AGAL_DICDI
ID AGAL_DICDI Reviewed; 385 AA.
AC Q55B10;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Probable alpha-galactosidase;
DE EC=3.2.1.22;
DE AltName: Full=Alpha-D-galactoside galactohydrolase;
DE AltName: Full=Melibiase;
DE Flags: Precursor;
GN Name=melA; ORFNames=DDB_G0271490;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 27 family. {ECO:0000305}.
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DR EMBL; AAFI02000006; EAL71875.1; -; Genomic_DNA.
DR RefSeq; XP_645802.1; XM_640710.1.
DR AlphaFoldDB; Q55B10; -.
DR SMR; Q55B10; -.
DR STRING; 44689.DDB0233870; -.
DR PaxDb; Q55B10; -.
DR EnsemblProtists; EAL71875; EAL71875; DDB_G0271490.
DR GeneID; 8617994; -.
DR KEGG; ddi:DDB_G0271490; -.
DR dictyBase; DDB_G0271490; melA.
DR eggNOG; KOG2366; Eukaryota.
DR HOGENOM; CLU_013093_2_2_1; -.
DR InParanoid; Q55B10; -.
DR OMA; NAFGCDI; -.
DR PhylomeDB; Q55B10; -.
DR Reactome; R-DDI-1660662; Glycosphingolipid metabolism.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR PRO; PR:Q55B10; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0004557; F:alpha-galactosidase activity; IBA:GO_Central.
DR GO; GO:0052692; F:raffinose alpha-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0016139; P:glycoside catabolic process; IBA:GO_Central.
DR GO; GO:0046477; P:glycosylceramide catabolic process; IBA:GO_Central.
DR GO; GO:0009311; P:oligosaccharide metabolic process; IBA:GO_Central.
DR CDD; cd14792; GH27; 1.
DR Gene3D; 2.60.40.1180; -; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR002241; Glyco_hydro_27.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR041233; Melibiase_C.
DR PANTHER; PTHR11452; PTHR11452; 1.
DR Pfam; PF16499; Melibiase_2; 1.
DR Pfam; PF17801; Melibiase_C; 1.
DR PRINTS; PR00740; GLHYDRLASE27.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Disulfide bond; Glycosidase; Hydrolase;
KW Reference proteome; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..385
FT /note="Probable alpha-galactosidase"
FT /id="PRO_0000327851"
FT ACT_SITE 147
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 202
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 180..184
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT DISULFID 40..72
FT /evidence="ECO:0000250"
FT DISULFID 119..149
FT /evidence="ECO:0000250"
SQ SEQUENCE 385 AA; 42312 MW; 873DE98AEEB766BE CRC64;
MMKIAATLLA TIALATVNAL DNGLALTPQM GWSSWNFYAC NINESVIMNT AKAMVSNGMA
DAGYTYVNID DCWAGGRYPN GTVYADPTNF PNGIKYVADY IHSLGLKIGI YTDAGTETCQ
KRVGSYGYEA NDAQTYAEWG IDYVKEDWCY ATLENPQQRY QIMSQALNAT GRPMFFSLCD
WGYENPWTFG MSVGNSWRTT PDIKDNWDSM LSNLMAQAPI TSFSGIGGFN DPDMMMVGNG
GMSNTEYVSH FSLWSLLNAP LIAGCDLIDI DQETLSILTA SEVIAINQDP LGVQGSLVKS
YNGGLQQIWA KPLSNGARAV VLFNTDTNPA TIELLWGNIW MEPSQQLSIR NLWTQTNLGT
FTESYESDSL IPPHGCIMLT LTPTN
