EMAL_DROME
ID EMAL_DROME Reviewed; 1076 AA.
AC Q9VUI3; A4IJ53; Q8IGY9; Q9NII4; Q9VUI2;
DT 18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 3.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Echinoderm microtubule-associated protein-like CG42247;
GN Name=DCX-EMAP; ORFNames=CG42247;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 931-1076.
RX PubMed=10603347; DOI=10.1242/dev.127.2.291;
RA Lai E.C., Bodner R., Kavaler J., Freschi G., Posakony J.W.;
RT "Antagonism of Notch signaling activity by members of a novel protein
RT family encoded by the Bearded and Enhancer of split gene complexes.";
RL Development 127:291-306(2000).
CC -!- FUNCTION: May modify the assembly dynamics of microtubules, such that
CC microtubules are slightly longer, but more dynamic. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=C;
CC IsoId=Q9VUI3-1; Sequence=Displayed;
CC Name=D;
CC IsoId=Q9VUI3-2; Sequence=VSP_035862;
CC -!- SIMILARITY: Belongs to the WD repeat EMAP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF33205.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE014296; AAF49697.2; -; Genomic_DNA.
DR EMBL; AE014296; AAF49698.2; -; Genomic_DNA.
DR EMBL; BT001524; AAN71279.1; -; mRNA.
DR EMBL; BT030411; ABO52831.1; -; mRNA.
DR EMBL; AF208486; AAF33205.1; ALT_FRAME; Genomic_DNA.
DR RefSeq; NP_648728.2; NM_140471.3. [Q9VUI3-1]
DR RefSeq; NP_648729.1; NM_140472.2. [Q9VUI3-2]
DR AlphaFoldDB; Q9VUI3; -.
DR SMR; Q9VUI3; -.
DR BioGRID; 64942; 12.
DR IntAct; Q9VUI3; 1.
DR STRING; 7227.FBpp0288783; -.
DR PaxDb; Q9VUI3; -.
DR DNASU; 39617; -.
DR EnsemblMetazoa; FBtr0299509; FBpp0288783; FBgn0259099. [Q9VUI3-1]
DR EnsemblMetazoa; FBtr0299510; FBpp0288784; FBgn0259099. [Q9VUI3-2]
DR GeneID; 39617; -.
DR KEGG; dme:Dmel_CG42247; -.
DR UCSC; CG42247-RC; d. melanogaster.
DR CTD; 39617; -.
DR FlyBase; FBgn0259099; DCX-EMAP.
DR VEuPathDB; VectorBase:FBgn0259099; -.
DR eggNOG; KOG2106; Eukaryota.
DR eggNOG; KOG3757; Eukaryota.
DR InParanoid; Q9VUI3; -.
DR PhylomeDB; Q9VUI3; -.
DR Reactome; R-DME-9648025; EML4 and NUDC in mitotic spindle formation.
DR BioGRID-ORCS; 39617; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 39617; -.
DR PRO; PR:Q9VUI3; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0259099; Expressed in antenna and 16 other tissues.
DR ExpressionAtlas; Q9VUI3; baseline and differential.
DR Genevisible; Q9VUI3; DM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0071683; C:sensory dendrite; IDA:FlyBase.
DR GO; GO:0008017; F:microtubule binding; IDA:FlyBase.
DR GO; GO:0008344; P:adult locomotory behavior; IMP:FlyBase.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR GO; GO:0035011; P:melanotic encapsulation of foreign target; IMP:FlyBase.
DR GO; GO:0000226; P:microtubule cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0007605; P:sensory perception of sound; IMP:FlyBase.
DR Gene3D; 2.130.10.10; -; 2.
DR Gene3D; 3.10.20.230; -; 2.
DR InterPro; IPR003533; Doublecortin_dom.
DR InterPro; IPR036572; Doublecortin_dom_sf.
DR InterPro; IPR005108; HELP.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF03607; DCX; 1.
DR Pfam; PF03451; HELP; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00537; DCX; 2.
DR SMART; SM00320; WD40; 9.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF50998; SSF50998; 1.
DR SUPFAM; SSF89837; SSF89837; 2.
