EMB1_DAUCA
ID EMB1_DAUCA Reviewed; 92 AA.
AC P17639; Q5KTS6;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Protein EMB-1 {ECO:0000303|PubMed:2339072};
DE AltName: Full=Carrot ABA-induced in somatic embryos 4 {ECO:0000303|Ref.3};
GN Name=EMB-1 {ECO:0000303|PubMed:2339072};
GN Synonyms=CAISE4 {ECO:0000303|Ref.3}, EMB1 {ECO:0000312|EMBL:BAD86647.1};
OS Daucus carota (Wild carrot).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC Daucus; Daucus sect. Daucus.
OX NCBI_TaxID=4039;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Danvers Half-long; TISSUE=Embryo;
RX PubMed=2339072; DOI=10.1093/nar/18.9.2826;
RA Ulrich T.H., Wurtele E.S., Nikolau B.J.;
RT "Sequence of EMB-1, an mRNA accumulating specifically in embryos of
RT carrot.";
RL Nucleic Acids Res. 18:2826-2826(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Danvers Half-long; TISSUE=Embryo;
RX PubMed=8108498; DOI=10.1104/pp.102.1.303;
RA Wurtele E.S., Wang H., Durgerian S., Nikolau B.J., Ulrich T.H.;
RT "Characterization of a gene that is expressed early in somatic
RT embryogenesis of Daucus carota.";
RL Plant Physiol. 102:303-312(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION BY ABSCISIC ACID.
RC STRAIN=cv. US-Harumakigosun; TISSUE=Somatic embryo;
RA Shiota H., Yang G., Shen S., Eun C.H., Watabe K., Tanaka I., Kamada H.;
RT "Isolation and characterization of six abscisic acid-inducible genes from
RT carrot somatic embryos.";
RL Plant Biotechnol. (Sheffield) 21:309-314(2004).
RN [4]
RP STRUCTURE BY NMR, AND SUBCELLULAR LOCATION.
RA Eom J., Baker W.R., Kintanar A., Wurtele E.S.;
RT "The embryo-specific EMB-1 protein of Daucus carota is flexible and
RT unstructured in solution.";
RL Plant Sci. 115:17-24(1996).
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|Ref.4}.
CC -!- TISSUE SPECIFICITY: Expressed in embryogenic cells, somatic embryos and
CC seeds at the later stages of development. In the embryos, expressed in
CC the procambium, the root and shoot meristem and the protoderm of the
CC cotyledons. Not detected in the endosperm or the aleurone layer, in
CC young leaves or roots. {ECO:0000269|PubMed:8108498, ECO:0000269|Ref.3}.
CC -!- DEVELOPMENTAL STAGE: Expressed specifically in embryos, both zygotic
CC and somatic. Present at low levels in globular embryos. At the heart-
CC stage, accumulates throughout the peripheral regions of the embryo and
CC in the mature torpedo embryo expressed in the meristematic regions.
CC {ECO:0000269|PubMed:8108498, ECO:0000303|PubMed:2339072}.
CC -!- INDUCTION: Up-regulated by abscisic acid. {ECO:0000269|Ref.3}.
CC -!- MISCELLANEOUS: Completely disordered protein. Flexible and unstructured
CC in solution. {ECO:0000269|Ref.4}.
CC -!- SIMILARITY: Belongs to the small hydrophilic plant seed protein family.
CC {ECO:0000305}.
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DR EMBL; X17608; CAA35610.1; -; mRNA.
DR EMBL; X60131; CAA42717.1; -; Genomic_DNA.
DR EMBL; AB105042; BAD86647.1; -; mRNA.
DR PIR; JQ2273; JQ2273.
DR AlphaFoldDB; P17639; -.
DR SMR; P17639; -.
DR EnsemblPlants; KZM87718; KZM87718; DCAR_024819.
DR Gramene; KZM87718; KZM87718; DCAR_024819.
DR OMA; IGHEGYQ; -.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0048700; P:acquisition of desiccation tolerance in seed; IEA:EnsemblPlants.
DR DisProt; DP01860; -.
DR InterPro; IPR038956; LEA_5.
DR InterPro; IPR022377; Sm_Hydphi_plant_seed_CS.
DR InterPro; IPR000389; Small_hydrophilic_seed_prot.
DR PANTHER; PTHR34671; PTHR34671; 1.
DR Pfam; PF00477; LEA_5; 1.
DR PROSITE; PS00431; SMALL_HYDR_PLANT_SEED; 1.
PE 1: Evidence at protein level;
KW Nucleus.
FT CHAIN 1..92
FT /note="Protein EMB-1"
FT /id="PRO_0000185678"
FT REGION 1..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 41..56
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 75..92
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 92 AA; 9917 MW; 13E09FA58F5F4FFA CRC64;
MASQQEKKEL DARARQGETV VPGGTGGKSL EAQQHLAEGR SKGGQTRKEQ LGGEGYHEMG
RKGGLSNNDM SGGERAEQEG IDIDESKFRT KK
