EME1A_ARATH
ID EME1A_ARATH Reviewed; 546 AA.
AC Q84M98; C5IHG3; Q9SJ19;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Crossover junction endonuclease EME1A;
DE EC=3.1.22.-;
DE AltName: Full=Essential meiotic endonuclease 1A;
DE Short=AtEME1A;
GN Name=EME1A; OrderedLocusNames=At2g21800; ORFNames=F7D8.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, AND INTERACTION WITH
RP MUS81.
RC STRAIN=cv. Columbia;
RX PubMed=19339504; DOI=10.1104/pp.109.136846;
RA Geuting V., Kobbe D., Hartung F., Duerr J., Focke M., Puchta H.;
RT "Two distinct MUS81-EME1 complexes from Arabidopsis process Holliday
RT junctions.";
RL Plant Physiol. 150:1062-1071(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Interacts with MUS81 to form a DNA structure-specific
CC endonuclease with substrate preference for branched DNA structures with
CC a 5'-end at the branch nick. Typical substrates include 3'-flap
CC structures, D-loops, replication forks, nicked Holliday junctions and
CC also intact Holliday junctions with a reduced efficiency. May be
CC required in mitosis for the processing of stalled or collapsed
CC replication fork intermediates. Plays a role in DNA repair and in
CC genotoxic stress-induced homologous recombination (HR) in somatic
CC cells. Mediates a subset of meiotic recombination events that are
CC insensitive to crossover interference. {ECO:0000269|PubMed:19339504}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305};
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000305};
CC -!- SUBUNIT: Forms a heterodimer with MUS81.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q84M98-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q84M98-2; Sequence=VSP_044051;
CC -!- SIMILARITY: Belongs to the EME1/MMS4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD20399.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; FJ936556; ACR43775.1; -; mRNA.
DR EMBL; AC007019; AAD20399.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC07223.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07224.1; -; Genomic_DNA.
DR EMBL; BT006456; AAP21264.1; -; mRNA.
DR EMBL; AK228007; BAE99972.1; -; mRNA.
DR PIR; C84605; C84605.
DR RefSeq; NP_001189572.1; NM_001202643.1. [Q84M98-2]
DR RefSeq; NP_179771.2; NM_127749.5. [Q84M98-1]
DR AlphaFoldDB; Q84M98; -.
DR SMR; Q84M98; -.
DR STRING; 3702.AT2G21800.2; -.
DR iPTMnet; Q84M98; -.
DR PaxDb; Q84M98; -.
DR PRIDE; Q84M98; -.
DR ProteomicsDB; 220496; -. [Q84M98-1]
DR EnsemblPlants; AT2G21800.1; AT2G21800.1; AT2G21800. [Q84M98-1]
DR EnsemblPlants; AT2G21800.2; AT2G21800.2; AT2G21800. [Q84M98-2]
DR GeneID; 816716; -.
DR Gramene; AT2G21800.1; AT2G21800.1; AT2G21800. [Q84M98-1]
DR Gramene; AT2G21800.2; AT2G21800.2; AT2G21800. [Q84M98-2]
DR KEGG; ath:AT2G21800; -.
DR Araport; AT2G21800; -.
DR TAIR; locus:2052484; AT2G21800.
DR eggNOG; ENOG502SDU4; Eukaryota.
DR InParanoid; Q84M98; -.
DR OrthoDB; 525637at2759; -.
DR PhylomeDB; Q84M98; -.
DR PRO; PR:Q84M98; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q84M98; baseline and differential.
DR Genevisible; Q84M98; AT.
DR GO; GO:0048476; C:Holliday junction resolvase complex; IEA:InterPro.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IDA:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IDA:TAIR.
DR GO; GO:0006281; P:DNA repair; IDA:TAIR.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR Gene3D; 1.10.150.670; -; 1.
DR InterPro; IPR042530; EME1/EME2_C.
DR InterPro; IPR006166; ERCC4_domain.
DR InterPro; IPR033310; Mms4/EME1/EME2.
DR PANTHER; PTHR21077; PTHR21077; 1.
DR Pfam; PF02732; ERCC4; 1.
DR SMART; SM00891; ERCC4; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Calcium; Cell cycle; Cell division; Coiled coil;
KW DNA damage; DNA recombination; DNA repair; Endonuclease; Hydrolase;
KW Magnesium; Meiosis; Metal-binding; Mitosis; Nuclease; Nucleus;
KW Reference proteome.
FT CHAIN 1..546
FT /note="Crossover junction endonuclease EME1A"
FT /id="PRO_0000418427"
FT DOMAIN 278..478
FT /note="ERCC4"
FT REGION 1..55
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 88..232
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 188..245
FT /evidence="ECO:0000255"
FT COMPBIAS 14..30
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 31..53
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..115
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 128..149
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 150..172
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 187..232
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 259
FT /note="G -> GVIT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19339504"
FT /id="VSP_044051"
SQ SEQUENCE 546 AA; 61239 MW; 4A586F27AE951311 CRC64;
MSDFILISDG EDEATPPPSK RARKNRTPTD LNLDTEPSLQ KQPPGSASTP FFLDETPLSD
DVTVLKSSFG SGTGASSGRE NNFFGKRVIS LESDSEDSPG PESSKKYEPV YTDSWKKPCR
LEFGSSDANS DDDPSWMRRA SFQSSLSKDA IEVDSDHEKE DTGVEKMGRK KQTITSKSTS
LSADSLPKKK MSKDEKTRAA EEKKLQKEQE KLQKAASKAE DAEHKKLERE KQKWAKEKDK
ALKCIVAWID NKVLEGSFGG LLISGLKEKC ITYHVTTNPI QRSIVWTMTL PEDIAQSLPL
GSKIPYVLLL YEAEDFCNLV AKKELLENVY RVRDEYPSYT MCYLTNKLLS YVNKKERVEY
KDPVNGCGWR KPPIDEAIAK LSTHYIGVHS RHCVDEAEVA DHVVRLTSSL AHCQVRKKLT
RLSVYADGTL MSKNAADKHL IRESIWLKVL VAIPKVQPRY AIAVSKKYPS LKSLLKVYMD
PNISVHEKEF LLKDLKVENL VGRDTSVGEA CSKRIYRVLM SLDGTIKTDD VENGAASFTL
PPSDLI
