EMC3_DANRE
ID EMC3_DANRE Reviewed; 261 AA.
AC Q7SXW4; Q5IFI3;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=ER membrane protein complex subunit 3;
DE AltName: Full=Protein pob;
DE AltName: Full=Transmembrane protein 111;
GN Name=emc3; Synonyms=pob, tmem111; ORFNames=zgc:63727;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=15716502; DOI=10.1534/genetics.104.036434;
RA Taylor M.R., Kikkawa S., Diez-Juan A., Ramamurthy V., Kawakami K.,
RA Carmeliet P., Brockerhoff S.E.;
RT "The zebrafish pob gene encodes a novel protein required for survival of
RT red cone photoreceptor cells.";
RL Genetics 170:263-273(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney marrow;
RX PubMed=15520368; DOI=10.1073/pnas.0407241101;
RA Song H.-D., Sun X.-J., Deng M., Zhang G.-W., Zhou Y., Wu X.-Y., Sheng Y.,
RA Chen Y., Ruan Z., Jiang C.-L., Fan H.-Y., Zon L.I., Kanki J.P., Liu T.X.,
RA Look A.T., Chen Z.;
RT "Hematopoietic gene expression profile in zebrafish kidney marrow.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AB;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Part of the endoplasmic reticulum membrane protein complex
CC (EMC) that enables the energy-independent insertion into endoplasmic
CC reticulum membranes of newly synthesized membrane proteins.
CC Preferentially accommodates proteins with transmembrane domains that
CC are weakly hydrophobic or contain destabilizing features such as
CC charged and aromatic residues. Involved in the cotranslational
CC insertion of multi-pass membrane proteins in which stop-transfer
CC membrane-anchor sequences become ER membrane spanning helices. It is
CC also required for the post-translational insertion of tail-anchored/TA
CC proteins in endoplasmic reticulum membranes. By mediating the proper
CC cotranslational insertion of N-terminal transmembrane domains in an N-
CC exo topology, with translocated N-terminus in the lumen of the ER,
CC controls the topology of multi-pass membrane proteins like the G
CC protein-coupled receptors. By regulating the insertion of various
CC proteins in membranes, it is indirectly involved in many cellular
CC processes. {ECO:0000250|UniProtKB:Q9P0I2}.
CC -!- SUBUNIT: Component of the ER membrane protein complex (EMC).
CC {ECO:0000250|UniProtKB:Q9P0I2}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q9P0I2}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9P0I2}.
CC -!- TISSUE SPECIFICITY: Localized to the inner segment and synapse in
CC photoreceptor cells. {ECO:0000269|PubMed:15716502}.
CC -!- SIMILARITY: Belongs to the EMC3 family. {ECO:0000305}.
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DR EMBL; AY745978; AAW19648.1; -; mRNA.
DR EMBL; AY398377; AAQ97810.1; -; mRNA.
DR EMBL; BC055222; AAH55222.1; -; mRNA.
DR RefSeq; NP_998670.1; NM_213505.2.
DR AlphaFoldDB; Q7SXW4; -.
DR SMR; Q7SXW4; -.
DR STRING; 7955.ENSDARP00000106382; -.
DR PaxDb; Q7SXW4; -.
DR Ensembl; ENSDART00000129302; ENSDARP00000106382; ENSDARG00000020607.
DR GeneID; 406826; -.
DR KEGG; dre:406826; -.
DR CTD; 55831; -.
DR ZFIN; ZDB-GENE-040426-2904; emc3.
DR eggNOG; KOG3188; Eukaryota.
DR GeneTree; ENSGT00390000005780; -.
DR HOGENOM; CLU_060791_1_0_1; -.
DR InParanoid; Q7SXW4; -.
DR OMA; WYFLTLF; -.
DR OrthoDB; 1482782at2759; -.
DR PhylomeDB; Q7SXW4; -.
DR Reactome; R-DRE-8980692; RHOA GTPase cycle.
DR PRO; PR:Q7SXW4; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 6.
DR Bgee; ENSDARG00000020607; Expressed in camera-type eye and 28 other tissues.
DR GO; GO:0072546; C:EMC complex; ISS:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; IDA:ZFIN.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; ISS:UniProtKB.
DR GO; GO:0030140; C:trans-Golgi network transport vesicle; IDA:ZFIN.
DR GO; GO:0045494; P:photoreceptor cell maintenance; IMP:ZFIN.
DR GO; GO:0045050; P:protein insertion into ER membrane by stop-transfer membrane-anchor sequence; ISS:UniProtKB.
DR GO; GO:0060041; P:retina development in camera-type eye; IMP:ZFIN.
DR GO; GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; ISS:UniProtKB.
DR InterPro; IPR008568; EMC3.
DR InterPro; IPR002809; EMC3/TMCO1.
DR PANTHER; PTHR13116; PTHR13116; 1.
DR Pfam; PF01956; EMC3_TMCO1; 1.
DR PIRSF; PIRSF010045; DUF850_TM_euk; 1.
DR SMART; SM01415; DUF106; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..261
FT /note="ER membrane protein complex subunit 3"
FT /id="PRO_0000211410"
FT TOPO_DOM 1..14
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:Q9P0I2"
FT TRANSMEM 15..38
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q9P0I2"
FT TOPO_DOM 39..114
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9P0I2"
FT TRANSMEM 115..130
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q9P0I2"
FT TOPO_DOM 131..168
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:Q9P0I2"
FT TRANSMEM 169..187
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q9P0I2"
FT TOPO_DOM 188..261
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9P0I2"
FT CONFLICT 25
FT /note="F -> I (in Ref. 1; AAW19648)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 261 AA; 29792 MW; EC717145BD15EEBC CRC64;
MAEPELLLDS NIRLWVVLPI VFITFLVGVI RHYVSILLQS DKKLTLEQVS DSQVLIRSRV
LRENGKYIPK QSFLMRKFYF NNQEDGFFKK TKRKVVPPSP MTDPSMLTDM MKGNVTNVLP
MILIGGWINW TFSGFVTTKV PFPLTLRFKP MLQQGIELLS LDASWVSSAS WYFLNVFGLR
SMYSLILGQD NGADQSRIMQ EQMSGAAMAM PADTNKAFKA EWEALELTDH QWALENVEED
LMSKDLDLSG MFSKDLPTGI F
