ID   3L2H_PSEAU              Reviewed;          89 AA.
AC   P14612; A8HDK5;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Long neurotoxin homolog Pa ID;
DE            Short=LNTX-ID;
DE   Flags: Precursor;
OS   Pseudechis australis (Mulga snake) (King brown snake).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Acanthophiinae; Pseudechis.
OX   NCBI_TaxID=8670;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=17906946; DOI=10.1007/s00018-007-7352-z;
RA   St Pierre L., Fischer H., Adams D.J., Schenning M., Lavidis N.,
RA   de Jersey J., Masci P.P., Lavin M.F.;
RT   "Distinct activities of novel neurotoxins from Australian venomous snakes
RT   for nicotinic acetylcholine receptors.";
RL   Cell. Mol. Life Sci. 64:2829-2840(2007).
RN   [2]
RP   PROTEIN SEQUENCE OF 22-89, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=2777741; DOI=10.1093/oxfordjournals.jbchem.a122797;
RA   Takasaki C.;
RT   "Amino acid sequence of a long-chain neurotoxin homologue, Pa ID, from the
RT   venom of an Australian elapid snake, Pseudechis australis.";
RL   J. Biochem. 106:11-16(1989).
CC   -!- FUNCTION: Binds with high affinity to muscular (alpha-1/CHRNA1) and
CC       neuronal (alpha-7/CHRNA7) nicotinic acetylcholine receptor (nAChR) and
CC       inhibits acetylcholine from binding to the receptor, thereby impairing
CC       neuromuscular and neuronal transmission.
CC       {ECO:0000250|UniProtKB:P60615}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:2777741}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MISCELLANEOUS: Does not show binding activity to the acetylcholine
CC       receptor of an electric ray eel nor lethal effect on mice.
CC       {ECO:0000269|PubMed:2777741}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Long-chain
CC       subfamily. Type II alpha-neurotoxin sub-subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; DQ917510; ABK63539.1; -; mRNA.
DR   PIR; JU0034; JU0034.
DR   AlphaFoldDB; P14612; -.
DR   SMR; P14612; -.
DR   PRIDE; P14612; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00206; snake_toxin; 1.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR003571; Snake_3FTx.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   SUPFAM; SSF57302; SSF57302; 1.
DR   PROSITE; PS00272; SNAKE_TOXIN; 1.
PE   1: Evidence at protein level;
KW   Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW   Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|PubMed:2777741"
FT   CHAIN           22..89
FT                   /note="Long neurotoxin homolog Pa ID"
FT                   /id="PRO_0000093562"
FT   DISULFID        24..42
FT                   /evidence="ECO:0000250"
FT   DISULFID        35..63
FT                   /evidence="ECO:0000250"
FT   DISULFID        48..52
FT                   /evidence="ECO:0000250"
FT   DISULFID        67..78
FT                   /evidence="ECO:0000250"
FT   DISULFID        79..84
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   89 AA;  10084 MW;  833F6834CFCB42C2 CRC64;
     MKTLLLTLVV VTIMCLDLGY TLTCYKGRDR SSETCRSEQE LCCTKTWCDQ WCQDRGPRLE
     MGCTATCPRR MPGLDFTCCT TDNCNPVPT