ID   EMB8_PICGL              Reviewed;         457 AA.
AC   Q40863;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Embryogenesis-associated protein EMB8;
DE            EC=3.1.1.-;
GN   Name=EMB8;
OS   Picea glauca (White spruce) (Pinus glauca).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Picea.
OX   NCBI_TaxID=3330;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RX   PubMed=10350098; DOI=10.1023/a:1006146622614;
RA   Dong J.-Z., Dunstan D.I.;
RT   "Cloning and characterization of six embryogenesis-associated cDNAs from
RT   somatic embryos of Picea glauca and their comparative expression during
RT   zygotic embryogenesis.";
RL   Plant Mol. Biol. 39:859-864(1999).
CC   -!- DEVELOPMENTAL STAGE: In somatic embryos, expressed at low levels during
CC       the first phase of maturation and is most abundant 14 days after
CC       maturation begins. Not detected in zygotic embryos.
CC       {ECO:0000269|PubMed:10350098}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. AB hydrolase 4
CC       family. {ECO:0000305}.
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DR   EMBL; L47118; AAB01571.1; -; Genomic_DNA.
DR   PIR; T09290; T09290.
DR   AlphaFoldDB; Q40863; -.
DR   ESTHER; picgl-emb8; abh_upf0017.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR000952; AB_hydrolase_4_CS.
DR   InterPro; IPR012020; ABHD4.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   PIRSF; PIRSF005211; Ab_hydro_YheT; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   PROSITE; PS01133; UPF0017; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Serine esterase.
FT   CHAIN           1..457
FT                   /note="Embryogenesis-associated protein EMB8"
FT                   /id="PRO_0000212458"
FT   DOMAIN          151..391
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   REGION          39..59
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          438..457
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        231
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        361
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        390
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   457 AA;  51020 MW;  5E6483F80F582A7A CRC64;
     MAISSRSVGM TEIHCNFPQN PFRRISRRIS QFRAGFPDKK PAAGACEEQD ELTSGSAARI
     QRRDKQLPEI IGSDRELMSK LTTLGRPYRH FPFMGNRHVE TIFASFFRSW PVIKSRRECL
     RMEDGGTVEL DWPLEGEDAE LWNGELPVNS PVLILLPGLT GGSDDSYVKH MLLRARKHGW
     HSVVFNSRGC ADSPVTTPQF YSASFTKDLC QVVKHVAVRF SESNIYAVGW SLGANILVRY
     LGEVAGNCPL SGAVSLCNPF NLVIADEDFH KGLGFNNVYD KALARGLRQI FPKHTRLFEG
     IEGEYNIPTV AKARSVRDFD GGLTRVSFGF QSVGDYYSNS SSSLSIKYVQ TSLLCIQASN
     DPIAPSRGIP WEDIKENPNC LLVVTPNGGH LGWVAGDDAP FGAPWTDPLV MEYLEVLEKN
     QIEKPLRRTI DDVHTPRVDS VHTRETNNYK SPIENVN