EMC89_DROME
ID EMC89_DROME Reviewed; 203 AA.
AC Q9W1Y1;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=ER membrane protein complex subunit 8/9 homolog;
GN Name=EMC8-9 {ECO:0000312|FlyBase:FBgn0034791};
GN ORFNames=CG3501 {ECO:0000312|FlyBase:FBgn0034791};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=31263175; DOI=10.1038/s41418-019-0378-6;
RA Xiong L., Zhang L., Yang Y., Li N., Lai W., Wang F., Zhu X., Wang T.;
RT "ER complex proteins are required for rhodopsin biosynthesis and
RT photoreceptor survival in Drosophila and mice.";
RL Cell Death Differ. 27:646-661(2020).
CC -!- FUNCTION: Part of the endoplasmic reticulum membrane protein complex
CC (EMC) that enables the energy-independent insertion into endoplasmic
CC reticulum membranes of newly synthesized multi-pass membrane proteins
CC like rhodopsins. {ECO:0000269|PubMed:31263175}.
CC -!- SUBUNIT: Component of the ER membrane protein complex (EMC).
CC {ECO:0000305|PubMed:31263175}.
CC -!- INTERACTION:
CC Q9W1Y1; Q9VEQ2: EMC2B; NbExp=3; IntAct=EBI-189387, EBI-101183;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000305|PubMed:31263175}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:O43402}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:O43402}.
CC -!- SIMILARITY: Belongs to the EMC8/EMC9 family. {ECO:0000305}.
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DR EMBL; AE013599; AAF46921.1; -; Genomic_DNA.
DR RefSeq; NP_611731.1; NM_137887.4.
DR AlphaFoldDB; Q9W1Y1; -.
DR SMR; Q9W1Y1; -.
DR BioGRID; 63246; 8.
DR DIP; DIP-22682N; -.
DR IntAct; Q9W1Y1; 5.
DR STRING; 7227.FBpp0071818; -.
DR PaxDb; Q9W1Y1; -.
DR PRIDE; Q9W1Y1; -.
DR EnsemblMetazoa; FBtr0071907; FBpp0071818; FBgn0034791.
DR GeneID; 37635; -.
DR KEGG; dme:Dmel_CG3501; -.
DR UCSC; CG3501-RA; d. melanogaster.
DR CTD; 37635; -.
DR FlyBase; FBgn0034791; EMC8-9.
DR VEuPathDB; VectorBase:FBgn0034791; -.
DR eggNOG; KOG3289; Eukaryota.
DR GeneTree; ENSGT00390000006738; -.
DR HOGENOM; CLU_087337_0_1_1; -.
DR InParanoid; Q9W1Y1; -.
DR OMA; HVTPMAE; -.
DR OrthoDB; 1284861at2759; -.
DR PhylomeDB; Q9W1Y1; -.
DR BioGRID-ORCS; 37635; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 37635; -.
DR PRO; PR:Q9W1Y1; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034791; Expressed in egg chamber and 25 other tissues.
DR ExpressionAtlas; Q9W1Y1; baseline and differential.
DR Genevisible; Q9W1Y1; DM.
DR GO; GO:0072546; C:EMC complex; IPI:FlyBase.
DR GO; GO:0012505; C:endomembrane system; HDA:FlyBase.
DR GO; GO:0090150; P:establishment of protein localization to membrane; IEA:GOC.
DR CDD; cd08060; MPN_UPF0172; 1.
DR InterPro; IPR005366; EMC8/9.
DR InterPro; IPR037518; MPN.
DR PANTHER; PTHR12941; PTHR12941; 1.
DR Pfam; PF03665; UPF0172; 1.
DR PROSITE; PS50249; MPN; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Membrane; Reference proteome.
FT CHAIN 1..203
FT /note="ER membrane protein complex subunit 8/9 homolog"
FT /id="PRO_0000221191"
FT DOMAIN 4..140
FT /note="MPN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
SQ SEQUENCE 203 AA; 22781 MW; 721C526F66D4B25E CRC64;
MCDYKVSERA YAKLIFHAAK YPHQAVNGLL LAEKTSKGSQ VEIVDAIPLF HQCLYVTPMA
EVALMLIDAH AEREGLVIAG YYAAPENFYD NQVDKTPAAK IADKIQENFK NACFVVVDNK
LMTLQHDRAA IQVFNCPGDS GARWSKAKFT LSQASDTLEG VSLLLKRGAM RDLVDFDNHL
DNPDKNWTND FLNQPLNDLQ KLY
