EMC2_RAT
ID EMC2_RAT Reviewed; 297 AA.
AC B0BNG0;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=ER membrane protein complex subunit 2 {ECO:0000305};
DE AltName: Full=Tetratricopeptide repeat protein 35 {ECO:0000312|RGD:1310430};
DE Short=TPR repeat protein 35 {ECO:0000312|RGD:1310430};
GN Name=Emc2 {ECO:0000312|RGD:1310430};
GN Synonyms=Ttc35 {ECO:0000312|RGD:1310430};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Part of the endoplasmic reticulum membrane protein complex
CC (EMC) that enables the energy-independent insertion into endoplasmic
CC reticulum membranes of newly synthesized membrane proteins.
CC Preferentially accommodates proteins with transmembrane domains that
CC are weakly hydrophobic or contain destabilizing features such as
CC charged and aromatic residues. Involved in the cotranslational
CC insertion of multi-pass membrane proteins in which stop-transfer
CC membrane-anchor sequences become ER membrane spanning helices. It is
CC also required for the post-translational insertion of tail-anchored/TA
CC proteins in endoplasmic reticulum membranes. By mediating the proper
CC cotranslational insertion of N-terminal transmembrane domains in an N-
CC exo topology, with translocated N-terminus in the lumen of the ER,
CC controls the topology of multi-pass membrane proteins like the G
CC protein-coupled receptors. By regulating the insertion of various
CC proteins in membranes, it is indirectly involved in many cellular
CC processes. {ECO:0000250|UniProtKB:Q15006}.
CC -!- SUBUNIT: Component of the ER membrane protein complex (EMC).
CC {ECO:0000250|UniProtKB:Q15006}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q15006}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:Q15006}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:Q15006}. Note=May also localize to the nuclear
CC envelope. {ECO:0000250|UniProtKB:Q9CRD2}.
CC -!- SIMILARITY: Belongs to the EMC2 family. {ECO:0000305}.
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DR EMBL; BC158805; AAI58806.1; -; mRNA.
DR RefSeq; NP_001107257.1; NM_001113785.1.
DR AlphaFoldDB; B0BNG0; -.
DR SMR; B0BNG0; -.
DR jPOST; B0BNG0; -.
DR PaxDb; B0BNG0; -.
DR PeptideAtlas; B0BNG0; -.
DR PRIDE; B0BNG0; -.
DR Ensembl; ENSRNOT00000115694; ENSRNOP00000088541; ENSRNOG00000005123.
DR GeneID; 362905; -.
DR KEGG; rno:362905; -.
DR UCSC; RGD:1310430; rat.
DR CTD; 9694; -.
DR RGD; 1310430; Emc2.
DR eggNOG; KOG3060; Eukaryota.
DR GeneTree; ENSGT00390000011922; -.
DR InParanoid; B0BNG0; -.
DR OrthoDB; 956854at2759; -.
DR PhylomeDB; B0BNG0; -.
DR TreeFam; TF312997; -.
DR PRO; PR:B0BNG0; -.
DR Proteomes; UP000002494; Chromosome 7.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0072546; C:EMC complex; ISS:UniProtKB.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0042406; C:extrinsic component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0032977; F:membrane insertase activity; IEA:Ensembl.
DR GO; GO:0045050; P:protein insertion into ER membrane by stop-transfer membrane-anchor sequence; ISS:UniProtKB.
DR GO; GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; ISS:UniProtKB.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR039856; EMC2-like.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR PANTHER; PTHR12760; PTHR12760; 1.
DR SMART; SM00028; TPR; 3.
DR SUPFAM; SSF48452; SSF48452; 1.
DR PROSITE; PS50005; TPR; 2.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Endoplasmic reticulum; Membrane; Reference proteome; Repeat;
KW TPR repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q15006"
FT CHAIN 2..297
FT /note="ER membrane protein complex subunit 2"
FT /id="PRO_0000333731"
FT REPEAT 87..120
FT /note="TPR 1"
FT /evidence="ECO:0000255"
FT REPEAT 155..188
FT /note="TPR 2"
FT /evidence="ECO:0000255"
FT REPEAT 192..225
FT /note="TPR 3"
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q15006"
SQ SEQUENCE 297 AA; 34870 MW; 7B8ABD95D4B8A849 CRC64;
MAKVTERYDV TWEEMRDKMR KWREENSRNS EQIMEVGEEL INDYASKLGD DIWIIYEQVM
IAALDYGRDD LALFCLQELR RQFPGSHRVK RLTGMRFEAM ERYDDAIQLY DRILQEDPTN
TAARKRKIAI RKAQGKNVEA IRELNEYLEQ FVGDQEAWHE LAELYINEHD YAKAAFCLEE
LMMTNPHNHL YCQQYAEVKY TQGGLENLEL SRKYFAQALK LNNRNMRALF GLYMSASHIA
SNPKASAKMK KDNIRYAGWA ANQINRAYQF AGRSKKETKS SLKAVEDMLE TLQITQS
