ID   EMBR_MYCBO              Reviewed;         388 AA.
AC   P66800; A0A1R3XXV2; Q11052; X2BHV0;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Transcriptional regulatory protein EmbR;
GN   Name=embR; OrderedLocusNames=BQ2027_MB1298C;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- FUNCTION: Positively regulates the transcription of the embCAB operon.
CC       Exhibits ATPase and GTPase activities (By similarity). {ECO:0000250}.
CC   -!- DOMAIN: Contains a N-terminal winged-helix DNA-binding domain and a
CC       regulatory C-terminal forkhead-associated (FHA) domain, which mediates
CC       binding to a Thr-phosphorylated site in the cognate kinase.
CC       {ECO:0000250}.
CC   -!- PTM: Phosphorylated on threonine residue(s). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the AfsR/DnrI/RedD regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; LT708304; SIT99901.1; -; Genomic_DNA.
DR   RefSeq; NP_854952.1; NC_002945.3.
DR   RefSeq; WP_003406553.1; NC_002945.4.
DR   AlphaFoldDB; P66800; -.
DR   SMR; P66800; -.
DR   EnsemblBacteria; SIT99901; SIT99901; BQ2027_MB1298C.
DR   GeneID; 45425239; -.
DR   PATRIC; fig|233413.5.peg.1423; -.
DR   OMA; QFVDAFA; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd15831; BTAD; 1.
DR   CDD; cd00060; FHA; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR005158; BTAD.
DR   InterPro; IPR000253; FHA_dom.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR008984; SMAD_FHA_dom_sf.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF03704; BTAD; 1.
DR   Pfam; PF00498; FHA; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM01043; BTAD; 1.
DR   SMART; SM00240; FHA; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF48452; SSF48452; 1.
DR   SUPFAM; SSF49879; SSF49879; 1.
DR   PROSITE; PS50006; FHA_DOMAIN; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Phosphoprotein; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..388
FT                   /note="Transcriptional regulatory protein EmbR"
FT                   /id="PRO_0000110009"
FT   DOMAIN          308..357
FT                   /note="FHA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00086"
FT   DNA_BIND        2..105
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
SQ   SEQUENCE   388 AA;  41933 MW;  8221B6EAF1C33354 CRC64;
     MAGSATVEKR LDFGLLGPLQ MTIDGTPVPS GTPKQRAVLA MLVINRNRPV GVDALITALW
     EEWPPSGARA SIHSYVSNLR KLLGGAGIDP RVVLAAAPPG YRLSIPDNTC DLGRFVAEKT
     AGVHAAAAGR FEQASRHLSA ALREWRGPVL DDLRDFQFVE PFATALVEDK VLAHTAKAEA
     EIACGRASAV IAELEALTFE HPYREPLWTQ LITAYYLSDR QSDALGAYRR VKTTLADDLG
     IDPGPTLRAL NERILRQQPL DAKKSAKTTA AGTVTVLDQR TMASGQQAVA YLHDIASGRG
     YPLQAAATRI GRLHDNDIVL DSANVSRHHA VIVDTGTNYV INDLRSSNGV HVQHERIRSA
     VTLNDGDHIR ICDHEFTFQI SAGTHGGT