EMC_DROME
ID EMC_DROME Reviewed; 199 AA.
AC P18491; Q9W0N0;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 30-APR-2003, sequence version 2.
DT 03-AUG-2022, entry version 194.
DE RecName: Full=Protein extra-macrochaetae;
GN Name=emc; ORFNames=CG1007;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX PubMed=1690605; DOI=10.1016/0092-8674(90)90213-x;
RA Garrell J., Modolell J.;
RT "The Drosophila extramacrochaetae locus, an antagonist of proneural genes
RT that, like these genes, encodes a helix-loop-helix protein.";
RL Cell 61:39-48(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1690604; DOI=10.1016/0092-8674(90)90212-w;
RA Ellis H.M., Spann D.R., Posakony J.W.;
RT "Extramacrochaetae, a negative regulator of sensory organ development in
RT Drosophila, defines a new class of helix-loop-helix proteins.";
RL Cell 61:27-38(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Participates in sensory organ patterning by antagonizing the
CC neurogenic activity of the Achaete-scute complex (AS-C). It lacks a
CC basic DNA-binding domain but is able to form heterodimers with other
CC HLH proteins, thereby inhibiting DNA binding. May sequester proneural
CC proteins in complexes inefficient for DNA interaction. EMC also affects
CC vein differentiation. Inhibits the activity of AS-C proteins by forming
CC an non-DNA binding heterodimer.
CC -!- SUBUNIT: Heterodimer with other HLH proteins.
CC -!- INTERACTION:
CC P18491; P10084: sc; NbExp=4; IntAct=EBI-105737, EBI-174136;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M31900; AAA28510.1; -; Genomic_DNA.
DR EMBL; M31901; AAA28510.1; JOINED; Genomic_DNA.
DR EMBL; M31902; AAA28509.1; -; mRNA.
DR EMBL; M32637; AAA28511.1; -; Genomic_DNA.
DR EMBL; M32636; AAA28511.1; JOINED; Genomic_DNA.
DR EMBL; AE014296; AAF47413.2; -; Genomic_DNA.
DR EMBL; AY069405; AAL39550.1; -; mRNA.
DR PIR; A34688; A34688.
DR PIR; A34689; A34689.
DR RefSeq; NP_523876.2; NM_079152.4.
DR AlphaFoldDB; P18491; -.
DR SMR; P18491; -.
DR BioGRID; 63646; 30.
DR DIP; DIP-73N; -.
DR IntAct; P18491; 17.
DR STRING; 7227.FBpp0072477; -.
DR PaxDb; P18491; -.
DR EnsemblMetazoa; FBtr0072578; FBpp0072477; FBgn0000575.
DR GeneID; 38091; -.
DR KEGG; dme:Dmel_CG1007; -.
DR CTD; 38091; -.
DR FlyBase; FBgn0000575; emc.
DR VEuPathDB; VectorBase:FBgn0000575; -.
DR eggNOG; ENOG502RZP5; Eukaryota.
DR GeneTree; ENSGT00940000169907; -.
DR HOGENOM; CLU_115564_0_0_1; -.
DR InParanoid; P18491; -.
DR OMA; GRIQRHP; -.
DR OrthoDB; 1624054at2759; -.
DR PhylomeDB; P18491; -.
DR Reactome; R-DME-2559585; Oncogene Induced Senescence.
DR SignaLink; P18491; -.
DR BioGRID-ORCS; 38091; 1 hit in 3 CRISPR screens.
DR GenomeRNAi; 38091; -.
DR PRO; PR:P18491; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0000575; Expressed in wing disc and 82 other tissues.
DR Genevisible; P18491; DM.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005654; C:nucleoplasm; HDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0046982; F:protein heterodimerization activity; IPI:FlyBase.
DR GO; GO:0003714; F:transcription corepressor activity; IDA:FlyBase.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0008407; P:chaeta morphogenesis; TAS:FlyBase.
DR GO; GO:0030381; P:chorion-containing eggshell pattern formation; IMP:FlyBase.
DR GO; GO:0032922; P:circadian regulation of gene expression; IBA:GO_Central.
DR GO; GO:0042675; P:compound eye cone cell differentiation; IMP:FlyBase.
DR GO; GO:0007368; P:determination of left/right symmetry; IMP:FlyBase.
DR GO; GO:0046843; P:dorsal appendage formation; IMP:FlyBase.
DR GO; GO:0007391; P:dorsal closure; IMP:FlyBase.
DR GO; GO:0008258; P:head involution; IMP:FlyBase.
DR GO; GO:0061525; P:hindgut development; IMP:FlyBase.
DR GO; GO:0007476; P:imaginal disc-derived wing morphogenesis; IMP:FlyBase.
DR GO; GO:0008586; P:imaginal disc-derived wing vein morphogenesis; IMP:FlyBase.
DR GO; GO:0007494; P:midgut development; IMP:FlyBase.
DR GO; GO:0043392; P:negative regulation of DNA binding; IDA:FlyBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:FlyBase.
DR GO; GO:0046552; P:photoreceptor cell fate commitment; IMP:FlyBase.
DR GO; GO:0007458; P:progression of morphogenetic furrow involved in compound eye morphogenesis; IMP:FlyBase.
DR GO; GO:0048056; P:R3/R4 cell differentiation; IMP:FlyBase.
DR GO; GO:0045466; P:R7 cell differentiation; IMP:FlyBase.
DR GO; GO:0007530; P:sex determination; IGI:FlyBase.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR026052; DNA-bd_prot-inh.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR PANTHER; PTHR11723; PTHR11723; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..199
FT /note="Protein extra-macrochaetae"
FT /id="PRO_0000127169"
FT DOMAIN 23..75
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 127..199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 143..199
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 106
FT /note="Phosphoserine"
FT /evidence="ECO:0000255"
FT CONFLICT 123
FT /note="I -> V (in Ref. 1; AAA28510/AAA28509)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 199 AA; 21978 MW; 08C1683352B26F0E CRC64;
MKSLTAVCQT GASGMPALNA SGRIQRHPTH RGDGENAEMK MYLSKLKDLV PFMPKNRKLT
KLEIIQHVID YICDLQTELE THPEMGNFDA AAALTAVNGL HEDEDSDMED ADAEAEAEVD
PDILAQRLNA EQPAKVSSPA ARLPLTDRQT PNTLVAPAHP QQHQQQQQLQ LQQQQLQSQQ
QLSNSLATPQ NAEKDSRQS
