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EMB30_CAEEL
ID   EMB30_CAEEL             Reviewed;        1027 AA.
AC   P34441; Q9U4A4;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   23-MAY-2003, sequence version 4.
DT   03-AUG-2022, entry version 152.
DE   RecName: Full=Abnormal embryogenesis protein 30;
GN   Name=emb-30 {ECO:0000312|WormBase:F54C8.3};
GN   ORFNames=F54C8.3 {ECO:0000312|WormBase:F54C8.3};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND MUTAGENESIS OF LEU-46; ALA-223;
RP   GLY-224; GLU-360; GLU-483; LEU-564 AND GLY-701.
RC   STRAIN=Bristol N2;
RX   PubMed=10749938; DOI=10.1091/mbc.11.4.1401;
RA   Furuta T., Tuck S., Kirchner J., Koch B., Auty R., Kitagawa R., Rose A.M.,
RA   Greenstein D.;
RT   "EMB-30: an APC4 homologue required for metaphase-to-anaphase transitions
RT   during meiosis and mitosis in Caenorhabditis elegans.";
RL   Mol. Biol. Cell 11:1401-1419(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=7906398; DOI=10.1038/368032a0;
RA   Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA   Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA   Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA   Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA   Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA   Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA   Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA   Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA   Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA   Wilkinson-Sproat J., Wohldman P.;
RT   "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT   elegans.";
RL   Nature 368:32-38(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [4]
RP   FUNCTION.
RX   PubMed=11134076; DOI=10.1083/jcb.151.7.1469;
RA   Golden A., Sadler P.L., Wallenfang M.R., Schumacher J.M., Hamill D.R.,
RA   Bates G., Bowerman B., Seydoux G., Shakes D.C.;
RT   "Metaphase to anaphase (mat) transition-defective mutants in Caenorhabditis
RT   elegans.";
RL   J. Cell Biol. 151:1469-1482(2000).
RN   [5]
RP   FUNCTION.
RX   PubMed=12498686; DOI=10.1016/s0960-9822(02)01392-1;
RA   Kitagawa R., Law E., Tang L., Rose A.M.;
RT   "The Cdc20 homolog, FZY-1, and its interacting protein, IFY-1, are required
RT   for proper chromosome segregation in Caenorhabditis elegans.";
RL   Curr. Biol. 12:2118-2123(2002).
RN   [6]
RP   FUNCTION.
RX   PubMed=11832245; DOI=10.1016/s1534-5807(02)00114-4;
RA   Rappleye C.A., Tagawa A., Lyczak R., Bowerman B., Aroian R.V.;
RT   "The anaphase-promoting complex and separin are required for embryonic
RT   anterior-posterior axis formation.";
RL   Dev. Cell 2:195-206(2002).
RN   [7]
RP   FUNCTION.
RX   PubMed=15556870; DOI=10.1016/j.cub.2004.11.010;
RA   Juo P., Kaplan J.M.;
RT   "The anaphase-promoting complex regulates the abundance of GLR-1 glutamate
RT   receptors in the ventral nerve cord of C. elegans.";
RL   Curr. Biol. 14:2057-2062(2004).
RN   [8]
RP   FUNCTION, AND MUTAGENESIS OF THR-265 AND MET-480.
RX   PubMed=17237515; DOI=10.1534/genetics.106.067918;
RA   Tarailo M., Kitagawa R., Rose A.M.;
RT   "Suppressors of spindle checkpoint defect (such) mutants identify new mdf-
RT   1/MAD1 interactors in Caenorhabditis elegans.";
RL   Genetics 175:1665-1679(2007).
RN   [9]
RP   FUNCTION.
RX   PubMed=24321454; DOI=10.1016/j.mcn.2013.12.001;
RA   Kowalski J.R., Dube H., Touroutine D., Rush K.M., Goodwin P.R., Carozza M.,
RA   Didier Z., Francis M.M., Juo P.;
RT   "The anaphase-promoting complex (APC) ubiquitin ligase regulates GABA
RT   transmission at the C. elegans neuromuscular junction.";
RL   Mol. Cell. Neurosci. 58:62-75(2014).
