ID   EMBR_MYCTO              Reviewed;         388 AA.
AC   P9WGJ8; L0T949; P66799; Q11052;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=Transcriptional regulatory protein EmbR;
GN   Name=embR; OrderedLocusNames=MT1305;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Positively regulates the transcription of the embCAB operon.
CC       Exhibits ATPase and GTPase activities (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with RNA polymerase. {ECO:0000250}.
CC   -!- PTM: Phosphorylated on threonine residue(s) by PknH, PknA and PknB.
CC       Phosphorylation enhances the DNA-binding activity of EmbR.
CC       Dephosphorylated by PstP (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the AfsR/DnrI/RedD regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK45564.1; -; Genomic_DNA.
DR   PIR; C70754; C70754.
DR   RefSeq; WP_003406553.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WGJ8; -.
DR   SMR; P9WGJ8; -.
DR   EnsemblBacteria; AAK45564; AAK45564; MT1305.
DR   GeneID; 45425239; -.
DR   KEGG; mtc:MT1305; -.
DR   PATRIC; fig|83331.31.peg.1410; -.
DR   HOGENOM; CLU_004665_0_3_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd15831; BTAD; 1.
DR   CDD; cd00060; FHA; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR005158; BTAD.
DR   InterPro; IPR000253; FHA_dom.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR008984; SMAD_FHA_dom_sf.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF03704; BTAD; 1.
DR   Pfam; PF00498; FHA; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM01043; BTAD; 1.
DR   SMART; SM00240; FHA; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF48452; SSF48452; 1.
DR   SUPFAM; SSF49879; SSF49879; 1.
DR   PROSITE; PS50006; FHA_DOMAIN; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Phosphoprotein; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..388
FT                   /note="Transcriptional regulatory protein EmbR"
FT                   /id="PRO_0000428349"
FT   DOMAIN          308..357
FT                   /note="FHA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00086"
FT   DNA_BIND        2..105
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
SQ   SEQUENCE   388 AA;  41933 MW;  8221B6EAF1C33354 CRC64;
     MAGSATVEKR LDFGLLGPLQ MTIDGTPVPS GTPKQRAVLA MLVINRNRPV GVDALITALW
     EEWPPSGARA SIHSYVSNLR KLLGGAGIDP RVVLAAAPPG YRLSIPDNTC DLGRFVAEKT
     AGVHAAAAGR FEQASRHLSA ALREWRGPVL DDLRDFQFVE PFATALVEDK VLAHTAKAEA
     EIACGRASAV IAELEALTFE HPYREPLWTQ LITAYYLSDR QSDALGAYRR VKTTLADDLG
     IDPGPTLRAL NERILRQQPL DAKKSAKTTA AGTVTVLDQR TMASGQQAVA YLHDIASGRG
     YPLQAAATRI GRLHDNDIVL DSANVSRHHA VIVDTGTNYV INDLRSSNGV HVQHERIRSA
     VTLNDGDHIR ICDHEFTFQI SAGTHGGT