSRYL
AVGSSENSVD FYDLTLGPTL NRISYCKDIP SFVIQMDFSA DSRHLQVSSG CYKRHVYEVP
SGKHLVDHAA IDRITWATWT SILGDEVMGI WSRHAEKADV TCACVSHSGI SLVTGDDFGM
VKLYDFPCPE KFAKHKRFLG HSPHVTNIRF TSGDRHVVSA GGDDCSVFVW KCVHTPH