CC   -!- FUNCTION: Probable component of the anaphase promoting
CC       complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase
CC       that controls progression through mitosis and the G1 phase of the cell
CC       cycle (By similarity). The APC/C complex acts by mediating
CC       ubiquitination and subsequent degradation of target proteins (By
CC       similarity). Developmental role in early embryogenesis and the
CC       metaphase to anaphase transition in oocyte and spermatocyte meiosis and
CC       mitosis in somatic and germ cells (PubMed:10749938, PubMed:12498686,
CC       PubMed:11134076, PubMed:17237515). Required for embryonic anterior-
CC       posterior axis formation (PubMed:11832245). Negatively regulates ify-1
CC       protein levels during meiosis I (PubMed:12498686). Plays a role in
CC       regulating the abundance of glr-1 receptors in postmitotic neurons,
CC       which may in turn control animal locomotion (PubMed:15556870). Involved
CC       in regulating GABA neurotransmitter release at neuromuscular junctions
CC       in GABA motor neurons (PubMed:24321454). {ECO:0000250|UniProtKB:Q9UJX5,
CC       ECO:0000269|PubMed:10749938, ECO:0000269|PubMed:11134076,
CC       ECO:0000269|PubMed:11832245, ECO:0000269|PubMed:12498686,
CC       ECO:0000269|PubMed:15556870, ECO:0000269|PubMed:17237515,
CC       ECO:0000269|PubMed:24321454}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination. {ECO:0000305}.
CC   -!- SUBUNIT: The APC/C is probably composed of at least 12 subunits: apc-2,
CC       apc-10, apc-11, cdc-26, emb-1, emb-27, emb-30, mat-1, mat-2, mat-3,
CC       such-1 and gfi-3. {ECO:0000305}.
CC   -!- INTERACTION:
CC       P34441; P34766: pal-1; NbExp=2; IntAct=EBI-311903, EBI-311911;
CC   -!- DOMAIN: The C-terminus is specifically required for completion of
CC       oocyte meiotic divisions.
CC   -!- SIMILARITY: Belongs to the APC4 family. {ECO:0000305}.
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DR   EMBL; AF192400; AAF24233.1; -; mRNA.
DR   EMBL; Z22178; CAA80159.3; -; Genomic_DNA.
DR   PIR; G88554; G88554.
DR   PIR; S40745; S40745.
DR   RefSeq; NP_499074.2; NM_066673.6.
DR   AlphaFoldDB; P34441; -.
DR   SMR; P34441; -.
DR   BioGRID; 41519; 17.
DR   ComplexPortal; CPX-3382; Anaphase Promoting Complex/Cyclosome.
DR   IntAct; P34441; 3.
DR   STRING; 6239.F54C8.3; -.
DR   EPD; P34441; -.
DR   PaxDb; P34441; -.
DR   PeptideAtlas; P34441; -.
DR   PRIDE; P34441; -.
DR   EnsemblMetazoa; F54C8.3.1; F54C8.3.1; WBGene00001284.
DR   GeneID; 176322; -.
DR   KEGG; cel:CELE_F54C8.3; -.
DR   UCSC; F54C8.3; c. elegans.
DR   CTD; 176322; -.
DR   WormBase; F54C8.3; CE32676; WBGene00001284; emb-30.
DR   eggNOG; ENOG502SDZW; Eukaryota.
DR   HOGENOM; CLU_004796_0_0_1; -.
DR   InParanoid; P34441; -.
DR   OMA; MMQELGH; -.
DR   OrthoDB; 351579at2759; -.
DR   Reactome; R-CEL-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   SignaLink; P34441; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:P34441; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00001284; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0005680; C:anaphase-promoting complex; IBA:GO_Central.
DR   GO; GO:0034399; C:nuclear periphery; IBA:GO_Central.
DR   GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR   GO; GO:0008595; P:anterior/posterior axis specification, embryo; IMP:WormBase.
DR   GO; GO:0008356; P:asymmetric cell division; IMP:UniProtKB.
DR   GO; GO:1990949; P:metaphase/anaphase transition of meiosis I; IMP:UniProtKB.
DR   GO; GO:0010629; P:negative regulation of gene expression; IMP:UniProtKB.
DR   GO; GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
DR   GO; GO:0009949; P:polarity specification of anterior/posterior axis; IMP:UniProtKB.
DR   GO; GO:0070979; P:protein K11-linked ubiquitination; IBA:GO_Central.
DR   GO; GO:0051445; P:regulation of meiotic cell cycle; IC:ComplexPortal.
DR   GO; GO:0007346; P:regulation of mitotic cell cycle; IC:ComplexPortal.
DR   GO; GO:0030071; P:regulation of mitotic metaphase/anaphase transition; IEA:InterPro.
DR   GO; GO:1904666; P:regulation of ubiquitin protein ligase activity; IC:ComplexPortal.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR024789; APC4.
DR   InterPro; IPR024977; Apc4_WD40_dom.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR13260; PTHR13260; 1.
DR   Pfam; PF12894; ANAPC4_WD40; 1.
DR   SUPFAM; SSF50978; SSF50978; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Developmental protein; Meiosis; Mitosis;
KW   Neurotransmitter transport; Reference proteome; Repeat; Transport;
KW   Ubl conjugation pathway; WD repeat.
