ID   EMC2_DANRE              Reviewed;         297 AA.
AC   Q6TGY8;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=ER membrane protein complex subunit 2 {ECO:0000305};
DE   AltName: Full=Tetratricopeptide repeat protein 35;
DE            Short=TPR repeat protein 35;
GN   Name=emc2; Synonyms=ttc35; ORFNames=zgc:86891;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney marrow;
RX   PubMed=15520368; DOI=10.1073/pnas.0407241101;
RA   Song H.-D., Sun X.-J., Deng M., Zhang G.-W., Zhou Y., Wu X.-Y., Sheng Y.,
RA   Chen Y., Ruan Z., Jiang C.-L., Fan H.-Y., Zon L.I., Kanki J.P., Liu T.X.,
RA   Look A.T., Chen Z.;
RT   "Hematopoietic gene expression profile in zebrafish kidney marrow.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Part of the endoplasmic reticulum membrane protein complex
CC       (EMC) that enables the energy-independent insertion into endoplasmic
CC       reticulum membranes of newly synthesized membrane proteins.
CC       Preferentially accommodates proteins with transmembrane domains that
CC       are weakly hydrophobic or contain destabilizing features such as
CC       charged and aromatic residues. Involved in the cotranslational
CC       insertion of multi-pass membrane proteins in which stop-transfer
CC       membrane-anchor sequences become ER membrane spanning helices. It is
CC       also required for the post-translational insertion of tail-anchored/TA
CC       proteins in endoplasmic reticulum membranes. By mediating the proper
CC       cotranslational insertion of N-terminal transmembrane domains in an N-
CC       exo topology, with translocated N-terminus in the lumen of the ER,
CC       controls the topology of multi-pass membrane proteins. By regulating
CC       the insertion of various proteins in membranes, it is indirectly
CC       involved in many cellular processes. {ECO:0000250|UniProtKB:Q15006}.
CC   -!- SUBUNIT: Component of the ER membrane protein complex (EMC).
CC       {ECO:0000250|UniProtKB:Q15006}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:Q15006}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q15006}; Cytoplasmic side
CC       {ECO:0000250|UniProtKB:Q15006}.
CC   -!- SIMILARITY: Belongs to the EMC2 family. {ECO:0000305}.
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DR   EMBL; AY398368; AAQ97801.1; -; mRNA.
DR   EMBL; BC071517; AAH71517.1; -; mRNA.
DR   RefSeq; NP_998709.1; NM_213544.1.
DR   AlphaFoldDB; Q6TGY8; -.
DR   SMR; Q6TGY8; -.
DR   STRING; 7955.ENSDARP00000006378; -.
DR   PaxDb; Q6TGY8; -.
DR   PRIDE; Q6TGY8; -.
DR   DNASU; 407073; -.
DR   Ensembl; ENSDART00000023238; ENSDARP00000006378; ENSDARG00000003308.
DR   GeneID; 407073; -.
DR   KEGG; dre:407073; -.
DR   CTD; 9694; -.
DR   ZFIN; ZDB-GENE-040625-82; emc2.
DR   eggNOG; KOG3060; Eukaryota.
DR   GeneTree; ENSGT00390000011922; -.
DR   HOGENOM; CLU_052388_1_0_1; -.
DR   InParanoid; Q6TGY8; -.
DR   OMA; YILTCAE; -.
DR   OrthoDB; 956854at2759; -.
DR   PhylomeDB; Q6TGY8; -.
DR   TreeFam; TF312997; -.
DR   PRO; PR:Q6TGY8; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 16.
DR   Bgee; ENSDARG00000003308; Expressed in brain and 30 other tissues.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0072546; C:EMC complex; ISS:UniProtKB.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR   GO; GO:0042406; C:extrinsic component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR   GO; GO:0045050; P:protein insertion into ER membrane by stop-transfer membrane-anchor sequence; ISS:UniProtKB.
DR   GO; GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; ISS:UniProtKB.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR039856; EMC2-like.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR019734; TPR_repeat.
DR   PANTHER; PTHR12760; PTHR12760; 1.
DR   SUPFAM; SSF48452; SSF48452; 1.
DR   PROSITE; PS50005; TPR; 3.
DR   PROSITE; PS50293; TPR_REGION; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Membrane; Reference proteome; Repeat; TPR repeat.
FT   CHAIN           1..297
FT                   /note="ER membrane protein complex subunit 2"
FT                   /id="PRO_0000333732"
FT   REPEAT          87..120
FT                   /note="TPR 1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          155..188
FT                   /note="TPR 2"
FT                   /evidence="ECO:0000255"
FT   REPEAT          192..225
FT                   /note="TPR 3"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   297 AA;  34456 MW;  EC0FA31CA402C74D CRC64;
     MSSISELYDV TWEEMRDKLR KWREENYRNS EQIVDVGEEL INEHASKLGD DIWIIYEQVM
     IAALDCSRDD LAWSCLQELK RQFPDSHRVK RLAGMRLEAL ERYDDANKLY DSILQDDPTN
     TAARKRKICI LRAQGKSSEA IRELNEYLEQ FVGDQEAWHE LSELYINEHD YAKAAFCLEE
     LMMTNPHNHL YCEQYAEVKY TQGGLENLEL ARKYFAQALK LNNRNMRALF GLYMSASHIA
     ASPKVSAKVK KDNVKYAAWA AAQISRAYQM AGRGKKENKC SVKAVEEMLE SLQITMS