ID   EMC8_RAT                Reviewed;         207 AA.
AC   Q5FVL2;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=ER membrane protein complex subunit 8;
DE   AltName: Full=Neighbor of COX4;
GN   Name=Emc8; Synonyms=Cox4nb;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Ovary;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Part of the endoplasmic reticulum membrane protein complex
CC       (EMC) that enables the energy-independent insertion into endoplasmic
CC       reticulum membranes of newly synthesized membrane proteins.
CC       Preferentially accommodates proteins with transmembrane domains that
CC       are weakly hydrophobic or contain destabilizing features such as
CC       charged and aromatic residues. Involved in the cotranslational
CC       insertion of multi-pass membrane proteins in which stop-transfer
CC       membrane-anchor sequences become ER membrane spanning helices. It is
CC       also required for the post-translational insertion of tail-anchored/TA
CC       proteins in endoplasmic reticulum membranes. By mediating the proper
CC       cotranslational insertion of N-terminal transmembrane domains in an N-
CC       exo topology, with translocated N-terminus in the lumen of the ER,
CC       controls the topology of multi-pass membrane proteins like the G
CC       protein-coupled receptors. By regulating the insertion of various
CC       proteins in membranes, it is indirectly involved in many cellular
CC       processes. {ECO:0000250|UniProtKB:O43402}.
CC   -!- SUBUNIT: Component of the ER membrane protein complex (EMC). EMC8 and
CC       EMC9 are mutually exclusive subunits of the EMC complex.
CC       {ECO:0000250|UniProtKB:O43402}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:O43402}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:O43402}; Cytoplasmic side
CC       {ECO:0000250|UniProtKB:O43402}.
CC   -!- SIMILARITY: Belongs to the EMC8/EMC9 family. {ECO:0000305}.
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DR   EMBL; BC089914; AAH89914.1; -; mRNA.
DR   RefSeq; NP_001012165.1; NM_001012165.1.
DR   AlphaFoldDB; Q5FVL2; -.
DR   SMR; Q5FVL2; -.
DR   STRING; 10116.ENSRNOP00000023855; -.
DR   jPOST; Q5FVL2; -.
DR   PaxDb; Q5FVL2; -.
DR   PRIDE; Q5FVL2; -.
DR   Ensembl; ENSRNOT00000023855; ENSRNOP00000023855; ENSRNOG00000017654.
DR   Ensembl; ENSRNOT00000102818; ENSRNOP00000086027; ENSRNOG00000017654.
DR   GeneID; 361425; -.
DR   KEGG; rno:361425; -.
DR   UCSC; RGD:1311219; rat.
DR   CTD; 10328; -.
DR   RGD; 1311219; Emc8.
DR   eggNOG; KOG3289; Eukaryota.
DR   GeneTree; ENSGT00390000006738; -.
DR   HOGENOM; CLU_087337_0_1_1; -.
DR   InParanoid; Q5FVL2; -.
DR   OMA; HVTPMAE; -.
DR   OrthoDB; 1284861at2759; -.
DR   PhylomeDB; Q5FVL2; -.
DR   TreeFam; TF313860; -.
DR   PRO; PR:Q5FVL2; -.
DR   Proteomes; UP000002494; Chromosome 19.
DR   Bgee; ENSRNOG00000017654; Expressed in pancreas and 20 other tissues.
DR   Genevisible; Q5FVL2; RN.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0072546; C:EMC complex; ISS:UniProtKB.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:Ensembl.
DR   GO; GO:0032977; F:membrane insertase activity; IEA:Ensembl.
DR   GO; GO:0045050; P:protein insertion into ER membrane by stop-transfer membrane-anchor sequence; ISS:UniProtKB.
DR   GO; GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; ISS:UniProtKB.
DR   CDD; cd08060; MPN_UPF0172; 1.
DR   InterPro; IPR005366; EMC8/9.
DR   InterPro; IPR037518; MPN.
DR   PANTHER; PTHR12941; PTHR12941; 1.
DR   Pfam; PF03665; UPF0172; 1.
DR   PROSITE; PS50249; MPN; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Membrane; Reference proteome.
FT   CHAIN           1..207
FT                   /note="ER membrane protein complex subunit 8"
FT                   /id="PRO_0000328440"
FT   DOMAIN          4..147
FT                   /note="MPN"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
SQ   SEQUENCE   207 AA;  23406 MW;  FD21CCE8E9F8CDA3 CRC64;
     MPGVKLTTQA YCKMVLHGAK YPHCAVNGLL VAERQRPRKE HPPGAGNHTL FVDCIPLFHG
     TLALTPMLEV ALTLIDSWCK DNSYVIAGYY QANERVKDAS PNQVAEKVAS RIAEGFSDAA
     LIMVDNAKFT MDCAAPTIHV YEQHENRWRC RDPHHDYCED WPEAQRISAS LLDSRSYETL
     VDFDNHLDDI RSDWTNPEIN KAVLHLC