FT   CHAIN           1..1027
FT                   /note="Abnormal embryogenesis protein 30"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000050959"
FT   REPEAT          18..65
FT                   /note="WD 1"
FT   REPEAT          70..109
FT                   /note="WD 2"
FT   REGION          619..655
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1005..1027
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        622..649
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         46
FT                   /note="L->W: In TN481."
FT                   /evidence="ECO:0000269|PubMed:10749938"
FT   MUTAGEN         223
FT                   /note="A->V: In TN478."
FT                   /evidence="ECO:0000269|PubMed:10749938"
FT   MUTAGEN         224
FT                   /note="G->D: In TN479."
FT                   /evidence="ECO:0000269|PubMed:10749938"
FT   MUTAGEN         265
FT                   /note="T->I: In h1962ts; temperature sensitive mutant that
FT                   can be maintained at 20 degrees Celsius, but that results
FT                   in the production of unfertilized eggs and therefore
FT                   sterility at 25 degrees Celsius."
FT                   /evidence="ECO:0000269|PubMed:17237515"
FT   MUTAGEN         360
FT                   /note="E->K: In G53."
FT                   /evidence="ECO:0000269|PubMed:10749938"
FT   MUTAGEN         480
FT                   /note="M->I: In h1959ts; temperature sensitive mutant that
FT                   can be maintained at 20 degrees Celsius, but that results
FT                   in embryonic lethality at 25 degrees Celsius."
FT                   /evidence="ECO:0000269|PubMed:17237515"
FT   MUTAGEN         483
FT                   /note="E->K: In AX69."
FT                   /evidence="ECO:0000269|PubMed:10749938"
FT   MUTAGEN         564
FT                   /note="L->P: In TN494."
FT                   /evidence="ECO:0000269|PubMed:10749938"
FT   MUTAGEN         701
FT                   /note="G->R: In TN377."
FT                   /evidence="ECO:0000269|PubMed:10749938"
SQ   SEQUENCE   1027 AA;  117051 MW;  A3D35E7463303888 CRC64;
     MDFSIVKTQI LNNRRTFRTP FKVSSMCFSS QNDLIALGSK TGEILLKRTS WKMIWKTNIN
     MIQAVGTECK LDSSVSALHF SPDGRFLAAA TSKGIIHLLD VETGKVRFSV KAASEKIAKL
     HWNCVREKPF ISNLGEFTTR IKNVEAIEGA IELAETTPNI SQEEIAFVYQ RLDEDGSSFK
     HEDAHKESLE RTLISTETFR ESLQNTILLA TDDMDSKIIV LVAGVFPYME IDISDTLLQY
     NQSLLMLYDM HYSSAFGGVS FLATTYGPFL DCKQNELKPP GAEPKKDGQG CHTLLFNVKL
     NINSSLWDTA LRYIRLLFGF NLYSISLETT KRNWEEQIDN LHSLFDTKTK AVKIGNVLLE
     MLLSGSTDAA GEAFLERGLG TDGLDKIELF ATKHMPEVCR IARGQLSTSA RNLCFQRCEF
     STSLSRYAKF IQLKDDDSFL YDEDPPAYLS ESNIWLNTLE EKINILDMKT RHLGIQCLTM
     MQELGHLVKW ISMTKPFAKT MKVNALMKIK RMNIAKILLY IVRNFIPDPE AVKDIENRLF
     NLKELVSKQK KKDLEDKFDE VEYLYRLERI AEVERKKLIE TFHEKFRFAD LDDDDLTPLL
     FQELDHPIDI FTDSMMEQND SSPFLEEDEG EPEQEEQKPD EEPEPEGEPQ PACDLDRVGS
     FFEKELSQKA LEVLPKCDET FDCVLNERGS RMNSIEIQKM GILRVLEDLA ATLSSPQKIH
     CDKAGNQKKL EVSFIYEITS TPTHQGYQDA KISSVTPSYY KRHPFNQLSG MGRSIQVSVL
     QKNEFSSIVI VPTSDVLPED ENEVNDHVEK LKRCYEFEKI DVTVDKSSAE NRPPGETDAM
     EVDSNELRID VDLGPVVPEY DTLIELSQVH PLHHGELVLL GKFTSAQEGT GVMSQMMRSI
     SSYPHEIPED KDVFAKNSSE DGSSEIPIET LIIHPTIQLA AYLHDDGSKI TIGDMKPEVP
     PIADYEEKTV RKTFRDYQRR RERYREDMGV MNMNDVQYDV LVQEAMDSGR DLTDNGESDE
     DEEDDDI
 
 
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