AGAL_ECOLI
ID AGAL_ECOLI Reviewed; 451 AA.
AC P06720; Q2M6I5;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Alpha-galactosidase;
DE EC=3.2.1.22;
DE AltName: Full=Melibiase;
GN Name=melA; Synonyms=mel-7; OrderedLocusNames=b4119, JW4080;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=3031590; DOI=10.1093/nar/15.5.2213;
RA Liljestroem P.L., Liljestroem P.;
RT "Nucleotide sequence of the melA gene, coding for alpha-galactosidase in
RT Escherichia coli K-12.";
RL Nucleic Acids Res. 15:2213-2220(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52.
RX PubMed=6329200; DOI=10.1016/0006-291x(84)90685-5;
RA Shimamoto T., Yazyu H., Futai M., Tsuchiya T.;
RT "Nucleotide sequence of the promoter region of the melibiose operon of
RT Escherichia coli.";
RL Biochem. Biophys. Res. Commun. 121:41-46(1984).
RN [6]
RP PROTEIN SEQUENCE OF 1-20, COFACTOR, AND SUBUNIT.
RX PubMed=2831880; DOI=10.1016/0006-291x(88)90584-0;
RA Nagao Y., Nakada T., Imoto M., Shimamoto T., Sakai S., Tsuda M.,
RA Tsuchiya T.;
RT "Purification and analysis of the structure of alpha-galactosidase from
RT Escherichia coli.";
RL Biochem. Biophys. Res. Commun. 151:236-241(1988).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000269|PubMed:2831880};
CC Note=Binds 1 NAD(+) per subunit. {ECO:0000269|PubMed:2831880};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000269|PubMed:2831880};
CC Note=Binds 1 Mn(2+) ion per subunit. {ECO:0000269|PubMed:2831880};
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:2831880}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA24149.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X04894; CAA28581.1; -; Genomic_DNA.
DR EMBL; K01490; AAA24149.1; ALT_INIT; Genomic_DNA.
DR EMBL; U14003; AAA97019.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77080.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78121.1; -; Genomic_DNA.
DR PIR; A26571; GBECAG.
DR RefSeq; NP_418543.1; NC_000913.3.
DR RefSeq; WP_000986601.1; NZ_SSZK01000018.1.
DR AlphaFoldDB; P06720; -.
DR SMR; P06720; -.
DR BioGRID; 4262685; 8.
DR DIP; DIP-10179N; -.
DR IntAct; P06720; 3.
DR STRING; 511145.b4119; -.
DR ChEMBL; CHEMBL3696; -.
DR CAZy; GH4; Glycoside Hydrolase Family 4.
DR jPOST; P06720; -.
DR PaxDb; P06720; -.
DR PRIDE; P06720; -.
DR EnsemblBacteria; AAC77080; AAC77080; b4119.
DR EnsemblBacteria; BAE78121; BAE78121; BAE78121.
DR GeneID; 948636; -.
DR KEGG; ecj:JW4080; -.
DR KEGG; eco:b4119; -.
DR PATRIC; fig|1411691.4.peg.2581; -.
DR EchoBASE; EB0572; -.
DR eggNOG; COG1486; Bacteria.
DR HOGENOM; CLU_045951_1_1_6; -.
DR InParanoid; P06720; -.
DR OMA; EHIYHAA; -.
DR PhylomeDB; P06720; -.
DR BioCyc; EcoCyc:ALPHAGALACTOSID-MON; -.
DR BioCyc; MetaCyc:ALPHAGALACTOSID-MON; -.
DR PRO; PR:P06720; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0004557; F:alpha-galactosidase activity; IDA:EcoCyc.
DR GO; GO:0030145; F:manganese ion binding; IDA:EcoCyc.
DR GO; GO:0070403; F:NAD+ binding; IDA:EcoCyc.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0052692; F:raffinose alpha-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005995; P:melibiose catabolic process; IMP:EcoCyc.
DR InterPro; IPR019802; GlycHydrolase_4_CS.
DR InterPro; IPR001088; Glyco_hydro_4.
DR InterPro; IPR022616; Glyco_hydro_4_C.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR32092; PTHR32092; 1.
DR Pfam; PF02056; Glyco_hydro_4; 1.
DR Pfam; PF11975; Glyco_hydro_4C; 1.
DR PRINTS; PR00732; GLHYDRLASE4.
DR SUPFAM; SSF51735; SSF51735; 1.
DR SUPFAM; SSF56327; SSF56327; 1.
DR PROSITE; PS01324; GLYCOSYL_HYDROL_F4; 1.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; Direct protein sequencing; Glycosidase; Hydrolase;
KW Manganese; Metal-binding; NAD; Reference proteome.
FT CHAIN 1..451
FT /note="Alpha-galactosidase"
FT /id="PRO_0000169852"
FT ACT_SITE 174
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 5..71
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 151
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 173
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 203
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 287
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 451 AA; 50657 MW; CB20367C0860AB5F CRC64;
MMSAPKITFI GAGSTIFVKN ILGDVFHREA LKTAHIALMD IDPTRLEESH IVVRKLMDSA
GASGKITCHT QQKEALEDAD FVVVAFQIGG YEPCTVTDFE VCKRHGLEQT IADTLGPGGI
MRALRTIPHL WQICEDMTEV CPDATMLNYV NPMAMNTWAM YARYPHIKQV GLCHSVQGTA
EELARDLNID PATLRYRCAG INHMAFYLEL ERKTADGSYV NLYPELLAAY EAGQAPKPNI
HGNTRCQNIV RYEMFKKLGY FVTESSEHFA EYTPWFIKPG REDLIERYKV PLDEYPKRCV
EQLANWHKEL EEYKKASRID IKPSREYAST IMNAIWTGEP SVIYGNVRND GLIDNLPQGC
CVEVACLVDA NGIQPTKVGT LPSHLAALMQ TNINVQTLLT EAILTENRDR VYHAAMMDPH
TAAVLGIDEI YALVDDLIAA HGDWLPGWLH R