DR PROSITE; PS50309; DC; 2.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Cytoskeleton; Microtubule;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..1076
FT /note="Echinoderm microtubule-associated protein-like
FT CG42247"
FT /id="PRO_0000050967"
FT DOMAIN 171..253
FT /note="Doublecortin 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00072"
FT DOMAIN 300..384
FT /note="Doublecortin 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00072"
FT REPEAT 525..571
FT /note="WD 1"
FT REPEAT 576..621
FT /note="WD 2"
FT REPEAT 623..661
FT /note="WD 3"
FT REPEAT 668..706
FT /note="WD 4"
FT REPEAT 712..751
FT /note="WD 5"
FT REPEAT 801..840
FT /note="WD 6"
FT REPEAT 842..878
FT /note="WD 7"
FT REPEAT 881..920
FT /note="WD 8"
FT REPEAT 926..965
FT /note="WD 9"
FT REPEAT 996..1035
FT /note="WD 10"
FT REPEAT 1041..1076
FT /note="WD 11"
FT REGION 1..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..79
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 106..143
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..257
FT /note="Missing (in isoform D)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_035862"
FT CONFLICT 837
FT /note="W -> L (in Ref. 3; AAN71279)"
FT /evidence="ECO:0000305"
FT CONFLICT 865
FT /note="L -> P (in Ref. 3; AAN71279)"
FT /evidence="ECO:0000305"
FT CONFLICT 961
FT /note="S -> V (in Ref. 5; AAF33205)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1076 AA; 119255 MW; 35AA50B66565B6D9 CRC64;
MPQISPNAPL EGNVSPPPTQ PHLDDNLHNG NGNGNGIHSS SSNINKHGSN INGNNDSNRS
SPAKSPKRPV SSSFRIQNTS RYDNDEEDLA ASEQVAGGDR QVPDYWQQRN GATTGATSGR
QSRAISPGYL DNMSENSEQP PVVPLVRSKS RPEISSAAAS RYNNLSYWKA RRVVFYRNGD
PFFPGVELRY RPGRDVTSLD NLLDKISPKM DLPRGARYVF SMDGDRKYHL DELEDGAFYV
VSSFKAFKPA SYGKKNGMWY ASPGNQGWGS KPASRKPSIM EADLGTLSTT SGSIKRSAGR
VIRIINNLDH SVQCRVLLNL RTSQPFEEVL EDLGQVLKIN GAKKMYTGTG QEVRSFSQLR
NEFADVDTFY LATGTALIAG SPIRRSRSRP SVVAAAPILP TDELPKVPLR GARPRSKSRP
RILYAPESEI LRPNGEYTML DIMKEEPTKV TIRGLRRTFY PPVHHAPADN SPPDKKLQLQ
WVHGYRGIDA RRNLWVLPSG ELLYYVAAVA VLFDRDEDAQ RHYTGHTEDI MCMDVHPSRE
LVASGQKAGR DRKSQAHVRI WSTESLQTLY VFGMGELDSG VTAVAFSQLN GGSYILAVDS
GRESILSVWQ WQWGHLLGKV ATLQEGLSGA AFHPLDDNLI ITHGRGHLAF WHRRKDGFFE
RTDIVKQPSR SHVTSVQFEP DGDVITADSD GFITIYSVDS DGAYFVRTEF EAHNKGIGCL
IMLGEGTLLS GGEKDRKIAA WDSLQNYKKI ADTKLPEAAG GVRTIYPQRP GRNDGNIYVG
TTRNNILEGS LQRRFTQVVF GHGRQLWGLA AHPDDELYAT AGHDKHVALW RKNKLIWTIQ
TGYECVALAF HPFGTLAAGS TEGHLLVINC ENGAVMLTLR VCGSPLNCVA YNQVGDMIAM
GSQNGSIYLF RVSRDGFSYK KVNKIRGSQP LTHLDWSMDG NFVQTVTIDF DLLFWDAKSL
SPERSPIAMK DVKWLTNNCT VGFLVAGQWS NRYYSTTNTI VATCSRSAAH DMLASGDAEG
YLRLFRYPCI SPRAEFHESK VYSGMLACVR FLCGDHTLIT VGGTDASLMV WDIVEE
