EMC5_DANRE
ID EMC5_DANRE Reviewed; 130 AA.
AC Q6TLE4;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=ER membrane protein complex subunit 5 {ECO:0000250|UniProtKB:Q8N4V1};
DE AltName: Full=Membrane magnesium transporter 1 {ECO:0000250|UniProtKB:Q8K273};
GN Name=mmgt1 {ECO:0000250|UniProtKB:Q8K273};
GN Synonyms=emc5 {ECO:0000250|UniProtKB:Q8N4V1}; ORFNames=zgc:92918;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney marrow;
RX PubMed=15520368; DOI=10.1073/pnas.0407241101;
RA Song H.-D., Sun X.-J., Deng M., Zhang G.-W., Zhou Y., Wu X.-Y., Sheng Y.,
RA Chen Y., Ruan Z., Jiang C.-L., Fan H.-Y., Zon L.I., Kanki J.P., Liu T.X.,
RA Look A.T., Chen Z.;
RT "Hematopoietic gene expression profile in zebrafish kidney marrow.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Part of the endoplasmic reticulum membrane protein complex
CC (EMC) that enables the energy-independent insertion into endoplasmic
CC reticulum membranes of newly synthesized membrane proteins.
CC Preferentially accommodates proteins with transmembrane domains that
CC are weakly hydrophobic or contain destabilizing features such as
CC charged and aromatic residues. Involved in the cotranslational
CC insertion of multi-pass membrane proteins in which stop-transfer
CC membrane-anchor sequences become ER membrane spanning helices. It is
CC also required for the post-translational insertion of tail-anchored/TA
CC proteins in endoplasmic reticulum membranes. By mediating the proper
CC cotranslational insertion of N-terminal transmembrane domains in an N-
CC exo topology, with translocated N-terminus in the lumen of the ER,
CC controls the topology of multi-pass membrane proteins like the G
CC protein-coupled receptors (By similarity). By regulating the insertion
CC of various proteins in membranes, it is indirectly involved in many
CC cellular processes. May be involved in Mg(2+) transport (By
CC similarity). {ECO:0000250|UniProtKB:Q8K273,
CC ECO:0000250|UniProtKB:Q8N4V1}.
CC -!- SUBUNIT: Component of the ER membrane protein complex (EMC).
CC {ECO:0000250|UniProtKB:Q8N4V1}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q8N4V1}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q8N4V1}. Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q8K273}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q8N4V1}. Early endosome membrane
CC {ECO:0000250|UniProtKB:Q8K273}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q8N4V1}.
CC -!- SIMILARITY: Belongs to the membrane magnesium transporter (TC 1.A.67)
CC family. {ECO:0000305}.
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DR EMBL; AY394962; AAQ94589.1; -; mRNA.
DR EMBL; BC076369; AAH76369.1; -; mRNA.
DR RefSeq; NP_991162.1; NM_205599.1.
DR AlphaFoldDB; Q6TLE4; -.
DR SMR; Q6TLE4; -.
DR STRING; 7955.ENSDARP00000005525; -.
DR PaxDb; Q6TLE4; -.
DR Ensembl; ENSDART00000016592; ENSDARP00000005525; ENSDARG00000010553.
DR Ensembl; ENSDART00000189931; ENSDARP00000154905; ENSDARG00000109407.
DR GeneID; 402891; -.
DR KEGG; dre:402891; -.
DR CTD; 93380; -.
DR ZFIN; ZDB-GENE-040801-65; mmgt1.
DR eggNOG; KOG3918; Eukaryota.
DR GeneTree; ENSGT00510000047104; -.
DR HOGENOM; CLU_122437_1_0_1; -.
DR InParanoid; Q6TLE4; -.
DR OMA; YYSVQYE; -.
DR OrthoDB; 1558871at2759; -.
DR PhylomeDB; Q6TLE4; -.
DR TreeFam; TF323267; -.
DR PRO; PR:Q6TLE4; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 14.
DR Bgee; ENSDARG00000010553; Expressed in early embryo and 30 other tissues.
DR ExpressionAtlas; Q6TLE4; baseline.
DR GO; GO:0005769; C:early endosome; ISS:UniProtKB.
DR GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0072546; C:EMC complex; ISS:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022890; F:inorganic cation transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0015693; P:magnesium ion transport; ISS:UniProtKB.
DR GO; GO:0045050; P:protein insertion into ER membrane by stop-transfer membrane-anchor sequence; ISS:UniProtKB.
DR GO; GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; ISS:UniProtKB.
DR InterPro; IPR018937; MMgT.
DR Pfam; PF10270; MMgT; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Endosome; Golgi apparatus; Magnesium; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..130
FT /note="ER membrane protein complex subunit 5"
FT /id="PRO_0000286438"
FT TOPO_DOM 1..3
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q8N4V1"
FT TRANSMEM 4..22
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q8N4V1"
FT TOPO_DOM 23..43
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:Q8N4V1"
FT TRANSMEM 44..63
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q8N4V1"
FT TOPO_DOM 64..130
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q8N4V1"
SQ SEQUENCE 130 AA; 14779 MW; 18FDDBD8165722C6 CRC64;
MASSFWKGVV GIGLFALAHA AFSAAQHRSY MRLTEKENET LPIDIVLQTL LSFVITCYGI
VHISGEFKDM DASSELKNKT FDTLRNHPSF YLFNHRGRVL FRTPEQEPST PNAQALPSNP
LRLRKLENFH
