EMC5_DICDI
ID EMC5_DICDI Reviewed; 105 AA.
AC Q86J02; Q555V5;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=ER membrane protein complex subunit 5 homolog {ECO:0000250|UniProtKB:Q8N4V1};
DE AltName: Full=Membrane magnesium transporter homolog {ECO:0000250|UniProtKB:Q8K273};
GN Name=mmgt {ECO:0000250|UniProtKB:Q8K273};
GN Synonyms=emc5 {ECO:0000250|UniProtKB:Q8N4V1}; ORFNames=DDB_G0274571;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Part of the endoplasmic reticulum membrane protein complex
CC (EMC) that enables the energy-independent insertion into endoplasmic
CC reticulum membranes of newly synthesized membrane proteins.
CC Preferentially accommodates proteins with transmembrane domains that
CC are weakly hydrophobic or contain destabilizing features such as
CC charged and aromatic residues. Involved in the cotranslational
CC insertion of multi-pass membrane proteins in which stop-transfer
CC membrane-anchor sequences become ER membrane spanning helices. It is
CC also required for the post-translational insertion of tail-anchored/TA
CC proteins in endoplasmic reticulum membranes. By mediating the proper
CC cotranslational insertion of N-terminal transmembrane domains in an N-
CC exo topology, with translocated N-terminus in the lumen of the ER,
CC controls the topology of multi-pass membrane proteins (By similarity).
CC By regulating the insertion of various proteins in membranes, it is
CC indirectly involved in many cellular processes (By similarity).
CC {ECO:0000250|UniProtKB:Q8K273, ECO:0000250|UniProtKB:Q8N4V1}.
CC -!- SUBUNIT: Component of the ER membrane protein complex (EMC).
CC {ECO:0000250|UniProtKB:Q8N4V1}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q8N4V1}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q8N4V1}.
CC -!- SIMILARITY: Belongs to the membrane magnesium transporter (TC 1.A.67)
CC family. {ECO:0000305}.
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DR EMBL; AAFI02000012; EAL70178.1; -; Genomic_DNA.
DR RefSeq; XP_643955.1; XM_638863.1.
DR AlphaFoldDB; Q86J02; -.
DR SMR; Q86J02; -.
DR STRING; 44689.DDB0233814; -.
DR PaxDb; Q86J02; -.
DR EnsemblProtists; EAL70178; EAL70178; DDB_G0274571.
DR GeneID; 8619383; -.
DR KEGG; ddi:DDB_G0274571; -.
DR dictyBase; DDB_G0274571; mmgt.
DR eggNOG; KOG3918; Eukaryota.
DR HOGENOM; CLU_122437_3_0_1; -.
DR InParanoid; Q86J02; -.
DR OMA; TDQPFEG; -.
DR PhylomeDB; Q86J02; -.
DR PRO; PR:Q86J02; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005769; C:early endosome; ISS:UniProtKB.
DR GO; GO:0072546; C:EMC complex; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022890; F:inorganic cation transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0015693; P:magnesium ion transport; ISS:UniProtKB.
DR GO; GO:0045050; P:protein insertion into ER membrane by stop-transfer membrane-anchor sequence; ISS:UniProtKB.
DR InterPro; IPR018937; MMgT.
DR Pfam; PF10270; MMgT; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Magnesium; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..105
FT /note="ER membrane protein complex subunit 5 homolog"
FT /id="PRO_0000328241"
FT TOPO_DOM 1..3
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q8N4V1"
FT TRANSMEM 4..22
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q8N4V1"
FT TOPO_DOM 23..43
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:Q8N4V1"
FT TRANSMEM 44..63
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q8N4V1"
FT TOPO_DOM 64..105
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q8N4V1"
SQ SEQUENCE 105 AA; 12199 MW; BE43BD55EAA7CDF7 CRC64;
MKKGIAKIFC VIGISIFLHT VYSALQHRKY LRLTDQPFEG VPFDIVVECI ISLFLFAWGI
INSQFLIPIK ASTHLAKKSF DSYDYRPNFT VFNHRGKYLN EILNK
