EMB1_CAEEL
ID EMB1_CAEEL Reviewed; 81 AA.
AC Q09410;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Anaphase-promoting complex subunit emb-1 {ECO:0000305};
DE AltName: Full=Abnormal embryogenesis protein 1 {ECO:0000312|WormBase:K10D2.4};
DE AltName: Full=Anaphase-promoting complex subunit 16 homolog {ECO:0000303|PubMed:21775471};
GN Name=emb-1 {ECO:0000312|WormBase:K10D2.4};
GN Synonyms=apc-16 {ECO:0000312|WormBase:K10D2.4};
GN ORFNames=K10D2.4 {ECO:0000312|WormBase:K10D2.4};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION.
RX PubMed=11134076; DOI=10.1083/jcb.151.7.1469;
RA Golden A., Sadler P.L., Wallenfang M.R., Schumacher J.M., Hamill D.R.,
RA Bates G., Bowerman B., Seydoux G., Shakes D.C.;
RT "Metaphase to anaphase (mat) transition-defective mutants in Caenorhabditis
RT elegans.";
RL J. Cell Biol. 151:1469-1482(2000).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20392738; DOI=10.1242/jcs.061549;
RA Kops G.J., van der Voet M., van der Voet M., Manak M.S., van Osch M.H.,
RA Naini S.M., Brear A., McLeod I.X., Hentschel D.M., Yates J.R.,
RA van den Heuvel S., Shah J.V.;
RT "APC16 is a conserved subunit of the anaphase-promoting
RT complex/cyclosome.";
RL J. Cell Sci. 123:1623-1633(2010).
RN [4]
RP FUNCTION, SUBUNIT, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, AND
RP MUTAGENESIS OF GLU-30.
RX PubMed=21775471; DOI=10.1534/genetics.111.131714;
RA Shakes D.C., Allen A.K., Albert K.M., Golden A.;
RT "emb-1 encodes the APC16 subunit of the Caenorhabditis elegans anaphase-
RT promoting complex.";
RL Genetics 189:549-560(2011).
CC -!- FUNCTION: Probable component of the anaphase promoting
CC complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase
CC that controls progression through mitosis and the G1 phase of the cell
CC cycle (PubMed:20392738). The APC/C complex acts by mediating
CC ubiquitination and subsequent degradation of target proteins (By
CC similarity). Developmental role in early embryogenesis and the
CC metaphase to anaphase transition in meiosis and mitosis
CC (PubMed:11134076, PubMed:21775471). May be required for germline
CC proliferation (PubMed:21775471). Required for male tail development and
CC hermaphrodite vulva formation (PubMed:21775471).
CC {ECO:0000250|UniProtKB:Q96DE5, ECO:0000269|PubMed:11134076,
CC ECO:0000269|PubMed:20392738, ECO:0000269|PubMed:21775471}.
CC -!- PATHWAY: Protein modification; protein ubiquitination. {ECO:0000305}.
CC -!- SUBUNIT: The APC/C is probably composed of at least 12 subunits: apc-2,
CC apc-10, apc-11, cdc-26, emb-1, emb-27, emb-30, mat-1, mat-2, mat-3,
CC such-1 and gfi-3. {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in germ cells.
CC {ECO:0000269|PubMed:21775471}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown is largely embryonic
CC lethal. Fertilized eggs show a metaphase-to-anaphase transition
CC defective (Mat) phenotype. Arrest occurs at meiosis I at the one-cell
CC stage of embryogenesis. {ECO:0000269|PubMed:20392738,
CC ECO:0000269|PubMed:21775471}.
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DR EMBL; FO081618; CCD72859.1; -; Genomic_DNA.
DR PIR; B88451; B88451.
DR RefSeq; NP_498098.1; NM_065697.5.
DR AlphaFoldDB; Q09410; -.
DR BioGRID; 51966; 10.
DR ComplexPortal; CPX-3382; Anaphase Promoting Complex/Cyclosome.
DR IntAct; Q09410; 1.
DR STRING; 6239.K10D2.4; -.
DR EPD; Q09410; -.
DR PaxDb; Q09410; -.
DR EnsemblMetazoa; K10D2.4.1; K10D2.4.1; WBGene00019630.
DR GeneID; 187262; -.
DR KEGG; cel:CELE_K10D2.4; -.
DR UCSC; K10D2.4; c. elegans.
DR CTD; 187262; -.
DR WormBase; K10D2.4; CE02016; WBGene00019630; emb-1.
DR eggNOG; ENOG502TIC9; Eukaryota.
DR HOGENOM; CLU_2560477_0_0_1; -.
DR InParanoid; Q09410; -.
DR OMA; CEHRQAQ; -.
DR OrthoDB; 1594604at2759; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q09410; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00019630; Expressed in embryo and 4 other tissues.
DR GO; GO:0005680; C:anaphase-promoting complex; IC:ComplexPortal.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IC:ComplexPortal.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0051445; P:regulation of meiotic cell cycle; IC:ComplexPortal.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; IC:ComplexPortal.
DR GO; GO:1904666; P:regulation of ubiquitin protein ligase activity; IC:ComplexPortal.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Developmental protein; Meiosis; Mitosis;
KW Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..81
FT /note="Anaphase-promoting complex subunit emb-1"
FT /evidence="ECO:0000305"
FT /id="PRO_0000065406"
FT MUTAGEN 30
FT /note="E->K: In hc57 and hc62; temperature sensitive
FT mutants with no visible phenotype at 16 degrees Celsius,
FT but mutants arrest at metaphase of meiosis I at 24 degrees
FT Celsius."
FT /evidence="ECO:0000269|PubMed:21775471"
SQ SEQUENCE 81 AA; 9310 MW; 381D0EAA0F9C220C CRC64;
MALMYPFHVA QPPLNWSEHL WVSEVSPAKE SFITTICEHR QAQWDNQDLL RHLQDSVAIL
TREDQRHVNA VHAAANMPAN P
